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General Information
Symbol
Dmel\Sec5E13
Species
D. melanogaster
Name
FlyBase ID
FBal0146820
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C3458610T

Amino acid change:

Q361term | Sec5-PA

Reported amino acid change:

Q361term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q361term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adults in which eye tissue has been made homozygous for sec5E13 during development (using the EGUF method) show ablation of the eye.

sec5E13 mutant larvae display tracheal fusion defects in 20% of the examined tracheal dorsal branches.

Oocytes derived from sec5E13 germline clones show the normal large increase in nurse cell and oocyte size that occurs during oogenesis, but the final eggs are flaccid and often collapse after oviposition as a result of the lack of yolk granules that fill up the wild type egg.

The amount of yolk is reduced from approximately 35% of the cytoplasm in wild type eggs to approximately 4.1% of the cytoplasm in oocytes derived from sec5E13 germline clones.

Oocytes derived from sec5E13 germline clones have a reduced number of vesicles and tubules beneath the oocyte surface as well as an approximately 7-fold decrease in the number of clathrin-coated pits and vesicles compared to wild type.

Both the Golgi stack and the trans-Golgi network are approximately 2-fold larger in oocytes derived from sec5E13 germline clones compared to wild type.

Females carrying sec5E13 germ-line clones are fertile, but lay eggs in which the dorsal appendages are either too closely spaced or are fused. Some egg chambers in these animals lack membranes between some of the germ-cells, resulting in nurse cell nuclei falling into the oocyte and aggregation of ring canals. In some of these egg chambers the oocyte nucleus is mislocalized away from the dorsal membrane, although in all such cases the nucleus is localized in the anterior (as in wild-type).

The eye is completely ablated in mosaic animals in which the eye is homozygous for sec5E13. Females with homozygous germline clones do not produce eggs.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Sec5E13/RopG27 transheterozygous third instar larvae show decreased number of terminal dendritic branches in the class IV dendritic arborizing neurons compared to either Sec5E13/+ or RopG27/+ single heterozygotes.

The fraction of oocytes showing endocytic activity is reduced compared to wild type in EndoB54/Sec5E13 double heterozygotes.

The cuticle defects seen at 25[o]C in embryos derived from exo84onr/Df(3R)Espl3 females mated to exo84onr heterozygous males are enhanced by sec5E13 (both parents are heterozygous for the second mutation).

Expression of sec8Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4nos.PG in homozygous female sec5E13 germline clones results in egg chamber development arresting between stages 7 and 9, such that these females lay no eggs. The oocyte nucleus migrates appropriately to the anterior end of the cell in the double mutant germlines at stage 7, but the oocyte fails to enlarge, remaining comparable in size to the nurse cells. Some disruption of the nurse cell membranes is seen, with some cells fused and the ring canals clustered together.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (15)