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General Information
Symbol
Dmel\rols08232ex18
Species
D. melanogaster
Name
FlyBase ID
FBal0146840
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
P1729ex18
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{PZ}rols08232 has deleted approximately 2kB either side of the original insertion.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Significant myoblast fusion fails to occur in rols08232ex18/Df(3L)BK9 embryos - the average number of eve-positive nuclei within a DA1 muscle at stage 15 is 3.3 compared to 9.9 in wild type embryos. Most rols08232ex18/Df(3L)BK9 embryos fail to hatch and eventually die.

While a wild-type DA1 myotube has an average of 15 nuclei at stage 15 (n = 70), its equivalent in embryos homozygous for rols08232ex18 has an average of just 2 (n = 108).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of rols6.Scer\UAS, rolsΔ1-863.Scer\UAS.T:Ivir\HA1, rolsΔ1-1150.Scer\UAS.T:Ivir\HA1 or rolsΔ1401-1900.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4how-24B fails to rescue the myoblast fusion defect of rols08232ex18/Df(3L)BK9 embryos.

Expression of rols7.Scer\UAS, rolsScer\UAS.N.T:Ivir\HA1, rolsScer\UAS.C.T:Ivir\HA1, rolsΔ1-137.Scer\UAS.T:Ivir\HA1, rolsΔ1-264.Scer\UAS.T:Ivir\HA1, rolsΔ1-439.Scer\UAS.T:Ivir\HA1 or rolsS241A.S243A.Scer\UAS under the control of Scer\GAL4how-24B fully rescues the myoblast fusion defect of rols08232ex18/Df(3L)BK9 embryos.

Expression of rolsΔ1739-1900.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4how-24B partially rescues the myoblast fusion defect of rols08232ex18/Df(3L)BK9 embryos. However, a functional musculature is not produced, most of the embryos fail to hatch and eventually die.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
rols08232ex18
rols1729ex18
rolsP1729ex18
Name Synonyms
Secondary FlyBase IDs
    References (2)