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General Information
Symbol
Dmel\pygoS28
Species
D. melanogaster
Name
FlyBase ID
FBal0146871
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C31577413T

Amino acid change:

Q740term | pygo-PA

Reported amino acid change:

?740term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: ?740term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant embryos derived from homozygous female germline clones have a "lawn of denticles" cuticle phenotype.

pygoS28 mutant embryos that lack maternal and zygotic pygo function display a cuticle denticle lawn that is characteristic of a complete loss of wg signalling.

Somatic clones of pygoS28 homozygous cells in the wing pouch region of third instar wing discs are smaller on average than their wild-type twin spots (P<0.001). Mutant wing pouch clones are associated with increased apoptosis marker expression. When these clones fall in the notum region of this disc there is no difference between mutant clone and twin spot size.

Mutant embryos derived from homozygous female germline clones (lacking both maternal and zygotic pygo function) are short and lack the naked stretches that are normally interspersed between the ventral denticle belts ("lawn of denticles" phenotype). The middle gut constriction is missing. Homozygous clones result in loss of wing tissue, loss of the distal leg, dorsal duplication of the distal leg segments, nicking of the wing and wing margin defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The "lawn of denticles" cuticle phenotype seen in pygoS28 embryos is substantially rescued by expression of pan::pygopygoΔPHD-ΔNTCF.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.G32; a segmented pattern of denticle bands and naked cuticle is restored. The "lawn of denticles" cuticle phenotype seen in pygoS28 embryos is not rescued by expression of arm::panarmC.ΔNTCF.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.G32. The "lawn of denticles" cuticle phenotype seen in pygoS28 embryos is rescued by lgs::pygoΔPHD.HD2.αTub84B.

Overexpression of armScer\UAS.T:Hsap\MYC,T:SV40\nls2, under the regulation of Scer\GAL4da.G32 suppresses the mutant phenotype of pygoS28 embryos which lack both maternal and zygotic pygo function. The degree of suppression varies very little between individual embryos, and is essentially the same as that provided by zygotic pygo expressed from the paternal wild-type allele.

RpS3+; pygoS28 homozygous clones in a RpS32/+ background are 57% the size of RpS3+; pygo+ homozygous clones in a RpS32/+ background; i.e.- pygoS28 clones still have a growth disadvantage even when cell competition is suppressed.

Expression of armS10.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4arm.PS has no effect on the cuticle phenotype of pygoS28 embryos derived from pygoS28 germline clones.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (6)