Adulthood-generated pygoS123 homozygous somatic clones in mosaic midguts have a significant increase in cell number, as compared to adulthood-generated control somatic clones in wild-type midguts.
Generating pygoS123 homozygous somatic clones in mosaic midguts during adulthood leads to an increase in the numbers of neighboring control intestinal progenitor cells, heterozygous for pygoS123.
Haltere disc bearing clones mutant for pygoS123 show severe patterning defects due to clone overgrowth.
Embryos from mothers with pygoS123 mutant germline clones show a lawn of ventral denticles (a hallmark of wg signaling failure).
pygoS123 clones 42 hours after pupal formation have abnormal cone cell morphology compared to wild type but a normal cone cell number. Cell death levels (as visualised by Caspase-3 staining) are similar to controls.
Clones induced in the eye discs of pygoS123 third instar larvae display mild cone cell defects.
Homozygous embryos derived from homozygous female germ-line clones (lacking both maternal and zygotic pygo function) show a lawn of denticles phenotype). The regions of naked cuticle are largely restored in paternally rescued homozygous embryos (lacking only zygotic pygo function).
pygoS123 mutant embryos that lack maternal and zygotic pygo function display a cuticle denticle lawn that is characteristic of a complete loss of wg signalling.
Mutant embryos derived from homozygous female germline clones (lacking both maternal and zygotic pygo function) are short and lack the naked stretches that are normally interspersed between the ventral denticle belts ("lawn of denticles" phenotype). The middle gut constriction is missing.