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General Information
Symbol
Dmel\pygoS123
Species
D. melanogaster
Name
FlyBase ID
FBal0146872
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Small deletion close to the pygo translation initiation site, resulting in a truncation that retains only 51 amino acids.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adulthood-generated pygoS123 homozygous somatic clones in mosaic midguts have a significant increase in cell number, as compared to adulthood-generated control somatic clones in wild-type midguts.

Generating pygoS123 homozygous somatic clones in mosaic midguts during adulthood leads to an increase in the numbers of neighboring control intestinal progenitor cells, heterozygous for pygoS123.

Haltere disc bearing clones mutant for pygoS123 show severe patterning defects due to clone overgrowth.

Embryos from mothers with pygoS123 mutant germline clones show a lawn of ventral denticles (a hallmark of wg signaling failure).

pygoS123 clones 42 hours after pupal formation have abnormal cone cell morphology compared to wild type but a normal cone cell number. Cell death levels (as visualised by Caspase-3 staining) are similar to controls.

Clones induced in the eye discs of pygoS123 third instar larvae display mild cone cell defects.

Homozygous embryos derived from homozygous female germ-line clones (lacking both maternal and zygotic pygo function) show a lawn of denticles phenotype). The regions of naked cuticle are largely restored in paternally rescued homozygous embryos (lacking only zygotic pygo function).

pygoS123 mutant embryos that lack maternal and zygotic pygo function display a cuticle denticle lawn that is characteristic of a complete loss of wg signalling.

Mutant embryos derived from homozygous female germline clones (lacking both maternal and zygotic pygo function) are short and lack the naked stretches that are normally interspersed between the ventral denticle belts ("lawn of denticles" phenotype). The middle gut constriction is missing.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference

pygo[+]/pygoS123 is a suppressor of eye phenotype of armS56F.GMR

pygo[+]/pygoS123 is a suppressor | partially of wing phenotype of Chie5.5

pygo[+]/pygoS123 is a suppressor of phenotype of armS56F.GMR

Additional Comments
Genetic Interactions
Statement
Reference

The wing notch phenotype seen in Chie5.5/+ mutants is partially suppressed by the presence of pygoS123/+.

pygoS123/+ suppresses the rough eye phenotype seen in flies expressing armS56F.GMR.

pygoS123/+ suppresses the necrosis seen in adult eyes when NGD144 is driven by Scer\GAL4sev.PU.

pygoS123/+ suppresses the cone cell loss seen in the third instar eye discs when NGD144 is driven by Scer\GAL4sev.PU.

Overexpression of armScer\UAS.T:Hsap\MYC,T:SV40\nls2, under the regulation of Scer\GAL4da.G32 suppresses the mutant phenotype of pygoS123 embryos which lack both maternal and zygotic pygo function. The degree of suppression varies very little between individual embryos, and is essentially the same as that provided by zygotic pygo expressed from the paternal wild-type allele.

Expression of armS10.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4arm.PS has no effect on the cuticle phenotype of pygoS123 embryos derived from pygoS123 germline clones.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (15)