FlyBase Allele Report: Dmel\pav[UASp.mGFP6]

General Information

Symbol

Dmel\pav^UASp.mGFP6

Species

D. melanogaster

Name

FlyBase ID

FBal0146928

Feature type

allele

Associated gene

Dmel\pav

Associated Insertion(s)

Carried in Construct

P{UASp-GFP-pav}

Key Links

Transgenic product class

wild_type

(Minestrini et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Minestrini et al., 2002)

Mutagen

in vitro construct

(Minestrini et al., 2002)

Progenitor genotype

Carried in construct

P{UASp-GFP-pav}

Cytology

Description

UASp regulatory sequences drive expression of pav tagged at the N-terminal end with mGFP6.

(Minestrini et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

pav

Tagged with

mGFP6

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with Scer\GAL4^{VP16.mat.αTub67C}

(Minestrini et al., 2002)

Phenotype Manifest In

egg, with Scer\GAL4^{VP16.mat.αTub67C}

(Minestrini et al., 2002)

egg chamber, with Scer\GAL4^{VP16.mat.αTub67C}

(Minestrini et al., 2002)

nurse cell, with Scer\GAL4^{VP16.mat.αTub67C}

(Minestrini et al., 2002)

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

(Minestrini et al., 2002)

Detailed Description

Statement

Reference

Overexpression of pav^{Scer\UAS.P\T.T:Avic\GFP-m6} driven by either Scer\GAL4^e22c or Scer\GAL4^ap-md544 has little effect on viability or on epidermal patterning.

Overexpression of pav^{Scer\UAS.P\T.T:Avic\GFP-m6} driven by Scer\GAL4^ap-md544 does not alter wing size.

(Jones et al., 2010)

When tum^{Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EYFP} is driven by Scer\GAL4^OK107 gross mushroom body projection is unaffected.

(Goldstein et al., 2005)

The nurse cells are often not contained within the anterior half of the egg chamber in females expressing pav^{Scer\UAS.P\T.T:Avic\GFP-m6} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, and instead whole nurse cells or nurse cell nuclei protrude into the oocyte compartment. These mislocalised nurse cells do not regress as normally occurs when nurse cells dump their contents, resulting in the formation of eggs with several large nuclei. In up to 40% of egg chambers there is no fast cytoplasmic streaming from the nurse cells into the oocyte, the nurse cells fail to regress and the eggs never reach full size. In some egg chambers the oocyte sometimes fails to localise to the posterior pole.

(Minestrini et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^ap-md544, pav^UASp.mGFP6, tum^UAS.cSa has lethal | P-stage phenotype

(Jones et al., 2010)

Scer\GAL4^e22c, pav^UASp.mGFP6, tum^UAS.cSa has lethal | embryonic stage phenotype

(Jones et al., 2010)

Scer\GAL4^ey-OK107, pav^UASp.mGFP6, tum^{UAS.Tag:MYC,YFP} has abnormal neuroanatomy phenotype

(Goldstein et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^e22c, pav^UASp.mGFP6, tum^UAS.cSa has abdominal ventral denticle belt | embryonic stage phenotype, enhanceable by wg[+]/wg^l-17

(Jones et al., 2010)

Enhancer of

Statement

Reference

Scer\GAL4^ap-md544/pav^UASp.mGFP6 is an enhancer of wing phenotype of Scer\GAL4^ap-md544, tum^UAS.cSa

(Jones et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^arm.PS/pav^UASp.mGFP6 is a suppressor | partially of abdominal ventral denticle belt phenotype of Scer\GAL4^arm.PS, arm^{S10.UAS.Tag:MYC}

(Jones et al., 2010)

Other

Statement

Reference

Scer\GAL4^e22c, pav^UASp.mGFP6, tum^UAS.cSa has abdominal ventral denticle belt | embryonic stage phenotype

(Jones et al., 2010)

Scer\GAL4^ey-OK107, pav^UASp.mGFP6, tum^{UAS.Tag:MYC,YFP} has mushroom body & axon phenotype

(Goldstein et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of tum^Scer\UAS.cSa with pav^{Scer\UAS.P\T.T:Avic\GFP-m6} in the developing embryonic epidermis driven by Scer\GAL4^e22c results in embryonic lethality.

Co-expression of tum^Scer\UAS.cSa with pav^{Scer\UAS.P\T.T:Avic\GFP-m6} in the developing embryonic epidermis driven by Scer\GAL4^e22c causes reduction of the segmental naked cuticle expanse. The penetrance of this segmentation defect is increased when embryos are heterozygous for wg^l-17.

Co-expression of tum^Scer\UAS.cSa with pav^{Scer\UAS.P\T.T:Avic\GFP-m6} driven by Scer\GAL4^ap-md544 results in a profound decrease in eclosion rate. The few flies that eclose show wings that are reduced in size and blistered.

Co-expression of pav^{Scer\UAS.P\T.T:Avic\GFP-m6} driven by Scer\GAL4^arm.PS partially blocks the cell-fate transformation induced by arm^{S10.Scer\UAS.T:Hsap\MYC} expression and allows some denticle production.

(Jones et al., 2010)

When both tum^{Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EYFP} and pav^{Scer\UAS.P\T.T:Avic\GFP-m6} are driven in the mushroom body by Scer\GAL4^OK107, a high occurrence of axon misguidance is seen, often forming a cluster of axons in a ball like form. When both tum^{R417L.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EYFP} and pav^{Scer\UAS.P\T.T:Avic\GFP-m6} are driven in the mushroom body by Scer\GAL4^OK107, no axon guidance phenotype is seen.

(Goldstein et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^e22c/pav^UASp.mGFP6 rescues pav^B200

(Jones et al., 2010)

Comments

Expression of pav^{Scer\UAS.P\T.T:Avic\GFP-m6} driven by Scer\GAL4^e22c rescues the epidermal pattern defects of pav^B200 mutants.

(Jones et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pav^{Scer\UAS.P\T.T:Avic\GFP-m6}

pav^UASp.mGFP6

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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