FB2025_01 , released February 20, 2025
Allele: Dmel\pavNLS4-7*.UASp.mGFP6
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General Information
Symbol
Dmel\pavNLS4-7*.UASp.mGFP6
Species
D. melanogaster
Name
FlyBase ID
FBal0146931
Feature type
allele
Associated gene
Dmel\pav
Associated Insertion(s)
Carried in Construct
P{UASp-GFP-pav.NLS4-7*}
Key Links
Transgenic product class
missense_variant
(Minestrini et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Minestrini et al., 2002)
Mutagen
in vitro construct
(Minestrini et al., 2002)
Progenitor genotype
Carried in construct
P{UASp-GFP-pav.NLS4-7*}
Cytology
Description

UASp regulatory sequences drive expression of pav, with mutations in putative nuclear localization sequences 4-7 (amino acid replacements K770A, N771A, R772A, R843A, K844A and R845A), tagged at the N-terminal end with mGFP6.

(Minestrini et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
pav
Tagged with
mGFP6
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When pavNLS4-7*.Scer\UAS.P\T.T:Avic\GFP-m6 is driven by Scer\GAL4OK107 no gross morphological changes in the mushroom body axons is seen.

      (Goldstein et al., 2005)

      Egg chambers of females expressing pavNLS4-7*.Scer\UAS.P\T.T:Avic\GFP-m6 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 contain a dense filamentous network of microtubules in the cytoplasm and at the cortex of nurse cells as well as in ring canals. The egg chamber develops a single oocyte, but it becomes mispositioned. Egg chambers containing 32 cells are often seen in the ovaries of females expressing pavNLS4-7*.Scer\UAS.P\T.T:Avic\GFP-m6 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16. These 32-cell cysts each have two independent oocytes each with 4 ring canals, indicating that they are produced by the fusion of two egg chambers. In some cases all the nurse cells within the 32-cell cyst are similar in size, although there are some cases where 32-cell cysts contain fused egg chambers of different ages. The collapse of ring canals and breakdown of the actin skeleton between nurse cells is seen.

      (Minestrini et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Other
      Statement
      Reference

      Scer\GAL4ey-OK107, pavNLS4-7*.UASp.mGFP6, tumUAS.Tag:MYC,YFP has mushroom body & axon phenotype

      (Goldstein et al., 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of pavNLS4-7*.Scer\UAS.P\T.T:Avic\GFP-m6 driven by Scer\GAL4arm.PS is unable to block the cell-fate transformation induced by armS10.Scer\UAS.T:Hsap\MYC expression.

      (Jones et al., 2010)

      When both tumScer\UAS.T:Hsap\MYC,T:Avic\GFP-EYFP and pavNLS4-7*.Scer\UAS.P\T.T:Avic\GFP-m6 are driven in the mushroom body by Scer\GAL4OK107, a high occurrence of axon misguidance is seen.

      (Goldstein et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      pavNLS4-7*.UASp.mGFP6/Scer\GAL4e22c fails to rescue pavB200

      (Jones et al., 2010)
      Comments

      Expression of pavNLS4-7*.Scer\UAS.P\T.T:Avic\GFP-m6 driven by Scer\GAL4e22c fails to rescue the epidermal pattern defects of pavB200 mutants.

      (Jones et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      pavNLS4-7*.Scer\UAS.P\T.T:Avic\GFP-m6
      pavNLS4-7*.UASp.mGFP6
      Name Synonyms
      Secondary FlyBase IDs
        References (4)