Allele Dmel\p53^-ns.5'

General Information
Symbol	Dmel\p53^-ns.5'	Species	D. melanogaster
Name		FlyBase ID	FBal0146963
Feature type	allele	Associated gene	Dmel\p53
Also Known As	Dmp53^NS, dp53^ns, p53^-ns

Allele class
Mutagen	SCEI endonuclease, FLPase, ends-in gene targeting
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ends-in gene targeting (Sogame et al., 2003) FLPase (Sogame et al., 2003) SCEI endonuclease (Sogame et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	p53^{2.8.Scer\SceI.RS} (Sogame et al., 2003)
Nature of the lesion	Statement Reference Consists of sequences derived from both the endogenous p53 gene and the mutated p53^{2.8.Scer\SceI.RS} from P{p53.TV2}. Exons 1 to 6 appear wild-type, however following exon 6 are vector sequences followed by another exon 6 and the first part of exon 7. (Sogame et al., 2003)
Caused by aberration	Dp(3;3)p53^-ns (Tue et al., 2011, Sogame et al., 2003, Bauer et al., 2005, Rebollar et al., 2006, Trong-Tue et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	cell death defective (with p53^-ns.3') (Sogame et al., 2003) radiation sensitive (with p53^-ns.3') (Sogame et al., 2003, Rebollar et al., 2006) short lived (with p53^-ns.3') (Bauer et al., 2005)
Phenotype Manifest In

Detailed Description
	Statement Reference p53[-ns.5'] heterozygous mutants show apparently normal thorax structure. (Tue et al., 2011) p53^-ns.3',p53^-ns.5' mutants are viable, but sickly with a shortened life span. (Bauer et al., 2005) p53^-ns.3',p53^-ns.5' mutants do not exhibit the normal increase in apoptosis following irradiation. However cell cycle arrest occurs as normal. Low doses of ionising radiation cause an increase in genomic instability and an increase in lethality when compared to controls. (Sogame et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference p53^-ns.3'/p53^-ns.5' is a suppressor of increased cell death phenotype of Dref^Scer\UAS.cSa, Scer\GAL4^GMR.PS (Trong-Tue et al., 2010)
Other
Statement Reference BacA\p35^Scer\UAS.cHa, Scer\GAL4^hh-Gal4, W^Scer\UAS.cYa, p53^-ns.3', p53^-ns.5' has mitotic cell cycle defective phenotype (Wells et al., 2006)
Phenotype Manifest In
Enhancer of
Statement Reference p53[+]/p53^-ns.5' is an enhancer of adult thorax phenotype of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ}, Scer\GAL4^pnr-MD237 (Tue et al., 2011)
Suppressor of
Statement Reference p53^-ns.3'/p53^-ns.5' is a suppressor of eye phenotype of Dref^Scer\UAS.cSa, Scer\GAL4^GMR.PS (Trong-Tue et al., 2010) p53^-ns.3'/p53^-ns.5' is a suppressor of photoreceptor cell phenotype of Dref^Scer\UAS.cSa, Scer\GAL4^GMR.PS (Trong-Tue et al., 2010)
Other
Statement Reference BacA\p35^Scer\UAS.cHa, Scer\GAL4^hh-Gal4, W^Scer\UAS.cYa, p53^-ns.3', p53^-ns.5' has wing disc phenotype (Wells et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference A half reduction in the levels of p53 through crossing Scer\GAL4[GMR.PS]/+; Dref[Scer\UAS.cSa]/+ flies with p53[-ns.3'] p53[-ns.5'] flies suppresses the Dref[Scer\UAS.cSa]-induced rough eye phenotype. This suppression is more evident in a p53[-ns.3'] p53[-ns.5'] homozygous background. (Trong-Tue et al., 2010) The mutant eye phenotype caused by expression of AIF[ΔN.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[ey.PH] is not ameliorated if they are also homozygous for Dp(3;3)p53[-ns] (which carries p53[-ns.5'] and p53[-ns.3']). (Joza et al., 2008)
Xenogenetic Interactions
Statement Reference The increase in the number of TUNEL-positive cells and the non-autonomous increase in BrdU incorporation seen in the wing discs in larvae expressing Rcom\RA[cs2.Scer\UAS] under the control of Scer\GAL4[en-e16E] at 30[o]C is largely suppressed by Dp(3;3)p53[-ns]. (Mesquita et al., 2010) The cell cycle arrest and undergrowth phenotype of 96 hour wing discs with posterior 'undead' Scer\GAL4^hh-Gal4>W^Scer\UAS.cYa, BacA\p35^Scer\UAS.cHa cells is completely alleviated in both p53^5A-1-4 and p53^-ns.3', p53^-ns.5' backgrounds. (Wells et al., 2006)
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	23283 w¹¹¹⁸; Dp(3;3)p53^-ns, p53^-ns.5' w^+mW.hs p53^-ns.3'
Notes on Origin
Discoverer
	Induced with: p53^-ns.3'. Created by "ends-in" homologous recombination of the P{p53.TV2} transgene construct induced by Scer\FLP1^hs.PP and Scer\SCEI^hs.PR producing two alleles p53^-ns.5' and p53^-ns.3' in the tandem duplication Dp(3;3)p53^-ns. (Sogame et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	Dmp53^-ns (Sogame et al., 2003) Dmp53^NS (Bauer et al., 2005, Akdemir et al., 2007) dp53^ns (Wells et al., 2006, Mesquita et al., 2010) p53^-ns.5' p53^-ns (Trong-Tue et al., 2010, Yamada et al., 2008) p53^ns (Joza et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Tue et al., 2011, Gene 473(1): 1--7 NF-Y transcriptionally regulates the Drosophila p53 gene. [FBrf0212943] Mesquita et al., 2010, PLoS Biol. 8(12): e1000566 A dp53-Dependent Mechanism Involved in Coordinating Tissue Growth in Drosophila. [FBrf0212630] Trong-Tue et al., 2010, Oncogene 29(14): 2060--2069 Role of DREF in transcriptional regulation of the Drosophila p53 gene. [FBrf0210535] Joza et al., 2008, Cell Death Differ. 15(6): 1009--1018 The molecular archaeology of a mitochondrial death effector: AIF in Drosophila. [FBrf0205458] Yamada et al., 2008, Development 135(2): 207--216 Programmed cell death of primordial germ cells in Drosophila is regulated by p53 and the Outsiders monocarboxylate transporter. [FBrf0201242] Akdemir et al., 2007, Oncogene 26(36): 5184--5193 p53 directs focused genomic responses in Drosophila. [FBrf0202061] Rebollar et al., 2006, FEBS Lett. 580(2): 642--648 Role of the p53 homologue from Drosophila melanogaster in the maintenance of histone H3 acetylation and response to UV-light irradiation. [FBrf0190489] Wells et al., 2006, Curr. Biol. 16(16): 1606--1615 Compensatory proliferation in Drosophila imaginal discs requires Dronc-dependent p53 activity. [FBrf0192041] Sogame et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100(8): 4696--4701 Drosophila p53 preserves genomic stability by regulating cell death. [FBrf0159299]
Supplementary material	Bauer et al., 2005, Curr. Biol. 15(22): [FBrf0198673]

Allele Dmel\p53-ns.5'

Allele Dmel\p53^-ns.5'