FB2025_01 , released February 20, 2025
Allele: Dmel\numb15
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General Information
Symbol
Dmel\numb15
Species
D. melanogaster
Name
FlyBase ID
FBal0146969
Feature type
allele
Associated gene
Dmel\numb
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Le Borgne and Schweisguth, 2003)

loss of function allele

(Bhalerao et al., 2005)
Mutagen
ethyl methanesulfonate
(Berdnik et al., 2002)
Progenitor genotype
Cytology
Description
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      bract | somatic clone

      (Peng et al., 2012)

      centrosome | somatic clone

      (Jauffred et al., 2013)

      dMP2 neuron

      (Bhalerao et al., 2005)

      embryonic/larval optic lobe | larval stage (with numbNP2301)

      (Komori et al., 2018)

      external sensory organ precursor cell I | somatic clone

      (Jauffred et al., 2013)

      ganglion mother cell GMC4-2a

      (Bhalerao et al., 2005)

      hypodermal muscle cell of larval abdominal 9

      (Bhalerao et al., 2005)

      larval brain | somatic clone

      (Wirtz-Peitz et al., 2008)

      larval neuroblast | somatic clone

      (Wirtz-Peitz et al., 2008)

      larval neuroblast | somatic clone | third instar larval stage

      (Toriya et al., 2006)

      larval outer optic anlage | somatic clone | third instar larval stage

      (Toriya et al., 2006)

      larval RP2 motor neuron | increased number

      (Bhalerao et al., 2005)

      larval ventral nerve cord | somatic clone

      (Wirtz-Peitz et al., 2008)

      macrochaeta | somatic clone

      (Berdnik et al., 2002)

      mechanosensory sensory organ | somatic clone

      (Peng et al., 2012)

      muscle cell of A1-7 dorsal acute muscle 1

      (Bhalerao et al., 2005)

      muscle cell of A1-7 dorsal oblique muscle 1

      (Bhalerao et al., 2005)

      muscle cell of A1-7 dorsal oblique muscle 2

      (Bhalerao et al., 2005)

      muscle cell of abdominal 1 dorsal oblique muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 2 dorsal acute muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 2 dorsal oblique muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 2 dorsal oblique muscle 2

      (Bhalerao et al., 2005)

      muscle cell of abdominal 3 dorsal acute muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 3 dorsal oblique muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 3 dorsal oblique muscle 2

      (Bhalerao et al., 2005)

      muscle cell of abdominal 4 dorsal acute muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 4 dorsal oblique muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 4 dorsal oblique muscle 2

      (Bhalerao et al., 2005)

      muscle cell of abdominal 5 dorsal acute muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 5 dorsal oblique muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 5 dorsal oblique muscle 2

      (Bhalerao et al., 2005)

      muscle cell of abdominal 6 dorsal acute muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 6 dorsal oblique muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 6 dorsal oblique muscle 2

      (Bhalerao et al., 2005)

      muscle cell of abdominal 7 dorsal acute muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 7 dorsal oblique muscle 1

      (Bhalerao et al., 2005)

      muscle cell of abdominal 7 dorsal oblique muscle 2

      (Bhalerao et al., 2005)

      muscle cell of abdominal 8 dorsal oblique muscle 1

      (Bhalerao et al., 2005)

      muscle cell of mesothoracic dorsal oblique muscle 2

      (Bhalerao et al., 2005)

      muscle cell of prothoracic dorsal oblique muscle 2

      (Bhalerao et al., 2005)

      neuroblast | somatic clone | increased number

      (Ouyang et al., 2011, Ouyang et al., 2011)

      neuroblast | supernumerary & larval brain | somatic clone

      (Wang et al., 2006)

      neuroblast MP2

      (Bhalerao et al., 2005)

      neuron | somatic clone | third instar larval stage

      (Toriya et al., 2006)

      pericardial cell | increased number

      (Bhalerao et al., 2005)

      RP2sib neuron

      (Bhalerao et al., 2005)

      sensory neuron | embryonic stage

      (Bhalerao et al., 2005)

      tormogen cell | ectopic | somatic clone

      (Berdnik et al., 2002)

      tormogen cell | somatic clone

      (Peng et al., 2012)

      type II neuroblast | increased number (with numbEx112)

      (Rajan et al., 2023)

      type II neuroblast | increased number | larval stage (with numbNP2301)

      (Komori et al., 2018)

      vMP2 neuron | increased number

      (Bhalerao et al., 2005)
      Detailed Description
      Statement
      Reference

      numb15 clones generated in the larval eye disc do not result in ommatidial rotation phenotypes (planar cell polarity defects).

      (Domingos et al., 2019)

      numb15/numbNP2301 transheterozygosity leads to supernumerary type II neuroblasts in the larval optic lobe.

      (Komori et al., 2018)

      Centrosome asymmetry during cytokinesis in mutant pI (sensory organ precursor) cells in the notum is strongly reduced compared to that seen in wild-type pI cells, as the posterior centrosome movement towards the cell apex is delayed in the mutant cells.

      (Jauffred et al., 2013)

      Homozygous clones in the sensory organs of the leg produce four socket cells. These sensory organs fail to induce bracts. The mutant sensory organ socket cells do not show the protrusions normally seen projecting from the basolateral surface of the proximal side of wild-type socket cells.

      (Peng et al., 2012)

      Ectopic neuroblast formation is seen in numb15 mutant clones.

      (Ouyang et al., 2011)

      Homozygous clones in the optic lobe, induced during the first or early-second instar maintain neuroepithelial cell identity until the late-third instar and the differentiation of neuroepithelial cells into medulla neuroblasts is inhibited.

      (Wang et al., 2011)

      Homozygous intestinal stem cell clones form large clones over time, as do wild-type controls.

      (Bardin et al., 2010)

      Homozygous clones in the larval brain show strong overproliferation in the PAN neuroblast lineage and weaker overproliferation in the ase[+] neuroblast lineage. Homozygous clones in the ventral nerve cord show neuroblast overproliferation.

      (Wirtz-Peitz et al., 2008)

      numb15/numb15 clones have neuronal differentiation defects in the outer optic anlage of third instar larvae.

      (Toriya et al., 2006)

      Homozygous neuroblast clones in the larval brain contain a greater number of cells compared to wild-type clones.

      (Wang et al., 2006)

      In numb15 embryos, 54% of all hemisegments show a complete loss of sensory neurons. In numb15 embryos, the midline neuroblast MP2 divides to give two vMP2 cells, instead of a vMP2 and a dMP2 neuron. In numb15 embryos, the GMC4-2a divides to give two RP2sib cells, instead of one RP2 neuron and one RP2sib cell. In numb15 stage 14-15 embryos, the DA1 precursors and DO1 precursors are absent, as FDA1 and FDO1 are transformed into their sibling cells. In numb15 embryos, the P2 precursor divides symmetrically and generates four pericardial cells, instead of dividing asymmetrically and giving rise to a DO2 muscle founder and a sibling that divides into two pericardial cells.

      (Bhalerao et al., 2005)

      In somatic clones of numb15 in the head, almost all bristles are transformed into four sockets.

      (Berdnik et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Statement
      Reference

      numb[+]/numb15 is a suppressor of abnormal planar polarity | male | adult stage phenotype of dsh1

      (Domingos et al., 2019)
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Statement
      Reference

      numb[+]/numb15 is a suppressor of trichome of the posterior wing margin phenotype of Scer\GAL4en.PU, bazJF01078

      (Wu et al., 2024)

      numb[+]/numb15 is a suppressor of trichome of the posterior wing margin phenotype of Scer\GAL4en.PU, bazJF01079

      (Wu et al., 2024)

      numb[+]/numb15 is a suppressor of ommatidium | male phenotype of dsh1

      (Domingos et al., 2019)
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      numb15, ftQ805X double mutant clones generated in the larval eye disc lead to frequent ommatidial rotation phenotypes - planar cell polarity defects - both in the larva and in adults.

      The few ftQ805X/ft8, numb15/+ adult escapers show medial cross-vein defect (Fig. 6B) and PCP defects in the eye.

      (Domingos et al., 2019)

      Co-expression of bansponge.Scer\UAS.T:Disc\RFP in combination with the presence of numb15/+ fails to suppress the ectopic neuroblast formation and increased neuroblast numbers seen in larval brains expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

      The presence of numb15/+ fails to suppress the increased neuroblast number seen in larval brains expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

      (Wu et al., 2017)

      Klp98AΔ47/+;numb2/numbSW or Klp98AΔ47/+;numbSW/numb15 mutants have a multiple socket phenotype on the notum; this phenotype is suppressed in Klp98AΔ47/Klp98AΔ6;numb2/numbSW, Klp98AΔ47/Klp98AΔ8;numb2/numbSW or Klp98AΔ47/Klp98AΔ6;numb15/numbSW double mutants.

      (Derivery et al., 2015)

      Expression of p53Scer\UAS.Ex in numb15 mutant clones under the control of Scer\GAL4insc-Mz1407 effectively inhibits ectopic neuroblast formation.

      Co-expression of CycEScer\UAS.cUa and p53Scer\UAS.Ex generates a significant increase in ectopic neuroblasts in a numb15 mutant background, compared to overexpression of p53Scer\UAS.Ex alone in a numb15 mutant background.

      Expression of agoΔF.Scer\UAS under the control of Scer\GAL4insc-Mz1407 reduces the number of ectopic neuroblasts by approximately 36% in a numb15 mutant background.

      (Ouyang et al., 2011)

      The modest overproliferation of neuroblasts seen in the larval brain in animals expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 18[o]C is enhanced by numb15/+.

      The overproliferation of larval brain neuroblasts seen in l(2)gl1/l(2)gl4 animals is enhanced by numb15/+.

      (Wirtz-Peitz et al., 2008)

      In numb15; CycAC8LR1 double mutants, GMC4-2a differentiates into a RP2sib in 85% of cases.

      (Bhalerao et al., 2005)

      When numb15 somatic mutant clones are made in the thorax of animals expressing ApplScer\UAS.cTa (driven by Scer\GAL4sca-537.4), mechanosensory organs are seen that are made up of four socket cells.

      (Merdes et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\NUMBScer\UAS.PTBS-PRRS driven by Scer\GAL4tub.PU suppresses (whereas expression of Hsap\NUMBScer\UAS.PTBS-PRRL only partially suppresses) neuronal differentiation defects seen in the outer optic anlage of numb15/numb15 clones in third instar larvae.

      (Toriya et al., 2006)
      Complementation and Rescue Data
      Fails to complement

      numbS52F

      (Bhalerao et al., 2005)
      Rescued by

      numb2/numb15 is rescued by numb+tCH322-113H20

      (Couturier et al., 2013)

      numb2/numb15 is rescued by numbGFP

      (Couturier et al., 2013)

      numb15 is rescued by Scer\GAL4insc-Mz1407/numbUAS.cOa

      (Ouyang et al., 2011)

      numb15 is rescued by Scer\GAL4insc-Mz1407/numbTS2A.UAS

      (Ouyang et al., 2011)

      numb15 is rescued by Scer\GAL4Tub.PU/numbUAS.cWa

      (Toriya et al., 2006)
      Not rescued by

      numb15 is not rescued by Scer\GAL4insc-Mz1407/numbTS4D.UAS

      (Ouyang et al., 2011)
      Comments

      Expression of numbScer\UAS.cOa or numbTS4A.Scer\UAS suppresses the ectopic neuroblast formation seen in numb15 mutant clones.

      Expression of numbTS4D.Scer\UAS fails to suppress the ectopic neuroblast formation seen in numb15 mutant clones.

      (Ouyang et al., 2011)

      Expression of numbScer\UAS.cWa rescues neuronal differentiation defects seen in the outer optic anlage of numb15/numb15 third instar larvae.

      (Toriya et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      ftQ805X has been identified as a second-site mutation on the numb15 chromosome.

      (Domingos et al., 2019)

      Separable from: ftQ805X.

      (Domingos et al., 2019)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      References (29)