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General Information
Symbol
Dmel\numb15
Species
D. melanogaster
Name
FlyBase ID
FBal0146969
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

neuroblast | supernumerary & larval brain | somatic clone

Detailed Description
Statement
Reference

numb15 clones generated in the larval eye disc do not result in ommatidial rotation phenotypes (planar cell polarity defects).

Centrosome asymmetry during cytokinesis in mutant pI (sensory organ precursor) cells in the notum is strongly reduced compared to that seen in wild-type pI cells, as the posterior centrosome movement towards the cell apex is delayed in the mutant cells.

Homozygous clones in the sensory organs of the leg produce four socket cells. These sensory organs fail to induce bracts. The mutant sensory organ socket cells do not show the protrusions normally seen projecting from the basolateral surface of the proximal side of wild-type socket cells.

Ectopic neuroblast formation is seen in numb15 mutant clones.

Homozygous clones in the optic lobe, induced during the first or early-second instar maintain neuroepithelial cell identity until the late-third instar and the differentiation of neuroepithelial cells into medulla neuroblasts is inhibited.

Homozygous intestinal stem cell clones form large clones over time, as do wild-type controls.

Homozygous clones in the larval brain show strong overproliferation in the PAN neuroblast lineage and weaker overproliferation in the ase[+] neuroblast lineage. Homozygous clones in the ventral nerve cord show neuroblast overproliferation.

numb15/numb15 clones have neuronal differentiation defects in the outer optic anlage of third instar larvae.

Homozygous neuroblast clones in the larval brain contain a greater number of cells compared to wild-type clones.

In numb15 embryos, 54% of all hemisegments show a complete loss of sensory neurons. In numb15 embryos, the midline neuroblast MP2 divides to give two vMP2 cells, instead of a vMP2 and a dMP2 neuron. In numb15 embryos, the GMC4-2a divides to give two RP2sib cells, instead of one RP2 neuron and one RP2sib cell. In numb15 stage 14-15 embryos, the DA1 precursors and DO1 precursors are absent, as FDA1 and FDO1 are transformed into their sibling cells. In numb15 embryos, the P2 precursor divides symmetrically and generates four pericardial cells, instead of dividing asymmetrically and giving rise to a DO2 muscle founder and a sibling that divides into two pericardial cells.

In somatic clones of numb15 in the head, almost all bristles are transformed into four sockets.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

numb[+]/numb15 is a suppressor | partially of abnormal planar polarity | male | adult stage phenotype of dsh1

NOT Suppressor of
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference

numb[+]/numb15 is a suppressor | partially of ommatidium | male phenotype of dsh1

NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

numb15, ftQ805X double mutant clones generated in the larval eye disc lead to frequent ommatidial rotation phenotypes - planar cell polarity defects - both in the larva and in adults.

The few ftQ805X/ft8, numb15/+ adult escapers show medial cross-vein defect (Fig. 6B) and PCP defects in the eye.

Co-expression of bansponge.Scer\UAS.T:Disc\RFP in combination with the presence of numb15/+ fails to suppress the ectopic neuroblast formation and increased neuroblast numbers seen in larval brains expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

The presence of numb15/+ fails to suppress the increased neuroblast number seen in larval brains expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

Klp98AΔ47/+;numb2/numbSW or Klp98AΔ47/+;numbSW/numb15 mutants have a multiple socket phenotype on the notum; this phenotype is suppressed in Klp98AΔ47/Klp98AΔ6;numb2/numbSW, Klp98AΔ47/Klp98AΔ8;numb2/numbSW or Klp98AΔ47/Klp98AΔ6;numb15/numbSW double mutants.

Expression of p53Scer\UAS.Ex in numb15 mutant clones under the control of Scer\GAL4insc-Mz1407 effectively inhibits ectopic neuroblast formation.

Co-expression of CycEScer\UAS.cUa and p53Scer\UAS.Ex generates a significant increase in ectopic neuroblasts in a numb15 mutant background, compared to overexpression of p53Scer\UAS.Ex alone in a numb15 mutant background.

Expression of agoΔF.Scer\UAS under the control of Scer\GAL4insc-Mz1407 reduces the number of ectopic neuroblasts by approximately 36% in a numb15 mutant background.

The modest overproliferation of neuroblasts seen in the larval brain in animals expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 18[o]C is enhanced by numb15/+.

The overproliferation of larval brain neuroblasts seen in l(2)gl1/l(2)gl4 animals is enhanced by numb15/+.

In numb15; CycAC8LR1 double mutants, GMC4-2a differentiates into a RP2sib in 85% of cases.

When numb15 somatic mutant clones are made in the thorax of animals expressing ApplScer\UAS.cTa (driven by Scer\GAL4sca-537.4), mechanosensory organs are seen that are made up of four socket cells.

Xenogenetic Interactions
Statement
Reference

Expression of Hsap\NUMBScer\UAS.PTBS-PRRS driven by Scer\GAL4tub.PU suppresses (whereas expression of Hsap\NUMBScer\UAS.PTBS-PRRL only partially suppresses) neuronal differentiation defects seen in the outer optic anlage of numb15/numb15 clones in third instar larvae.

Complementation and Rescue Data
Fails to complement
Not rescued by
Comments

Expression of numbScer\UAS.cOa or numbTS4A.Scer\UAS suppresses the ectopic neuroblast formation seen in numb15 mutant clones.

Expression of numbTS4D.Scer\UAS fails to suppress the ectopic neuroblast formation seen in numb15 mutant clones.

Expression of numbScer\UAS.cWa rescues neuronal differentiation defects seen in the outer optic anlage of numb15/numb15 third instar larvae.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

ftQ805X has been identified as a second-site mutation on the numb15 chromosome.

Separable from: ftQ805X.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (25)