Allele Dmel\egr^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\egr^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0147162
Feature type	allele	Associated gene	Dmel\egr

Allele class
Mutagen	in vitro construct
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct (Igaki et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of a head to head inverted repeat of the egr coding sequence. (Igaki et al., 2002)
Carried in construct	P{UAS-eiger.IR} (Igaki et al., 2002, Polaski et al., 2006, Neisch et al., 2010, Keller et al., 2011, Wu et al., 2009, Ohsawa et al., 2011, Mabery and Schneider, 2010, Marchal et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	feeding behavior defective, with Scer\GAL4^Hml.PG (Mabery and Schneider, 2010) feeding behavior defective, with Scer\GAL4^Lsp2.PH (Mabery and Schneider, 2010) immune response defective, with Scer\GAL4^Lsp2.PH (Mabery and Schneider, 2010)
Phenotype Manifest In

Detailed Description
	Statement Reference Knockdown of egr in the fat body (through expression of egr[dsRNA.Scer\UAS] under the control of Scer\GAL4[Lsp2.PH]) significantly increases survival time during an Salmonella typhimurium infection. However, bacterial loads over this time remain unchanged, indicating that the extended survival is due to tolerance rather than resistance. Knockdown of egr in hemocytes (through expression of egr[dsRNA.Scer\UAS] under the control of Scer\GAL4[Hml.PG]) does not affect fly survival when infected by Salmonella typhimurium. Knockdown of egr in the fat body or hemocytes (through expression of egr[dsRNA.Scer\UAS] under the control of Scer\GAL4[Lsp2.PH] or Scer\GAL4[Hml.PG]) reduces melanization after Salmonella typhimurium infection. Knockdown of egr in the fat body or hemocytes of Salmonella typhimurium infected flies(through expression of egr[dsRNA.Scer\UAS] under the control of Scer\GAL4[Lsp2.PH] or Scer\GAL4[Hml.PG]) reduces feeding rates compared to infected wild-type flies. This change in feeding rate may be responsible for some aspects of the egr phenotype, although it must be noted that even in the absence of infection, egr knockdown flies consume less food. (Mabery and Schneider, 2010) Expression of egr^GS9830 under the control of Scer\GAL4^GMR.PF results in ablation of the adult eye. Co-expression of egr^IR.Scer\UAS under the control of Scer\GAL4^GMR.PF suppresses this phenotype. (Polaski et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Scer\GAL4^Act.PU/egr^{dsRNA.Scer\UAS} is an enhancer of partially lethal - majority die \| somatic clone \| pupal stage phenotype of scrib¹ (Ohsawa et al., 2011)
Suppressor of
Statement Reference egr^{dsRNA.Scer\UAS}/Scer\GAL4^egr-GAL4 is a suppressor \| partially of neuroanatomy defective phenotype of Ank2^f02001 (Keller et al., 2011) egr^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF is a suppressor \| partially of visible phenotype of Ras85D^KP (Wu et al., 2009)
NOT Suppressor of
Statement Reference egr^{dsRNA.Scer\UAS}, Scer\GAL4^dpp.blk1 is a non-suppressor of visible phenotype of HIV-1\Vpu^Scer\UAS.cLa, Scer\GAL4^dpp.blk1 (Marchal et al., 2012)
Phenotype Manifest In
Suppressor of
Statement Reference egr^{dsRNA.Scer\UAS}/Scer\GAL4^egr-GAL4 is a suppressor \| partially of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of Ank2^f02001 (Keller et al., 2011) egr^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF is a suppressor \| partially of eye phenotype of Ras85D^KP (Wu et al., 2009)
NOT Suppressor of
Statement Reference egr^{dsRNA.Scer\UAS}, Scer\GAL4^dpp.blk1 is a non-suppressor of wing phenotype of HIV-1\Vpu^Scer\UAS.cLa, Scer\GAL4^dpp.blk1 (Marchal et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Expression of egr[dsRNA.Scer\UAS] under the control of Scer\GAL4[egr-GAL4] partially suppresses the NMJ degeneration seen in Ank2[f02001] mutant third instar larvae. (Keller et al., 2011) Expression of egr[IR.Scer\UAS] under the control of Scer\GAL4[Act.PU] only within scrib[1] clones in the eye-antennal disc results in 30.7% pupal lethality, while expression of egr[IR.Scer\UAS] under the control of Scer\GAL4[Act.PU] both within scrib[1] clones in the eye-imaginal disc and in the surrounding wild-type cells results in 51.3% pupal lethality. (Ohsawa et al., 2011) The eye phenotype in Ras85D[KP] mutant flies is partially suppressed by ectopic expression of egr[IR.Scer\UAS] in the eye under the control of Scer\GAL4[GMR.PF]. (Wu et al., 2009)
Xenogenetic Interactions
Statement Reference Co-expression of egr[dsRNA.Scer\UAS] does not suppress the wing defects caused by expression of HIV-1\Vpu[Scer\UAS.cLa] under the control of Scer\GAL4[dpp.blk1]. (Marchal et al., 2012)
Complementation & Rescue Data
Rescues	Scer\GAL4^GMR.PF, egr^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF rescues egr^GS9830 (Igaki et al., 2002)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	egr^{dsRNA.Scer\UAS} egr^IR.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Marchal et al., 2012, PLoS ONE 7(3): e34310 The HIV-1 Vpu Protein Induces Apoptosis in Drosophila via Activation of JNK Signaling. [FBrf0218002] Keller et al., 2011, Neuron 72(5): 760--775 Glial-derived prodegenerative signaling in the Drosophila neuromuscular system. [FBrf0216904] Ohsawa et al., 2011, Dev. Cell 20(3): 315--328 Elimination of Oncogenic Neighbors by JNK-Mediated Engulfment in Drosophila. [FBrf0213219] Mabery and Schneider, 2010, J. Innate Immun. 2(4): 371--378 The Drosophila TNF ortholog eiger is required in the fat body for a robust immune response. [FBrf0211077] Neisch et al., 2010, J. Cell Biol. 189(2): 311--323 Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. [FBrf0210554] Wu et al., 2009, BMC Dev. Biol. 9: 53 Ras promotes cell survival by antagonizing both JNK and Hid signals in the Drosophila eye. [FBrf0209242] Polaski et al., 2006, Genetics 174(2): 719--733 Genetic analysis of slipper/mixed lineage kinase reveals requirements in multiple Jun-N-terminal kinase-dependent morphogenetic events during Drosophila development. [FBrf0193269] Igaki et al., 2002, EMBO J. 21(12): 3009--3018 Eiger, a TNF superfamily ligand that triggers the Drosophila JNK pathway. [FBrf0148977]

Allele Dmel\egrdsRNA.Scer\UAS

Allele Dmel\egr^{dsRNA.Scer\UAS}