FlyBase Allele Report: Dmel\c(2)M[Z0810]

General Information

Symbol

Dmel\c(2)M^Z0810

Species

D. melanogaster

Name

FlyBase ID

FBal0147243

Feature type

allele

Associated gene

Dmel\c(2)M

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Manheim and McKim, 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q457term.

(McKim, 2005.6.16)

FlyBase curator comment: Authors state lesion to be 'Amino acid replacement: Q436K'. However see McKim, 2005.6.16, personal communication to FlyBase for subsequent correction of this.

(Manheim and McKim, 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:16,305,020..16,305,020 [+]

Nucleotide change:

C16305020T

Reported nucleotide change:

C?T

Amino acid change:

Q457term | c(2)M-PA

Reported amino acid change:

Q457term

(Manheim and McKim, 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Bhagat et al., 2004, Manheim and McKim, 2003)

Phenotype Manifest In

oocyte (with c(2)M^EP2115)

(Gyuricza et al., 2016)

synaptonemal complex | adult stage | female (with c(2)M^EP2115)

(Gyuricza et al., 2016)

Detailed Description

Statement

Reference

Oocytes of c(2)M^EP2115/c(2)M^Z0810 transheterozygous mutants display defects in synaptonemal complex assembly, while the centromere clustering and sister chromatid cohesion (assessed by number of cid foci) in pachytene oocytes is not affected.

(Gyuricza et al., 2016)

c(2)M^Z0810/c(2)M^Z0810 female meioses exhibit reduced crossing over along the X chromosome.

(Bhagat et al., 2004)

Homozygotes exhibit a high rate on non-disjunction (19.8% X chromosome non-disjunction) and a reduced number of crossovers, with the number of crossovers per physical distance diminishing as the region approaches the centromere. Sister chromatid nondisjunction is seen in the offspring of homozygous c(2)M^Z0810 mothers at a frequency of 3.1% (10.5% of all non-disjunction events) when crossed with an X chromosome balancer, 1.5% (7.6%) respectively when not crossed with a balancer.

(Manheim and McKim, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

c(2)M^Z0810/c(2)M^Z0810 is an enhancer of abnormal meiotic cell cycle phenotype of okr⁷⁸²

(Bhagat et al., 2004)

NOT Suppressor of

Statement

Reference

c(2)M^Z0810 is a non-suppressor of sterile phenotype of okr^WS

(Manheim and McKim, 2003)

Other

Statement

Reference

c(2)M^Z0810, c(3)G⁶⁸ has abnormal meiotic cell cycle phenotype

(Manheim and McKim, 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

c(2)M^Z0810/c(2)M^EP2115 is a suppressor of oocyte phenotype of Nipped-B^GL00574

(Gyuricza et al., 2016)

c(2)M^Z0810/c(2)M^EP2115 is a suppressor of synaptonemal complex | female | adult stage phenotype of Nipped-B^GL00574

(Gyuricza et al., 2016)

NOT Suppressor of

Statement

Reference

c(2)M^Z0810/c(2)M^EP2115 is a non-suppressor of oocyte phenotype of SA1^GL00534

(Gyuricza et al., 2016)

c(2)M^Z0810/c(2)M^EP2115 is a non-suppressor of synaptonemal complex | female | adult stage phenotype of SA1^GL00534

(Gyuricza et al., 2016)

Other

Statement

Reference

c(2)M^Z0810/c(2)M^EP2115, ord⁵/ord¹⁰ has oocyte phenotype

(Gyuricza et al., 2016)

c(2)M^Z0810/c(2)M^EP2115, ord⁵/ord¹⁰ has chromosome | female | adult stage phenotype

(Gyuricza et al., 2016)

Df(3L)ED4470/sunn^167-109, c(2)M^Z0810/c(2)M^EP2115 has ovary phenotype

(Gyuricza et al., 2016)

Df(3L)ED4470/sunn^167-109, c(2)M^Z0810/c(2)M^EP2115 has synaptonemal complex | female | adult stage phenotype

(Gyuricza et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Combination with c(2)M^EP2115/c(2)M^Z0810 in transheterozygous state cannot suppress the decrease in average number of c(3)G-positive patches in oocytes between regions 2a and 3 of the germarium observed in flies expressing SA^GL00534 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} but it does ameliorate the reduction in patches number observed in flies expressing Nipped-B^GL00574.

sunn^167-109/Df(3L)ED4470;c(2)M^EP2115/c(2)M^Z0810 double mutant flies do not show any evidence of synaptonemal complex assembly in oocytes.

c(2)M^EP2115/c(2)M^Z0810,ord⁵/ord¹⁰ double mutants show defects in centromere clustering and sister chromatid cohesion (assessed by number of cid foci) in pachytene oocytes relative to controls.

(Gyuricza et al., 2016)

okr⁷⁸²/okr⁷⁸², c(2)M^Z0810/c(2)M^Z0810 double mutant female meioses exhibit reduced crossover frequency along most of the X chromosome, but significantly higher crossover frequency near the centromere, as compared to either single mutant.

(Bhagat et al., 2004)

c(3)G⁶⁸; c(2)M^Z0810 double mutant females exhibit crossovers at a higher than expected frequency.

(Manheim and McKim, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

c(2)M^EP2115

(Manheim and McKim, 2003)

Rescued by

c(2)M^Z0810/c(2)M^EP2115 is rescued by Scer\GAL4^{VP16.nanos.UTR}/c(2)M^UASp.Tag:HA

(Gyuricza et al., 2016)

c(2)M^Z0810 is rescued by Scer\GAL4^{VP16.nanos.UTR}/c(2)M^UASp.Tag:HA

(Manheim and McKim, 2003)

Partially rescued by

c(2)M^Z0810/c(2)M^EP2115 is partially rescued by c(2)M^UASp.Tag:HA/Scer\GAL4^hs.PB

(Gyuricza et al., 2016)

Not rescued by

c(2)M^Z0810/c(2)M^EP2115 is not rescued by c(2)M^UASp.Tag:HA/Scer\GAL4^VP16.bam

(Gyuricza et al., 2016)

Comments

A 1hr heat-shock induced pulse of expression of c(2)M^{Scer\UAS.P\T.T:Ivir\HA1} (controlled by Scer\GAL4^hs.PB) partially reduced the chromosome nondisjunction frequency in the eggs laid 7-10 days after the heat-shock (these would have been in the germarium at the time of the heat-shock), Scer\GAL4^{bam.T:Hsim\VP16}-driven expression of c(2)M^{Scer\UAS.P\T.T:Ivir\HA1} fails to rescue the nondisjunction phenotype, in contrast to expression driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} which results in full rescue.

(Gyuricza et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Phenotypic analysis (non-disjunction) provides an allelic series: c(2)M^EP2115 > c(2)M^Z0810 > c(2)M^Z3780.

(Manheim and McKim, 2003)

"Amino acid replacement: Q457@." was stated as revision.

(McKim, 2005.6.16)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

c(2)M^Z0810

(Gyuricza et al., 2016, Bhagat et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (4)

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