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General Information
Symbol
Dmel\mir-banC.UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0147279
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-bantam, UAS-bantam-GFP, UAS-C, UAS-ban
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Consists of a 584nt HpaI/SpeI fragment that includes the ban gene that has been cloned into the 3' UTR of Avic\GFPEGFP.UAS.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Third instar larval eye-antennal disc clones expressing banC.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Act5C.PI exhibit reduced epithelial folding and abnormal mixing of cells across the field boundary at the A1 fold, as compared to controls.

Larval eye-antennal discs expressing banC.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.blk1 exhibit decreased cell proliferation, but no significant change in apoptosis levels, as compared to controls.

Expression of banC.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4esg-NP5130 increases the number of midgut precursor cells. An increased number of intestinal stem cells are undergoing DNA replication compared to controls.

Expression of banC.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4esg-NP5130 (restricted to the adult stages using Scer\GAL80ts.αTub84B) results in an increase in midgut precursor cells compared to controls. An increase in cell proliferation (pH3 staining) is observed.

Intestinal stem cell differentiation is unaffected in clones expressing banC.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL80ts.αTub84B, but the clones exhibit a growth advantage over control clones.

Expressing mir-banC.UAS.EGFP under the control of Scer\GAL4ap-md544 (in combination with a Gal80[ts] transgene to inhibit expression until early third instar) results in severely overgrown wing discs in the dorsal compartment, but with an epithelial organization similar to wild-type controls in third instar larvae. There is no effect on cell death.

Late third instar wing discs expressing banC.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4sd-SG29.1 have greatly disturbed dorsal-ventral (DV) boundaries compared to controls. The anterior-posterior boundary remains straight and smooth. BrdU staining shows that increased boundary cell proliferation is occurring at the DV boundary. This uneven DV boundary phenotype is also observed in the wing discs of mid-third instar larvae, and when banC.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4vg.int2.1.

When banC.Scer\UAS.T:Avic\GFP-EGFP is driven by Scer\GAL4en-e16E overgrowth is seen in the posterior compartment of the wing and of segments in the larval body. banC.Scer\UAS.T:Avic\GFP-EGFP when driven by Scer\GAL4ptc-559.1 is sufficient to direct cells in the non-proliferating zone of the wing disc to enter S phase.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expressing EgfrUAS.cBa and mir-banC.UAS.EGFP under the control of Scer\GAL4ap-md544 (in combination with a Gal80[ts] transgene to inhibit expression until early third instar) results in severely overgrown and deformed wing discs with a disrupted epithelial organization (resembling tissues undergoing a neoplastic transformation) in third instar larvae.

Expression of dmGD1419 is unable to suppress the dorsal-ventral boundary defects seen when banC.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4sd-SG29.1.

Expression of trblScer\UAS.cMa is unable to suppress the dorsal-ventral boundary defects seen when banC.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4sd-SG29.1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The addition of banC.Scer\UAS.T:Avic\GFP-EGFP (driven by Scer\GAL4arm.PS) to Df(3L)banΔ1 animals, rescues the growth defect in the imaginal discs and leads to morphologically normal pupae, many of which survive to adulthood.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
banC.Scer\UAS.T:Avic\GFP-EGFP
banC.UAS.EGFP
mir-banC.UAS.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (12)