FlyBase Allele Report: Dmel\mir-ban[C.UAS.EGFP]

General Information

Symbol

Dmel\mir-ban^C.UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0147279

Feature type

allele

Associated gene

Dmel\mir-ban

Associated Insertion(s)

Carried in Construct

P{UAS-EGFP-mir-ban.C}

Also Known As

UAS-bantam, UAS-bantam-GFP, UAS-C, UAS-ban

Key Links

Transgenic product class

wild_type

(Brennecke et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Brennecke et al., 2003)

Mutagen

in vitro construct

(Brennecke et al., 2003)

Progenitor genotype

Avic\GFP^EGFP.cSa.UAS

(Brennecke et al., 2003)

Carried in construct

P{UAS-EGFP-mir-ban.C}

Cytology

Description

Consists of a 584nt HpaI/SpeI fragment that includes the ban gene that has been cloned into the 3' UTR of Avic\GFP^EGFP.UAS.

(Brennecke et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-ban

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of carcinoma

CEA with Egfr^UAS.cBa

(Herranz et al., 2012)

model of cancer

CEA with Rab5^LL00467

(Cong et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Dubey and Tapadia, 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cell cycle, with Scer\GAL4^ptc-559.1

(Brennecke et al., 2003)

dorsal-ventral compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^sd-SG29.1

(Becam et al., 2011)

dorsal-ventral compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^vg.int2.1

(Becam et al., 2011)

eye-antennal disc | larval stage, with Scer\GAL4^dpp.blk1

(Ku and Sun, 2017)

eye-antennal disc | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PI

(Ku and Sun, 2017)

larva, with Scer\GAL4^en-e16E

(Brennecke et al., 2003)

larval segment, with Scer\GAL4^en-e16E

(Brennecke et al., 2003)

ommatidium | adult stage, with Scer\GAL4^ey.PH

(Brittle et al., 2012)

wing disc | larval stage, with Scer\GAL4^ptc-559.1

(Brennecke et al., 2003)

wing disc dorsal compartment | third instar larval stage, with Scer\GAL4^ap-md544

(Herranz et al., 2012)

wing disc posterior compartment | larval stage, with Scer\GAL4^en-e16E

(Brennecke et al., 2003)

Detailed Description

Statement

Reference

Third instar larval eye-antennal disc clones expressing ban^{C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act5C.PI exhibit reduced epithelial folding and abnormal mixing of cells across the field boundary at the A1 fold, as compared to controls.

Larval eye-antennal discs expressing ban^{C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^dpp.blk1 exhibit decreased cell proliferation, but no significant change in apoptosis levels, as compared to controls.

(Ku and Sun, 2017)

Expression of ban^{C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^esg-NP5130 increases the number of midgut precursor cells. An increased number of intestinal stem cells are undergoing DNA replication compared to controls.

Expression of ban^{C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^esg-NP5130 (restricted to the adult stages using Scer\GAL80^ts.αTub84B) results in an increase in midgut precursor cells compared to controls. An increase in cell proliferation (pH3 staining) is observed.

Intestinal stem cell differentiation is unaffected in clones expressing ban^{C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL80^ts.αTub84B, but the clones exhibit a growth advantage over control clones.

(Huang et al., 2014)

Expressing mir-ban^C.UAS.EGFP under the control of Scer\GAL4^ap-md544 (in combination with a Gal80[ts] transgene to inhibit expression until early third instar) results in severely overgrown wing discs in the dorsal compartment, but with an epithelial organization similar to wild-type controls in third instar larvae. There is no effect on cell death.

(Herranz et al., 2012)

Late third instar wing discs expressing ban^{C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sd-SG29.1 have greatly disturbed dorsal-ventral (DV) boundaries compared to controls. The anterior-posterior boundary remains straight and smooth. BrdU staining shows that increased boundary cell proliferation is occurring at the DV boundary. This uneven DV boundary phenotype is also observed in the wing discs of mid-third instar larvae, and when ban^{C.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^vg.int2.1.

(Becam et al., 2011)

When ban^{C.Scer\UAS.T:Avic\GFP-EGFP} is driven by Scer\GAL4^en-e16E overgrowth is seen in the posterior compartment of the wing and of segments in the larval body. ban^{C.Scer\UAS.T:Avic\GFP-EGFP} when driven by Scer\GAL4^ptc-559.1 is sufficient to direct cells in the non-proliferating zone of the wing disc to enter S phase.

(Brennecke et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, mir-ban^C.UAS.EGFP has abnormal size | third instar larval stage phenotype, enhanceable by Egfr^UAS.cBa, Scer\GAL4^ap-md544

(Herranz et al., 2012)

Enhancer of

Statement

Reference

mir-ban^C.UAS.EGFP, Scer\GAL4^ap-md544 is an enhancer of abnormal size | third instar larval stage phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544

(Herranz et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^sca.PU/mir-ban^C.UAS.EGFP is a non-suppressor of visible phenotype of Scer\GAL4^sca.PU, mi^GD11624

(Szuplewski et al., 2012)

Other

Statement

Reference

Rab5^LL00467, Scer\GAL4^Act5C.PI, mir-ban^C.UAS.EGFP has neoplasia | somatic clone | larval stage phenotype

(Cong et al., 2021)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, mir-ban^C.UAS.EGFP has neoplasia | third instar larval stage phenotype

(Herranz et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, mir-ban^C.UAS.EGFP has wing disc dorsal compartment | third instar larval stage phenotype, enhanceable by Egfr^UAS.cBa, Scer\GAL4^ap-md544

(Herranz et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^sd-SG29.1, mir-ban^C.UAS.EGFP has dorsal-ventral compartment boundary of the wing disc | third instar larval stage phenotype, non-suppressible by Myc^GD1419, Scer\GAL4^sd-SG29.1

(Becam et al., 2011)

Scer\GAL4^sd-SG29.1, mir-ban^C.UAS.EGFP has dorsal-ventral compartment boundary of the wing disc | third instar larval stage phenotype, non-suppressible by Scer\GAL4^sd-SG29.1/trbl^UAS.cMa

(Becam et al., 2011)

Enhancer of

Statement

Reference

mir-ban^C.UAS.EGFP, Scer\GAL4^ap-md544 is an enhancer of wing disc dorsal compartment | third instar larval stage phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544

(Herranz et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^sca.PU/mir-ban^C.UAS.EGFP is a non-suppressor of macrochaeta phenotype of Scer\GAL4^sca.PU, mi^GD11624

(Szuplewski et al., 2012)

Other

Statement

Reference

Rab5^LL00467, Scer\GAL4^Act5C.PI, mir-ban^C.UAS.EGFP has larval ventral nerve cord | somatic clone phenotype

(Cong et al., 2021)

Rab5^LL00467, Scer\GAL4^Act5C.PI, mir-ban^C.UAS.EGFP has larval brain | somatic clone | larval stage phenotype

(Cong et al., 2021)

Rab5^LL00467, Scer\GAL4^Act5C.PI, mir-ban^C.UAS.EGFP has eye-antennal disc | somatic clone | larval stage phenotype

(Cong et al., 2021)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, mir-ban^C.UAS.EGFP has wing disc | third instar larval stage phenotype

(Herranz et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Clones both homozygous for Rab5^LL00467 and expressing mir-ban^C.UAS.EGFP under the control of Scer\GAL4^Act5C.PI form tumors in the larval brain (which metastasize to the ventral nerve cord). Larval eye/antennal disc clones also form tumors and their induction leads to lethality. Co-expression of JhI-21^HMS02271 suppresses tumorigenesis and lethality, but adults exhibit an eye phenotype.

(Cong et al., 2021)

Co-expressing Egfr^UAS.cBa and mir-ban^C.UAS.EGFP under the control of Scer\GAL4^ap-md544 (in combination with a Gal80[ts] transgene to inhibit expression until early third instar) results in severely overgrown and deformed wing discs with a disrupted epithelial organization (resembling tissues undergoing a neoplastic transformation) in third instar larvae.

(Herranz et al., 2012)

Expression of dm^GD1419 is unable to suppress the dorsal-ventral boundary defects seen when ban^{C.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^sd-SG29.1.

Expression of trbl^Scer\UAS.cMa is unable to suppress the dorsal-ventral boundary defects seen when ban^{C.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^sd-SG29.1.

(Becam et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

mir-ban^C.UAS.EGFP/Scer\GAL4^arm.PS rescues Df(3L)mir-ban^Δ1

(Brennecke et al., 2003)

Comments

The addition of ban^{C.Scer\UAS.T:Avic\GFP-EGFP} (driven by Scer\GAL4^arm.PS) to Df(3L)ban^Δ1 animals, rescues the growth defect in the imaginal discs and leads to morphologically normal pupae, many of which survive to adulthood.

(Brennecke et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

60672

w^*; P{UAS-EGFP-mir-ban.C}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ban^{C.Scer\UAS.T:Avic\GFP-EGFP}

ban^C.UAS.EGFP

mir-ban^C.UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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