Third instar larval eye-antennal disc clones expressing banC.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Act5C.PI exhibit reduced epithelial folding and abnormal mixing of cells across the field boundary at the A1 fold, as compared to controls.
Larval eye-antennal discs expressing banC.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.blk1 exhibit decreased cell proliferation, but no significant change in apoptosis levels, as compared to controls.
Expressing mir-banC.UAS.EGFP under the control of Scer\GAL4ap-md544 (in combination with a Gal80[ts] transgene to inhibit expression until early third instar) results in severely overgrown wing discs in the dorsal compartment, but with an epithelial organization similar to wild-type controls in third instar larvae. There is no effect on cell death.
Late third instar wing discs expressing banC.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4sd-SG29.1 have greatly disturbed dorsal-ventral (DV) boundaries compared to controls. The anterior-posterior boundary remains straight and smooth. BrdU staining shows that increased boundary cell proliferation is occurring at the DV boundary. This uneven DV boundary phenotype is also observed in the wing discs of mid-third instar larvae, and when banC.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4vg.int2.1.