Allele Tni\Tni\piggyBac\T^αTub84B.PH

General Information
Symbol	Tni\Tni\piggyBac\T^αTub84B.PH	Species	T. ni
Name	α-Tubulin at 84B promoter construct of Horn	FlyBase ID	FBal0147355
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Tni\Tni\piggyBac\T

Allele class
Mutagen	in vitro construct \| regulatory fusion, in vitro construct \| coding region fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Horn et al., 2003, Hacker et al., 2003) in vitro construct \| coding region fusion (Hacker et al., 2003)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: An αTub84B promoter drives expression of Tni\piggyBac\T. (Horn et al., 2003) The Tni\piggyBac\T transposase coding region with the 3' UTR from fs(1)K10 under the control of the αTub84B promoter was cloned into P{Express-FRT.2.2}. (Parks, 2004.5.12)
Assay mode	In transgenic Drosophila (allele of foreign species in genome of drosophilid) (Hacker et al., 2003, Horn et al., 2003)
Carried in construct	P{FRT(w⁺)Tub-PBac\T} (Parks, 2004.5.12, Thibault et al., 2004) P{Tub-PBac\T} (Parks, 2004.5.12, Thibault et al., 2004) Herm{3xP3-ECFP,αtub-piggyBacK10} (Horn et al., 2003, Hacker et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	8285 w¹¹¹⁸; CyO, P{Tub-PBac\T}2/wg^Sp-1 8283 w¹¹¹⁸; CyO, P{FRT(w⁺)Tub-PBac\T}2/wg^Sp-1
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Tni\piggyBac\T^αTub84B.PH
Name Synonym	α-Tubulin at 84B promoter construct of Horn (Horn et al., 2003)
Secondary FlyBase IDs

References ( 4 )
Research paper	Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002] Hacker et al., 2003, Proc. Natl. Acad. Sci. USA 100(13): 7720--7725 piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila. [FBrf0159706] Horn et al., 2003, Genetics 163(2): 647--661 piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. [FBrf0158985]
Personal communication to FlyBase	Parks, 2004.5.12, Flip-out piggyBac transposase constructs and insertions. Flip-out piggyBac transposase constructs and insertions. [FBrf0178854]

Allele Tni\Tni\piggyBac\TαTub84B.PH

Allele Tni\Tni\piggyBac\T^αTub84B.PH