FlyBase Allele Report: Dmel\Thor[UAS.cMa]

General Information

Symbol

Dmel\Thor^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Miron

FlyBase ID

FBal0147361

Feature type

allele

Associated gene

Dmel\Thor

Associated Insertion(s)

Carried in Construct

P{UAS-Thor.wt}

Also Known As

UAS-4E-BP, UAS-Thor, UAS-4eBP, UAS-4E-BP^wt

Key Links

Transgenic product class

wild_type

(Miron et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Miron et al., 2001)

Mutagen

in vitro construct

(Miron et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Thor.wt}

Cytology

Description

UASt regulatory sequences drive expression of wild-type Thor.

(Miron et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Thor

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Chchd2^H43

(Meng et al., 2017)

modeled by Pink1^B9

(Koh et al., 2012, Tain et al., 2009)

modeled by park²⁵

(Tain et al., 2009)

ameliorates congenital muscular dystrophy due to LMNA mutation

modeled by LamC^G489V.UAS

(Chandran et al., 2019)

ameliorates Parkinson's disease 6

modeled by Pink1^RNAi.UAS.cYa

(Liu and Lu, 2010)

modeled by Pink1^B9

(Liu and Lu, 2010)

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Mhc.PW

(Lee et al., 2010)

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Mushtaq et al., 2022)

chemical resistant, with Scer\GAL4^da.G32

(Imai et al., 2008)

short lived, with Scer\GAL4^elav.PU

(Li et al., 2023)

Phenotype Manifest In

NMJ bouton | decreased number | larval stage, with Scer\GAL4^elav-C155

(Mushtaq et al., 2022)

type I bouton, with Scer\GAL4^Mhc.PW

(Lee et al., 2010)

Detailed Description

Statement

Reference

The expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^da.PU does not induce mitochondria morphology defects in adult indirect flight muscles, as compared to controls; expression under the control of Scer\GAL4^Ddc.PU does not induce significant defects in the adult life-span, in the adult locomotor capacity or in the number of dopaminergic PAL, PPM1/2, PPM3, PPL1 or PPL2 neurons in the adult brain, as compared to controls.

(Meng et al., 2017)

Ectopic expression of Thor^Scer\UAS.cMa under the Scer\GAL4^elav-C155 driver does not alter the number of neuromuscular junction boutons in third instar larvae compared to controls.

(Wong et al., 2015)

Expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^Lsp2.PH rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet.

Expression of Thor^Scer\UAS.cMa under the simultaneous control of both Scer\GAL4^Scer\UAS.cHa and Scer\GAL4^tin.cBa does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals.

(Birse et al., 2010)

Post-synaptic expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^Mhc.PW leads to a decrease in the total number of type I boutons (both type Ib and type Is) on muscle 6/7 of A3. Presynaptic expression of Thor^Scer\UAS.cMa (under the control of Scer\GAL4^elav-C155) has no effect.

(Lee et al., 2010)

Overexpression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^arm.PS does not change lifespan on rich food in males or females. Under dietary restriction, there is no lifespan extension in these flies

(Zid et al., 2009)

Flies expressing Thor^Scer\UAS.cMa under the control of Scer\GAL4^da.G32 are more resistant to hydrogen peroxide compared to control flies.

(Imai et al., 2008)

Overexpression of Thor^Scer\UAS.cMa, under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} has no effect on fat body internalisation of TR-avidin.

(Hennig et al., 2006)

Expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^Bx-MS1096 has no effect on wing size or pattern. Expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF does not result in any discernible phenotype in the eye.

(Miron et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, Thor^UAS.cMa has abnormal neuroanatomy | larval stage phenotype, suppressible by Madm^UAS.EGFP, Scer\GAL4^elav-C155

(Mushtaq et al., 2022)

Enhancer of

Statement

Reference

Thor^UAS.cMa, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Hsap\PRNP^V129.UAS, Scer\GAL4^GMR.PU

(Myers et al., 2022)

Thor^UAS.cMa, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Suppressor of

Statement

Reference

Thor^UAS.cMa, Scer\GAL4^elav.PU is a suppressor | partially of decreased rate of adult locomotory behavior | progressive phenotype of Ddrgk1^NIG.5862R, Scer\GAL4^elav.PU

(Li et al., 2023)

Thor^UAS.cMa, Scer\GAL4^elav.PU is a suppressor | partially of short lived phenotype of Ddrgk1^NIG.5862R, Scer\GAL4^elav.PU

(Li et al., 2023)

Thor^UAS.cMa, Scer\GAL4^fln.IFM is a suppressor of flightless | progressive phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Scer\GAL4^Ddc.PU/Thor^UAS.cMa is a suppressor of short lived phenotype of Chchd2^H43

(Meng et al., 2017)

Scer\GAL4^Ddc.PU/Thor^UAS.cMa is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Chchd2^H43

(Meng et al., 2017)

Thor^UAS.cMa/Scer\GAL4^hs.PU is a suppressor | partially of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

Scer\GAL4^ple.PF/Thor^UAS.cMa is a suppressor of decreased cell number | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

Thor^UAS.cMa, Scer\GAL4^Mhc.PW is a suppressor of abnormal neuroanatomy phenotype of Lrrk^UAS.cIa, Scer\GAL4^Mhc.PW

(Lee et al., 2010)

Scer\GAL4^da.PU/Thor^UAS.cMa is a suppressor of partially lethal - majority die phenotype of Thor², park²⁵

(Tain et al., 2009)

Scer\GAL4^da.PU/Thor^UAS.cMa is a suppressor of partially lethal - majority live phenotype of Pink1^B9, Thor²

(Tain et al., 2009)

Scer\GAL4^how-24B/Thor^UAS.cMa is a suppressor | partially of abnormal flight | recessive phenotype of Pink1^B9

(Tain et al., 2009)

Scer\GAL4^how-24B/Thor^UAS.cMa is a suppressor | partially of abnormal locomotor behavior | recessive | adult stage phenotype of Pink1^B9

(Tain et al., 2009)

Scer\GAL4^ple.PF/Thor^UAS.cMa is a suppressor of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Tain et al., 2009)

Scer\GAL4^how-24B/Thor^UAS.cMa is a suppressor of abnormal flight | recessive phenotype of park²⁵

(Tain et al., 2009)

Scer\GAL4^how-24B/Thor^UAS.cMa is a suppressor of abnormal locomotor behavior | recessive | adult stage phenotype of park²⁵

(Tain et al., 2009)

Scer\GAL4^ple.PF/Thor^UAS.cMa is a suppressor of abnormal neuroanatomy | adult stage phenotype of park²⁵

(Tain et al., 2009)

Thor^UAS.cMa, Scer\GAL4^ple.PF is a suppressor of abnormal neuroanatomy | adult stage phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Imai et al., 2008)

Thor^UAS.cMa, Scer\GAL4^ple.PF is a suppressor of abnormal neuroanatomy | adult stage phenotype of Lrrk^Y1383C.UAS, Scer\GAL4^ple.PF

(Imai et al., 2008)

Thor^UAS.cMa, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Miron et al., 2001)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ey-OK107/Thor^UAS.cMa is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage | progressive phenotype of bsk^flp147E

(Rallis et al., 2013)

Scer\GAL4^elav-C155/Thor^UAS.cMa is a non-suppressor of abnormal neuroanatomy phenotype of Lrrk^UAS.cIa, Scer\GAL4^Mhc.PW

(Lee et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, Thor^UAS.cMa has NMJ bouton | larval stage | decreased number phenotype, suppressible by Madm^UAS.EGFP, Scer\GAL4^elav-C155

(Mushtaq et al., 2022)

Enhancer of

Statement

Reference

Thor^UAS.cMa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\PRNP^V129.UAS, Scer\GAL4^GMR.PU

(Myers et al., 2022)

Thor^UAS.cMa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Suppressor of

Statement

Reference

Thor^UAS.cMa, Scer\GAL4^fln.IFM is a suppressor of indirect flight muscle cell | adult stage phenotype of LamC^R205W.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Thor^UAS.cMa, Scer\GAL4^fln.IFM is a suppressor of wing phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Thor^UAS.cMa, Scer\GAL4^fln.IFM is a suppressor of indirect flight muscle cell | adult stage phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Thor^UAS.cMa, Scer\GAL4^fln.IFM is a suppressor of nucleus | adult stage phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Thor^UAS.cMa, Scer\GAL4^fln.IFM is a suppressor of myofibril | adult stage phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Thor^UAS.cMa, Scer\GAL4^fln.IFM is a suppressor of mitochondrion | adult stage phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Scer\GAL4^Ddc.PU/Thor^UAS.cMa is a suppressor of dopaminergic PPL1 neuron | adult stage | progressive phenotype of Chchd2^H43

(Meng et al., 2017)

Scer\GAL4^da.PU/Thor^UAS.cMa is a suppressor of mitochondrion | adult stage | progressive phenotype of Chchd2^null

(Meng et al., 2017)

Scer\GAL4^da.PU/Thor^UAS.cMa is a suppressor of mitochondrial crista | adult stage | progressive phenotype of Chchd2^null

(Meng et al., 2017)

Thor^UAS.cMa/Scer\GAL4^hs.PU is a suppressor | partially of adult thorax phenotype of Pink1^B9

(Koh et al., 2012)

Thor^UAS.cMa/Scer\GAL4^hs.PU is a suppressor | partially of mitochondrion | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

Thor^UAS.cMa/Scer\GAL4^hs.PU is a suppressor | partially of indirect flight muscle cell phenotype of Pink1^B9

(Koh et al., 2012)

Thor^UAS.cMa/Scer\GAL4^hs.PU is a suppressor | partially of wing blade phenotype of Pink1^B9

(Koh et al., 2012)

Scer\GAL4^ple.PF/Thor^UAS.cMa is a suppressor of dorso-lateral dopaminergic neuron phenotype of Pink1^B9

(Koh et al., 2012)

Thor^UAS.cMa, Scer\GAL4^Mhc.PW is a suppressor of neuromuscular junction phenotype of Lrrk^UAS.cIa, Scer\GAL4^Mhc.PW

(Lee et al., 2010)

Scer\GAL4^how-24B/Thor^UAS.cMa is a suppressor of mitochondrion phenotype of Pink1^B9

(Tain et al., 2009)

Scer\GAL4^how-24B/Thor^UAS.cMa is a suppressor of indirect flight muscle cell phenotype of Pink1^B9

(Tain et al., 2009)

Scer\GAL4^ple.PF/Thor^UAS.cMa is a suppressor of dopaminergic PPL1 neuron phenotype of Pink1^B9

(Tain et al., 2009)

Scer\GAL4^how-24B/Thor^UAS.cMa is a suppressor of mitochondrion phenotype of park²⁵

(Tain et al., 2009)

Scer\GAL4^how-24B/Thor^UAS.cMa is a suppressor of indirect flight muscle cell phenotype of park²⁵

(Tain et al., 2009)

Scer\GAL4^ple.PF/Thor^UAS.cMa is a suppressor of dopaminergic PPL1 neuron phenotype of park²⁵

(Tain et al., 2009)

Thor^UAS.cMa, Scer\GAL4^ple.PF is a suppressor of dopaminergic neuron phenotype of Lrrk^Y1383C.UAS, Scer\GAL4^ple.PF

(Imai et al., 2008)

Thor^UAS.cMa, Scer\GAL4^ple.PF is a suppressor of dopaminergic neuron phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Imai et al., 2008)

Thor^UAS.cMa, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Miron et al., 2001)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ey-OK107/Thor^UAS.cMa is a non-suppressor of alpha/beta Kenyon cell phenotype of bsk^flp147E

(Rallis et al., 2013)

Scer\GAL4^ey-OK107/Thor^UAS.cMa is a non-suppressor of gamma Kenyon cell phenotype of bsk^flp147E

(Rallis et al., 2013)

Scer\GAL4^Mhc.PW/Thor^UAS.cMa is a non-suppressor of neuromuscular junction phenotype of Lrrk^UAS.cIa, Scer\GAL4^elav-C155

(Lee et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of LamC^UAS.cSa and Thor^UAS.cMa under the control of Scer\GAL4^fln.IFM under the control of Scer\GAL4^fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

(Chandran et al., 2019)

S6k^l-1 homozygous somatic clones also expressing Thor^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL in third instar larval eye discs do not exhibit any delay in G1/S transition during the second mitotic wave, as mutant cells enter S phase in a similar region to control cells.

(Kim et al., 2017)

The expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^da.PU suppresses the frequency and severity of the mitochondria morphology defects displayed in Chchd2^H43 homozygous adult indirect flight muscles. Expression under the control of Scer\GAL4^Ddc.PU suppresses the decreased life-span, the locomotor defects and the decreased number of dopaminergic PPL1 neurons in the brain, presented by Chchd2^H43 homozygous adults; this expression, however, does not significantly affect the numbers of dopaminergic PAL, PPM1/2, PPM3 or PPL2 neurons in the adult brain of Chchd2^H43 homozygotes.

Expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^da.PU also suppresses the mitochondria morphology defects in adult indirect flight muscle exhibited by Chchd2^null homozygotes.

(Meng et al., 2017)

Expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^ey-OK107 fails to suppress the axon degeneration seen in bsk^flp147E mutant mushroom body neuron clones.

(Rallis et al., 2013)

Expression of Sod2^Scer\UAS.cUa or Thor^Scer\UAS.cMa driven by Scer\GAL4^hs.PU partially suppresses phenotypes (collapsed thorax, downturned wings, reduced levels of mtDNA and ATP in the indirect flight muscle, and locomotor defects) seen in Pink1^B9/Y flies. Expression of Sod2^Scer\UAS.cUa or Thor^Scer\UAS.cMa driven by Scer\GAL4^ple.PF partially suppresses the enlarged mitochondria in dopaminergic neurons and dopaminergic neuron loss (DL1 cluster) seen in Pink1^B9/Y flies.

(Koh et al., 2012)

The reduction of neuromuscular junctions caused by post-synaptic expression of Lrrk^Scer\UAS.cIa (under the control of Scer\GAL4^Mhc.PW) is fully suppresses by the coexpression of Thor^Scer\UAS.cMa.

Coexpression of Thor^Scer\UAS.cMa in the post-synaptic compartment under the control of Scer\GAL4^Mhc.PW is unable to rescue the neuromuscular junction phenotype caused by presynaptic Lrrk^Scer\UAS.cIa expression (under the control of Scer\GAL4^elav-C155.

(Lee et al., 2010)

Thor²/Thor²; park²⁵/park²⁵ double mutants are almost completely lethal and Thor²/Thor²; Pink1^B9/Pink1^B9 double mutants are partially lethal (unlike either viable single mutant or viable single mutants with the other allele heterozygotic), with rare escapers dying soon after eclosion; expression of Thor^Scer\UAS.cMa driven by Scer\GAL4^da.PU suppresses lethality in double mutants.

Expression of Thor^Scer\UAS.cMa driven by Scer\GAL4^how-24B significantly partially suppresses flight and climbing defects as well as muscle degeneration and mitochondrial disruption in Pink1^B9/Pink1^B9 flies. Expression of Thor^Scer\UAS.cMa driven by Scer\GAL4^ple.PF significantly suppresses loss of dopaminergic PPL1 neurons in 30 day old Pink1^B9/Pink1^B9 flies. Expression of foxo^Scer\UAS.cFa driven by Scer\GAL4^how-24B significantly partially suppresses flight and climbing defects as well as muscle degeneration and mitochondrial disruption in flies. Expression of Thor^Scer\UAS.cMa or foxo^Scer\UAS.cFa driven by Scer\GAL4^ple.PF significantly suppresses loss of dopaminergic PPL1 neurons in 30 day old park²⁵/park²⁵ flies.

(Tain et al., 2009)

The decrease in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster that is seen in 60 day old adults expressing Lrrk^{I1915T.Scer\UAS} under the control of Scer\GAL4^ple.PF is suppressed by co-expression of Thor^Scer\UAS.cMa.

The decrease in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster that is seen in 60 day old adults expressing Lrrk^{Y1383C.Scer\UAS} under the control of Scer\GAL4^ple.PF is suppressed by co-expression of Thor^Scer\UAS.cMa.

(Imai et al., 2008)

The enlarged eye phenotype caused by expression of Akt1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF is partially suppressed by co-expression of Thor^Scer\UAS.cMa.

(Miron et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^da.G32/Thor^UAS.cMa rescues Thor^k13517

(Zid et al., 2009)

Scer\GAL4^da.G32/Thor^UAS.cMa rescues Thor^k13517

(Imai et al., 2008)

Comments

Ubiquitous expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^da.G32 rescues the dietary restriction phenotype of Thor^k13517 mutants from 8% to 37% lifespan extension in males and from 13% to 48% in females, similar to controls.

(Zid et al., 2009)

The increased sensitivity of Thor^k13517 flies to paraquat and to hydrogen peroxide is rescued by expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^da.G32.

(Imai et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9147

w^*; P{UAS-Thor.wt}2

8649

w^*; wg^Sp-1/CyO; P{UAS-Thor.wt}3

Notes on Origin

Discoverer

Comments

Carried in a plasmid, transfected into S2 cells and used in translation assays.

(Ahmed and Duncan, 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Thor^Scer\UAS.cMa

Thor^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Miron

(Miron et al., 2001)

Secondary FlyBase IDs

References (24)

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