FlyBase Allele Report: Dmel\Rack1[EY00128]

General Information

Symbol

Dmel\Rack1^EY00128

Species

D. melanogaster

Name

FlyBase ID

FBal0147577

Feature type

allele

Associated gene

Dmel\Rack1

Associated Insertion(s)

P{EPgy2}Rack1^EY00128

Carried in Construct

Also Known As

Rack1^EY128

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Kadrmas et al., 2007)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}Rack1^EY00128

Cytology

Description

Carries a P-element 53bp upstream of the Rack1 translational start site.

(Kadrmas et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:7,827,220..7,827,220 [+]

(Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates muscular dystrophy

modeled by Dg³²³

(Kucherenko et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

(Kucherenko et al., 2011)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cuticle (with Df(2L)RACK1-mts)

(Kadrmas et al., 2007)

cuticle (with Rack1^1.8)

(Kadrmas et al., 2007)

egg (with Rack1^EE)

(Kadrmas et al., 2007)

female germline stem cell (with Rack1^EE)

(Kadrmas et al., 2007)

oocyte | somatic clone

(Kucherenko et al., 2008)

ovary (with Df(2L)RACK1-mts)

(Kadrmas et al., 2007)

ovary (with Rack1^1.8)

(Kadrmas et al., 2007)

ovary (with Rack1^EE)

(Kadrmas et al., 2007)

wing (with Df(2L)RACK1-mts)

(Kadrmas et al., 2007)

wing (with Rack1^1.8)

(Kadrmas et al., 2007)

Detailed Description

Statement

Reference

Rack1^EY00128/+ mutant flies exhibit temperature-induced mobility defects.

(Kucherenko et al., 2011)

Rack1^EY00128 mutant germline clones show oocyte mislocalisation.

(Kucherenko et al., 2008)

Although Rack1^EE/Rack1^EY00128 mutants show larval lethality, up to 35% of animals are escapers. Females at 3 days posteclosion contain ovaries that are reduced in size but have no gross morphological defects. At 7 days, the ovarioles are crowded with small, but mature, stage 14 eggs. Rack1^EE/Rack1^EY00128 germaria have reduced numbers of germline stem cells but these are never missing completely. The number of germ-line derived cells in the egg chambers of these mutants does not seem affected. Oogenesis can proceed relatively normally to stage 14, but the resulting eggs are not laid and accumulate in the ovariole.

Rack1^EY00128/Df(2L)RACK1-mts and Rack1^EY00128/Rack1^1.8 escapers display a range of phenotypes including sluggish movement, difficulty in exiting the pupal case, cuticle that does not harden, and wings that are blistered, abnormally small, or that fail to unfold. Escaper females are incapable of laying eggs and the size of the ovary is reduced compared to wild type but overall body size is unaffected.

(Kadrmas et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rack1[+]/Rack1^EY00128 is an enhancer of visible phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Suppressor of

Statement

Reference

Rack1[+]/Rack1^EY00128 is a suppressor of chemical sensitive | adult stage phenotype of Otud6^C183A

(Villa et al., 2024)

Rack1[+]/Rack1^EY00128 is a suppressor of increased mortality | adult stage phenotype of Otud6^C183A

(Villa et al., 2024)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rack1[+]/Rack1^EY00128 is an enhancer of posterior crossvein phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

NOT Enhancer of

Statement

Reference

Rack1[+]/Rack1^EY00128 is a non-enhancer of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Rack1[+]/Rack1^EY00128 is a non-enhancer of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Other

Statement

Reference

Df(3R)Exel6184/+, Rack1^EY00128 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg³²³, Rack1^EY00128 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Rack1 Dys double heterozygous flies (Rack1^EY00128/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

Rack1^EY00128 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

One copy of Rack1^EY00128 does not enhance the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of Rack1^EY00128 does not enhance the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

Rack1^EY00128 Dg³²³ double heterozygous flies exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

One copy of Rack1^EY00128 enhances the posterior crossvein phenotype seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

15000

y¹ w^67c23; P{EPgy2}Rack1^EY00128/CyO

Kyoto

111662

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{EPgy2}Rack1^EY00128 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Rack1^EP

(Villa et al., 2024)

Rack1^EY00128

(Kucherenko et al., 2011, Marrone et al., 2011)

Rack1^EY128

(Luo et al., 2019, Kadrmas et al., 2007)

Rack1[EY00128]

(Kucherenko et al., 2008)

rack1^EY00128

(Sun et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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