Allele Dmel\tum^{dsRNA.1110-1961}

General Information
Symbol	Dmel\tum^{dsRNA.1110-1961}	Species	D. melanogaster
Name		FlyBase ID	FBal0147578
Feature type	allele	Associated gene	Dmel\tum

Allele class
Mutagen	in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - RNAi (Somma et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Template corresponding to nucleotides 1110-1961 of RacGAP50C accession number AJ251502. (Somma et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	cytokinesis defective (Somma et al., 2002)
Phenotype Manifest In
	actomyosin contractile ring (Somma et al., 2002) mitotic telophase (Somma et al., 2002) mitotic telophase & spindle (Somma et al., 2002)
Detailed Description
	Statement Reference The FACS profile of S2 cells treated with RacGAP50C^{dsRNA.1110-1961} dsRNA for 72 hours differs from the profile of control cells; there is a clear increase in the 8C peak at the expense of the 2C and 4C peaks, suggesting that a substantial fraction of the cells have become polyploid. A high frequency of binucleated cells is seen. Together, these analyses indicate a frequent failure of cytokinesis in the dsRNA treated S2 cells. S2 cell cultures treated with RacGAP50C^{dsRNA.1110-1961} dsRNA show a slight increase in the number of apoptotic cells compared to control cultures. Metaphase and anaphase figures are morphologically normal in cultures of S2 cells treated with RacGAP50C^{dsRNA.1110-1961} dsRNA for 72 hours and the frequencies of anaphases relative to metaphases are comparable with those of untreated controls. However, the telophases are severely affected; many telophases have a shorter pole-to-pole length than normal (morphologically normal telophases occur at very low frequencies). These "short" telophases have typical telophase nuclei with fully decondensed chromosomes, but they lack the central spindle. A uniform F actin distribution is seen in 20-30% of the short telophases, whereas in the remaining short telophases F actin concentrates in a wide equatorial band. (Somma et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	dsRNA has been synthesised from the template and used to treat S2 cells to study the phenotypic consequences of dsRNA interference (RNAi) of the RacGAP50C gene on cytokinesis in the cells. (Somma et al., 2002)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	RacGAP50C^{dsRNA.1110-1961} tum^{dsRNA.1110-1961}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Somma et al., 2002, Mol. Biol. Cell 13(7): 2448--2460 Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells. [FBrf0151414]

Allele Dmel\tumdsRNA.1110-1961

Allele Dmel\tum^{dsRNA.1110-1961}