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General Information
Symbol
Dmel\Eip75BΔ51
Species
D. melanogaster
Name
FlyBase ID
FBal0147922
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
E75Δ51
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Imprecise excision of P{PZ}Eip75B03338 has caused a deletion removing approximately 30 kb of DNA, taking out the 4th and 5th exons of Eip75B (exons shared by all 3 isoforms (A, B & C).
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Eip75BΔ51/Eip75BΔ51 mutant mushroom body neuron clones fail to fully innervate the adult gamma lobe during regrowth at metamorphosis, and also display a weak pruning defect, but there is no defect in initial extension of these neurons, as compared to wild type.
Almost all Eip75BA81/Eip75BΔ51 mutant second instar larvae fail to molt and develop to later stages. Feeding ecdysone to these second instar larvae rescues 50% of them to the pupal stage.
Eip75BΔ51 homozygotes remain as first instar larvae for over a week before dying without any detectable morphological abnormalities. Approximately 33% of Eip75B1/Eip75BΔ51 animals die as pharate adults, with normal adult pigmentation and fully developed appendages. The animals eclose and are severely uncoordinated, displaying difficulty in walking and an inability to fly. These animals die within a week following eclosion, but appear morphologically normal with the exception of black spots that cover about one quarter of the surface of the eye. Eip75BΔ1/Eip75BΔ51 animals are viable and fertile, with no detectable phenotypes. Subsets of first and second instar Eip75BA81/Eip75BΔ51 larvae never molt to the next instar, surviving for up to a week before dying. Those that molt do so up to 12 hr late, while some die at the molt. These animals often have malformed mouthhooks or two sets of mouthhooks. Among the remainder, significant lethality occurs in the 3rd instar, and pre-pupal stages, with the rest dying as pharate adults that, while failing to eclose, have no obvious morphological defects. Approximately 20% of Eip75BA81/Eip75BΔ51 second instar larvae fail to undergo the 2nd to 3rd instar molt but nevertheless pupariate, despite maintaining 2nd instar morphology as judged by mouthhooks and anterior spiracles. This pupariation takes place approximately 88 hours after the 1st to 2nd instar molt. About 20% of the L2 prepupae (n = 85) develop to the pupal stage as evidenced by head eversion and leg and wing extension. Eip75BA81/Eip75BΔ51 second instar larvae have a reduced ecdysteroid titer, and their molting defects can be rescued by treatment with ecdysteroids.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Expression of S6kSTDE.Scer\UAS or RhebScer\UAS.cUa under the control of Scer\GAL4Tab2-201Y partially rescues the gamma lobe regrowth defects of Eip75BΔ51/Eip75BΔ51 mushroom body neuron clones. Eip75BΔ51/Eip75BΔ51, Hr51LL04325/Hr51LL04325 double mutant mushroom body neuron clones do not show a significantly more severe regrowth defect than either single mutant. Expression of Hr51Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Tab2-201Y fails to suppress the gamma lobe regrowth defects of Eip75BΔ51/Eip75BΔ51 mushroom body neuron clones.
The levels of follicle death (scored as percentage of ovarioles containing at least one dying follicle) in Eip78CΔ31;Eip75BΔ51 double heterozygote females is not significantly increased compared to either Eip78CΔ31/+ or Eip75BΔ51/+ alone.
Xenogenetic Interactions
Statement
Reference
Pioglitazone can no longer rescue the Scer\GAL4D42>Hsap\TARDBPScer\UAS.T:Avic\GFP-YFP.cEa-dependent larval turning defects in a heterozygous Eip75BΔ51 genetic background. Pioglitazone can no longer rescue the Scer\GAL4D42>Hsap\TARDBPG298S.Scer\UAS.T:Avic\GFP-YFP-dependent larval turning defects in a heterozygous Eip75BΔ51 genetic background.
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (8)