Open Close
General Information
Symbol
Dmel\crokKG06053a
Species
D. melanogaster
Name
FlyBase ID
FBal0148289
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion in the second protein coding exon.

Insertion components
P{SUPor-P}crokKG06053a
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous and crokKG06053a/Df(2L)esc-P3-0 stage 16 embryos have an elongated and convoluted dorsal trunk compared to wild-type controls. The luminal chitin matrix appears wider than normal and is amorphous in the mutant dorsal trunks. Ultrastructurally, the trachea of mutant stage 16 embryos have reduced and irregular septate junctions and the tracheal cells contain large unusual vesicle-like structures.

The paracellular diffusion barrier is defective in mutant embryos; 10kDa rhodamine-conjugated dextran injected into the body cavity diffuses rapidly into the tracheal and salivary gland lumens in mutant embryos, in contrast to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of crokScer\UAS.cNa under the control of Scer\GAL469B rescues the tracheal defects seen in crokKG06053a embryos.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Comments
Comments

Excision of the insertion can revert the lethality and the mutant tracheal phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
CG17218KG06053
CG17218KG06053a
crokKG6053
Name Synonyms
Secondary FlyBase IDs
    References (4)