At mid-stage 17, mutant embryos are indistinguishable from wild type and develop tightly encased by the eggshell. However, at late stage 17, the mutant L1 larvae separates from the eggshell, the free space being filled with liquid. The tracheae are not air-filled in the mutant L1 larvae. The mutant larvae move actively in the egg case. The mutant larvae are smaller than normal and deformed. Occasionally their cuticle detaches from the body surface, but it is not ruptured. The head skeleton appears normal. The distribution of mitochondria appears normal in stage 17 mutant embryos and mitochondrial morphology is normal in mutant first instar larvae.

Mutant embryos appear to have normal tracheal impermeability at late stage 16, but at late stage 17, defects in tracheal barrier function are seen (dextran leaks into the tracheal lumen after injection into the hemolymph).

The organisation of the septate junctions along the lateral plasma membrane appears normal in mutant first instar larvae.

Cuticle ultrastructure appears normal in mid-stage 17 mutant larvae. However, the cuticle of mutant first instar larvae is severely disorganised. Electron-lucid gaps separate the epicuticle from the envelope. The basal side of the procuticle is dishevelled and detaches from the apical surface of the epidermis. The non-laminar zone adjacent to the apical plasma membrane is missing.

Alas^KG10015 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, Zzzz\GGGGCC^30.UAS.EGFP

Alas^KG10015 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.long, Zzzz\GGGGCC^30.UAS.EGFP