Allele Dmel\14-3-3ε^ex4

General Information
Symbol	Dmel\14-3-3ε^ex4	Species	D. melanogaster
Name		FlyBase ID	FBal0148514
Feature type	allele	Associated gene	Dmel\14-3-3ε
Also Known As	D14-3-3ε^ex4

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity (Chen et al., 2003, Acevedo et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	14-3-3ε^j2B10 (Acevedo et al., 2007, Chen et al., 2003)
Nature of the lesion	Statement Reference Small deletion removing the first exon and part of the first intron. (Acevedo et al., 2007) Imprecise mobilisation of the P{lacW}14-3-3ε^j2B10 element has created this allele. (Chen et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	body size defective (with 14-3-3ε^j2B10) (Nielsen et al., 2008) female fertile (with 14-3-3ε^ex5) (Tsigkari et al., 2012) female sterile (Tsigkari et al., 2012) female sterile (with 14-3-3ε^j2B10) (Tsigkari et al., 2012) female sterile (with Df(3R)14-3-3ε^ex24) (Tsigkari et al., 2012) lethal \| partially (with Df(3R)14-3-3ε^ex24) (Acevedo et al., 2007) male fertile (with 14-3-3ε^ex5) (Tsigkari et al., 2012) male sterile (Tsigkari et al., 2012) male sterile (with 14-3-3ε^j2B10) (Tsigkari et al., 2012) male sterile (with Df(3R)14-3-3ε^ex24) (Tsigkari et al., 2012) partially lethal - majority die (with 14-3-3ε^j2B10) (Acevedo et al., 2007) partially lethal - majority die \| embryonic stage \| recessive (Acevedo et al., 2007) viable (with 14-3-3ε^ex5) (Acevedo et al., 2007) visible (with 14-3-3ε^j2B10) (Acevedo et al., 2007) visible \| recessive (Acevedo et al., 2007)
Phenotype Manifest In
	anterior crossvein (Acevedo et al., 2007) anterior crossvein (with 14-3-3ε^j2B10) (Acevedo et al., 2007) pole cell (Tsigkari et al., 2012) posterior crossvein (Acevedo et al., 2007) posterior crossvein (with 14-3-3ε^j2B10) (Acevedo et al., 2007)
Detailed Description
	Statement Reference Homozygous females lay very few eggs and heterozygous females lay significantly fewer eggs than wild type. Pole cells appears dispersed all over the embryo in heterozygous and homozygous 16-18 hours embryos with the number of pole cells per gonad being reduced compared to wild type in homozygous embryos at stages 8 and 11-12. The somatic cells of the gonad are present and appear similar in number to wild type in heterozygous and homozygous embryos. (Tsigkari et al., 2012) Homozygotes show 42% viability. 14-3-3ε^ex4/14-3-3ε^j2B10 animals show 71% viability. Homozygous embryos do not show gross morphological defects. Homozygous adults have smaller wings than control flies. 42.9% of homozygotes have defects in the anterior crossvein, while 80.9% have defects in the posterior crossvein. 26.7% of 14-3-3ε^ex4/14-3-3ε^j2B10 adults have defects in the anterior crossvein, while 82.4% have defects in the posterior crossvein. (Acevedo et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference 14-3-3ε^ex4 has partially lethal - majority die \| recessive phenotype, suppressible \| heat sensitive \| partially by 14-3-3ζ^LI.15.hs (Acevedo et al., 2007) 14-3-3ε^ex4 has partially lethal - majority die \| recessive phenotype, suppressible \| heat sensitive by 14-3-3ζ^LII.2.hs (Acevedo et al., 2007) 14-3-3ε^ex4 has partially lethal - majority die \| recessive phenotype, suppressible \| partially by Scer\GAL4^elav-C155/14-3-3ζ^I.Scer\UAS (Acevedo et al., 2007) 14-3-3ε^ex4 has partially lethal - majority die \| recessive phenotype, suppressible by Scer\GAL4^elav-C155/14-3-3ζ^II.Scer\UAS (Acevedo et al., 2007) 14-3-3ε^ex4 has visible phenotype, suppressible \| partially by 14-3-3ζ^LI.15.hs (Acevedo et al., 2007)
NOT suppressed by
Statement Reference 14-3-3ε^ex4 has visible phenotype, non-suppressible by 14-3-3ζ^LII.2.hs (Acevedo et al., 2007)
Phenotype Manifest In
Suppressed by
Statement Reference 14-3-3ε^ex4 has anterior crossvein phenotype, suppressible by 14-3-3ζ^LI.15.hs (Acevedo et al., 2007) 14-3-3ε^ex4 has posterior crossvein phenotype, suppressible \| partially by 14-3-3ζ^LI.15.hs (Acevedo et al., 2007)
NOT suppressed by
Statement Reference 14-3-3ε^ex4 has anterior crossvein phenotype, non-suppressible by 14-3-3ζ^LII.2.hs (Acevedo et al., 2007) 14-3-3ε^ex4 has pole cell phenotype, non-suppressible by 14-3-3ζ^I.Scer\UAS/Scer\GAL4^αTub84B.PL (Tsigkari et al., 2012) 14-3-3ε^ex4 has pole cell phenotype, non-suppressible by 14-3-3ζ^II.Scer\UAS/Scer\GAL4^αTub84B.PL (Tsigkari et al., 2012) 14-3-3ε^ex4 has pole cell phenotype, non-suppressible by Scer\GAL4^Act5C.PI/14-3-3ζ^I.Scer\UAS (Tsigkari et al., 2012) 14-3-3ε^ex4 has pole cell phenotype, non-suppressible by Scer\GAL4^Act5C.PI/14-3-3ζ^II.Scer\UAS (Tsigkari et al., 2012) 14-3-3ε^ex4 has pole cell phenotype, non-suppressible by Scer\GAL4^{nos.UTR.T:Hsim\VP16}/14-3-3ζ^I.Scer\UAS (Tsigkari et al., 2012) 14-3-3ε^ex4 has pole cell phenotype, non-suppressible by Scer\GAL4^{nos.UTR.T:Hsim\VP16}/14-3-3ζ^II.Scer\UAS (Tsigkari et al., 2012) 14-3-3ε^ex4 has posterior crossvein phenotype, non-suppressible by 14-3-3ζ^LII.2.hs (Acevedo et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference Expression of 14-3-3ζ[I.Scer\UAS] under the control of either Scer\GAL4[αTub84B.PL], Scer\GAL4[Act5C.PI] or Scer\GAL4[nos.UTR.T:Hsim\VP16] does not rescue the reduction in the number of pole cells per gonad seen in 14-3-3ε[ex4] embryos. Expression of 14-3-3ζ[II.Scer\UAS] under the control of either Scer\GAL4[αTub84B.PL], Scer\GAL4[Act5C.PI] or Scer\GAL4[nos.UTR.T:Hsim\VP16] does not rescue the reduction in the number of pole cells per gonad seen in 14-3-3ε[ex4] embryos. (Tsigkari et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	14-3-3ε^ex4 is rescued by 14-3-3ε^hs.PA (Acevedo et al., 2007, Acevedo et al., 2007) 14-3-3ε^ex4 is rescued by 14-3-3ε^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^tub.PU (Acevedo et al., 2007, Acevedo et al., 2007) 14-3-3ε^ex4 is rescued by Scer\GAL4^elav-C155/14-3-3ε^{Scer\UAS.T:Hsap\MYC} (Acevedo et al., 2007, Acevedo et al., 2007)
Partially rescued by	14-3-3ε^ex4 is partially rescued by 14-3-3ε^Scer\UAS.cCa/Scer\GAL4^αTub84B.PL (Tsigkari et al., 2012)
Not rescued by	14-3-3ε^ex4 is not rescued by 14-3-3ε^Scer\UAS.cCa/Scer\GAL4^Act5C.PI (Tsigkari et al., 2012)
Comments	The reduction in the number of pole cells per gonad seen in 14-3-3ε[ex4] embryos is almost completely rescued by expression of 14-3-3ε[Scer\UAS.cCa] under the control of Scer\GAL4[αTub84B.PL] but not under the control of Scer\GAL4[Act5C.PI]. (Tsigkari et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	14-3-3ε^ex4 14-3-3ε^Exc4 (Nielsen et al., 2008) 14-3-3ε^exc4 (Nielsen et al., 2008) D14-3-3ε^ex4 (Tsigkari et al., 2012, Acevedo et al., 2007, Messaritou et al., 2010) eps^ex4 (Tsigkari et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Tsigkari et al., 2012, PLoS ONE 7(5): e36702 14-3-3ε is required for germ cell migration in Drosophila. [FBrf0218503] Messaritou et al., 2010, J. Biol. Chem. 285(3): 1692--1700 Dimerization is essential for 14-3-3zeta stability and function in vivo. [FBrf0209680] Nielsen et al., 2008, Aging Cell 7(5): 688--699 14-3-3 Epsilon antagonizes FoxO to control growth, apoptosis and longevity in Drosophila. [FBrf0207166] Acevedo et al., 2007, Genetics 177(1): 239--253 In vivo functional specificity and homeostasis of Drosophila 14-3-3 proteins. [FBrf0201747] Chen et al., 2003, Cell 113(4): 457--468 Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1. [FBrf0159690]

Allele Dmel\14-3-3εex4

Allele Dmel\14-3-3ε^ex4