Allele Dmel\shot^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\shot^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0150254
Feature type	allele	Associated gene	Dmel\shot

Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Subramanian et al., 2003) in vitro construct - RNAi (Subramanian et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of shot sequences. (Subramanian et al., 2003)
Carried in construct	P{UAS-dskakRNA} (Subramanian et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| larval stage, with Scer\GAL4^how-24B, Scer\GAL4^sr-md710 (Subramanian et al., 2003)
Phenotype Manifest In
	tendon cell, with Scer\GAL4^how-24B, Scer\GAL4^sr-md710 (Subramanian et al., 2003) tendon cell & actin filament, with Scer\GAL4^how-24B, Scer\GAL4^sr-md710 (Subramanian et al., 2003) tendon cell & microtubule, with Scer\GAL4^how-24B, Scer\GAL4^sr-md710 (Subramanian et al., 2003) wing, with Scer\GAL4^Bx-MS1096 (Subramanian et al., 2003)
Detailed Description
	Statement Reference Wing blisters are seen in 100% of shot^{dsRNA.Scer\UAS}, Scer\GAL4^Bx-MS1096 animals. When shot^{dsRNA.Scer\UAS} is driven by Scer\GAL4^sr-md710 and Scer\GAL4^how-24B, larvae do not grow, remain at first and second instar stages for a few days and eventually die. Tendon cells lose their compact morphology and become very elongated (often three to four times the length of wild-type). However their association with the muscle and with the cuticle is maintained. levels of microtubules (MTs) at muscle-tendon junctions are greatly reduced. Often MTs are significantly elongated in the mutant tendon cell. Also ectopic Actin filaments are formed and detected between the cuticle and the muscle-tendon junction domains. These Actin bundles are independent of MT presence and are detected in cells in which MTs are still observed. (Subramanian et al., 2003)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	dsRNA is expressed from the template to study the phenotypic consequences of dsRNA interference (RNAi) of the shot gene. (Subramanian et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	shot^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Subramanian et al., 2003, Curr. Biol. 13(13): 1086--1095 Shortstop recruits EB1/APC1 and promotes microtubule assembly at the muscle-tendon junction. [FBrf0160988]

Allele Dmel\shotdsRNA.Scer\UAS

Allele Dmel\shot^{dsRNA.Scer\UAS}