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General Information
Symbol
Dmel\pnrEnR.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0150296
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PnrEnR, UAS-pnrENR
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS sequences drive expression of a dominant-negative form of pnr that consists the two N-terminal zinc finger domains of pnr (amino acid residues 153-293) and the en repressor domain (amino acid residues 2-298).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adults expressing pnrEnR.Scer\UAS.T:en-Rep under the control of Scer\GAL4tin.CΔ4 show an increase in heart arrest/fibrillation compared to control flies upon electrical pacing of the heart.

Animals expressing pnrEnR.Scer\UAS.T:en-Rep under the control of Scer\GAL4tin.CΔ4 in the adult stages only (a temperature sensitive Scer\GAL80 construct is used to inhibit Scer\GAL4tin.CΔ4 until this stage) show a dramatic increase in the rate of pacing-induced cardiac arrest/fibrillation, which becomes more severe with age. The susceptibility to pacing is already very high in young adult flies of this genotype.

Animals expressing pnrEnR.Scer\UAS.T:en-Rep under the control of Scer\GAL4how-24B in the adult stages only (a temperature sensitive Scer\GAL80 construct is used to inhibit Scer\GAL4how-24B until this stage) show a dramatic increase in the rate of pacing-induced cardiac arrest/fibrillation, which becomes more severe with age. Cardiac dysfunction is lower at young ages in flies of this genotype, but quickly increases with time.

Flies expressing pnrEnR.Scer\UAS.T:en-Rep under the control of Scer\GAL4tin.CΔ4 have a severely truncated lifespan.

Expression of pnrEnR.Scer\UAS.T:en-Rep in Hand-expressing cells, driven by Scer\GAL4HCH.Hand, does not ablate these cells but appears to lead to a change of cell fate.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
pnrEnR.Scer\UAS.T:Rep-en
pnrEnR.Scer\UAS.T:en-Rep
pnrEnR.Scer\UAS
pnrEnR.UAS
Name Synonyms
Secondary FlyBase IDs
    References (8)