FlyBase Allele Report: Dmel\p120ctn[308]

General Information

Symbol

Dmel\p120ctn³⁰⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0150312

Feature type

allele

Associated gene

Dmel\p120ctn

Associated Insertion(s)

Carried in Construct

Also Known As

p120³⁰⁸

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Larson et al., 2008)

loss of function allele

(Fox et al., 2005, Myster et al., 2003)

Mutagen

P-element activity

(Myster et al., 2003)

Progenitor genotype

P{SUPor-P}KG01086

(Larson et al., 2008, Myster et al., 2003)

Cytology

Description

Deletion extending from site of insertion of P{SUPor-P}KG01086 to 3' region of Stlk, deleting entire defined transcription unit of p120ctn.

(Myster et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autism spectrum disorder

CEA

(Grice et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates autism spectrum disorder

modeled by Nrx-IV⁴³⁰⁴

(Grice et al., 2015)

model of autism spectrum disorder

is exacerbated by Nrx-IV⁴³⁰⁴

(Grice et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

p120ctn³⁰⁸ mutant flies (of either sex) do not exhibit a difference in survival associated with oxidative stress from Paraquat or H[[2]]O[[2]].

There is a mild but significant difference in survival between controls and p120ctn³⁰⁸ mutant males when exposed to increase osmotic stress due to the addition of 6% NaCl to their food, but this is not mirrored in females.

p120ctn³⁰⁸ mutant males and females are significantly more sensitive to heat shock (at 37[o]C) than controls.

At 25-29[o]C, there is a significant decrease in median lifespan in p120ctn³⁰⁸ mutant males and females. These mutants also suffer a heat-sensitive decrease in maximum lifespan.

(Stefanatos et al., 2013)

p120ctn³⁰⁸ heterozygotes do not exhibit a visible phenotype nor a reduction in viability.

(Tikhmyanova et al., 2010)

p120ctn³⁰⁸ mutant eyes display supernumerary lattice cells and a partially penetrant mis-patterning of the tertiary pigment cell niche. p120ctn³⁰⁸/Df(2R)244 mutant pupae exhibit a supernumerary lattice cell phenotype similar to homozygous p120ctn³⁰⁸ retinas.

p120ctn³⁰⁸ mutant pupal eyes manifest a transient separation of inter-ommatidial pigment cells (IPCs) accompanied by an apparent breakdown of adherens junctions between IPC:IPC and IPC:primary junctions.

(Larson et al., 2008)

p120ctn³⁰⁸ mutants are viable and fertile and exhibit normal cuticles.

A fraction of p120ctn³⁰⁸ mutants exhibit slight defects in cell shape during dorsal closure. Examination of dorsal closure reveals no consistent differences between p120ctn³⁰⁸ and wild-type embryos. However, the rate of dorsal closure is slowed in p120ctn³⁰⁸ mutants. On average, wild-type embryos complete dorsal closure within 75 minutes. p120ctn³⁰⁸ mutants are significantly slower, taking 112 minutes. Despite this, p120ctn³⁰⁸ mutants complete dorsal closure without apparent defects.

(Fox et al., 2005)

Mutant third instar larvae show no significant difference in the number of dendritic ends extending from the main dorsal dendrites of the vpda neurons compared to wild type.

The density of spine-like protrusions on the v'pda neurons is significantly reduced compared to wild type in mutant larvae.

(Li et al., 2005)

Tracheal development appears normal in mutant embryos.

(Lee et al., 2003)

In many (though not all) mutant embryos the leading edge cells are irregular.

(Myster et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

p120ctn³⁰⁸ has abnormal neuroanatomy phenotype, enhanceable by Rho1^72O/Rho1[+]

(Larson et al., 2008)

p120ctn³⁰⁸ has abnormal neuroanatomy phenotype, enhanceable by α-Cat^UAS.sgGFP/Scer\GAL4^GMR.PF

(Larson et al., 2008)

p120ctn³⁰⁸ has abnormal neuroanatomy phenotype, enhanceable by shg[+]/shg^R69

(Larson et al., 2008)

Suppressed by

Statement

Reference

p120ctn³⁰⁸/Df(2R)M41A8 has abnormal cell shape | embryonic stage phenotype, suppressible by Scer\GAL4^en-e16E/Arf1^Q71L.UAS

(Greig and Bulgakova, 2020)

Enhancer of

Statement

Reference

p120ctn³⁰⁸ is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of ena⁴⁶

(Li et al., 2005)

Suppressor of

Statement

Reference

p120ctn[+]/p120ctn³⁰⁸ is a suppressor of increased cell death | heat sensitive phenotype of Scer\GAL4^sd-SG29.1, Src64B^UY1332

(Fernández et al., 2014)

p120ctn[+]/p120ctn³⁰⁸ is a suppressor of decreased cell growth rate | heat sensitive phenotype of Scer\GAL4^sd-SG29.1, Src64B^UY1332

(Fernández et al., 2014)

p120ctn[+]/p120ctn³⁰⁸ is a suppressor of increased cell death phenotype of Csk^RNAi.UAS, Scer\GAL4^ptc-559.1

(Vidal et al., 2006)

p120ctn[+]/p120ctn³⁰⁸ is a suppressor | partially of increased cell death phenotype of Csk^RNAi.UAS, Scer\GAL4^sd-SG29.1

(Vidal et al., 2006)

p120ctn³⁰⁸ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^109(2)80, ena^UAS.cUa

(Li et al., 2005)

Other

Statement

Reference

p120ctn³⁰⁸, shg[+]/shg² has partially lethal - majority die | recessive phenotype

(Myster et al., 2003)

p120ctn³⁰⁸, shg[+]/shg^R69 has partially lethal - majority die | recessive phenotype

(Myster et al., 2003)

p120ctn³⁰⁸, shg[+]/shg^g119 has partially lethal - majority die | recessive phenotype

(Myster et al., 2003)

p120ctn³⁰⁸, shg² has partially lethal - majority die phenotype

(Myster et al., 2003)

p120ctn³⁰⁸, shg^R69 has partially lethal - majority die phenotype

(Myster et al., 2003)

p120ctn³⁰⁸, shg^g119 has partially lethal - majority die phenotype

(Myster et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

p120ctn³⁰⁸ has tertiary pigment cell | increased number phenotype, enhanceable by Rho1^72O/Rho1[+]

(Larson et al., 2008)

p120ctn³⁰⁸ has interommatidial cell phenotype, enhanceable by shg[+]/shg^R69

(Larson et al., 2008)

p120ctn³⁰⁸ has phenotype, enhanceable by shg[+]/shg²

(Myster et al., 2003)

p120ctn³⁰⁸ has phenotype, enhanceable by shg[+]/shg^R69

(Myster et al., 2003)

p120ctn³⁰⁸ has phenotype, enhanceable by shg[+]/shg^g119

(Myster et al., 2003)

Suppressed by

Statement

Reference

p120ctn³⁰⁸/Df(2R)M41A8 has embryonic epidermis phenotype, suppressible by Scer\GAL4^en-e16E/Arf1^Q71L.UAS

(Greig and Bulgakova, 2020)

Enhancer of

Statement

Reference

p120ctn³⁰⁸ is an enhancer of embryonic/first instar larval cuticle phenotype of Rho1^rev220

(Fox et al., 2005)

p120ctn³⁰⁸ is an enhancer of abdominal posterior ventral multidendritic neuron vdap phenotype of ena⁴⁶

(Li et al., 2005)

p120ctn³⁰⁸/p120ctn³⁰⁸ is an enhancer of embryonic ventral epidermis phenotype of shg^R69

(Myster et al., 2003)

p120ctn³⁰⁸/p120ctn³⁰⁸ is an enhancer of embryonic ventral epidermis phenotype of shg^g119

(Myster et al., 2003)

p120ctn³⁰⁸/p120ctn³⁰⁸ is an enhancer of embryonic ventral epidermis phenotype of shg²

(Myster et al., 2003)

NOT Enhancer of

Statement

Reference

p120ctn³⁰⁸ is a non-enhancer of embryonic/first instar larval cuticle & embryonic head phenotype of Rho1^rev220

(Fox et al., 2005)

Suppressor of

Statement

Reference

p120ctn[+]/p120ctn³⁰⁸ is a suppressor of wing disc phenotype of Scer\GAL4^sd-SG29.1, Src64B^UY1332

(Fernández et al., 2014)

p120ctn[+]/p120ctn³⁰⁸ is a suppressor of zonula adherens & disc epithelium proper phenotype of Csk^RNAi.UAS, Scer\GAL4^ptc-559.1

(Vidal et al., 2006)

p120ctn³⁰⁸ is a suppressor of abdominal posterior ventral multidendritic neuron vdap phenotype of Scer\GAL4^109(2)80, ena^UAS.cUa

(Li et al., 2005)

p120ctn³⁰⁸/p120ctn³⁰⁸ is a suppressor of embryonic epidermis phenotype of arm⁴

(Myster et al., 2003)

NOT Suppressor of

Statement

Reference

p120ctn[+]/p120ctn³⁰⁸ is a non-suppressor of eye phenotype of Csk^RNAi.UAS, Scer\GAL4^GMR.PF

(Vidal et al., 2006)

p120ctn[+]/p120ctn³⁰⁸ is a non-suppressor of ommatidium phenotype of Csk^RNAi.UAS, Scer\GAL4^GMR.PF

(Vidal et al., 2006)

p120ctn³⁰⁸ is a non-suppressor of embryonic/first instar larval cuticle & embryonic head phenotype of Rho1^rev220

(Fox et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

p120ctn³⁰⁸ p130CAS¹ double heterozygotes do not exhibit a visible phenotype nor a reduction in viability.

p120ctn³⁰⁸ homozygotes in a p130CAS¹ heterozygous background exhibit severely reduced viability.

p130CAS¹ p120ctn³⁰⁸ double homozygotes exhibit severely reduced viability. These double mutants exhibit misalignment of segments and fused denticle belts, phenotypes not observed in p120ctn³⁰⁸ single mutants. Double mutants successfuly complete embryogensis and form larvae, but produce few pupae and no adult flies.

(Tikhmyanova et al., 2010)

Expression of Scer\GAL4^GMR.PF driven α-Cat^{Scer\UAS.T:Avic\GFP-sg} leads to a mild enhancement of some of the p120ctn³⁰⁸ pupal retina patterning phenotypes.

Rho1^72O dominantly enhances the recessive p120ctn³⁰⁸ supernumerary tertiary pigment cell phenotype and increases the frequency of the inter-ommatidial cell patterning errors in the pupal retina.

p120ctn³⁰⁸, shg^R69/+ mutant pupae exhibit disruption of the hexagonal inter-ommatidial precursor cell pattern with extra cells present in double layers around bristle cells in the retina.

(Larson et al., 2008)

p120ctn³⁰⁸/+ partially suppresses the loss of apical profile, delamination and subsequent migration and cell death increase seen in cells at the posterior edge of the ptc expression domain in Csk^IR.Scer\UAS Scer\GAL4^ptc-559.1 third instar larval wing discs.

(Vidal et al., 2006)

Most embryos double mutant for p120ctn³⁰⁸ and Rho1^rev220 lack head cuticle, with the remaining cuticle differing significantly in size among different embryos.

p120ctn³⁰⁸ does not substantially modify the Rho1^rev220 phenotype, nor is a novel double-mutant phenotype observed. The percentage of embryos with head holes is similar, for example 76.1% of Rho1^rev220 mutants versus 74.6% of double mutants. However double mutants do have twice as many long cuticles as Rho1^rev220 single mutants.

Rho1^rev220 p120ctn³⁰⁸ double mutants exhibit the same range of phenotypes as Rho1^rev220 single mutants during early and later stages of dorsal closure.

(Fox et al., 2005)

p120ctn³⁰⁸ enhances the decrease in the number of dendritic ends extending from the main dorsal dendrites of the vpda neurons which is seen in ena⁴⁶/+ third instar larvae.

p120ctn³⁰⁸ suppresses the increase in the number of dendritic ends of the dorsal dendrites of the vpda neurons seen in third instar larvae expressing ena^Scer\UAS.cUa under the control of Scer\GAL4^109(2)80.

(Li et al., 2005)

In shg^-/shg⁺ the viability of p120ctn³⁰⁸ homozygotes is reduced to 20-60% of their shg⁺/shg⁺ siblings.

(Myster et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

p120ctn³⁰⁸ is rescued by p120ctn^Ubi-p63E.GFP

(Stefanatos et al., 2013)

p120ctn³⁰⁸ is rescued by p120ctn^Ubi-p63E.GFP

(Larson et al., 2008)

Comments

Ubiquitous expression of a p120ctn^{Ubi-p63E.T:Avic\GFP} transgene completely rescues the p120ctn³⁰⁸ pupal retina phenotype.

(Larson et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

81638

p120ctn³⁰⁸

Notes on Origin

Discoverer

Comments

Makes no mRNA as assessed by RT-PCR.

(Myster et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

dP120ctn³⁰⁸

(Vidal et al., 2006)

p120 catenin³⁰⁸

(Fic et al., 2021)

p120³⁰⁸

(Iyer et al., 2019, Stefanatos et al., 2013, Li et al., 2005)

p120ctn³⁰⁸

(Fic et al., 2021, Greig and Bulgakova, 2020, Grice et al., 2015, Fernández et al., 2014, Tikhmyanova et al., 2010, Larson et al., 2008, Fox et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (13)

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