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General Information
Symbol
Dmel\foxoUAS.cPa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Puig
FlyBase ID
FBal0150495
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-foxo, UAS-dFoxO
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS sequences drive expression of foxo. Does not include a Tag:V5 tag (revision of data reported in FBrf0161481).
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of foxoScer\UAS.cPa under the control of Scer\GAL4ap-md544 causes the wing to bend up.
Adults expressing foxoScer\UAS.cPa under the control of Scer\GAL4Myo31DF-NP0001 show a reduced ability to clear the gut of bacteria (after infection with either Serratia marcescens or Erwinia carotovora carotovora 15) compared to controls.
Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN does not prevent the normal cell cycle exit of pupal neuroblasts.
Overexpression of foxoScer\UAS.cPa under the control of Scer\GAL80ts.αTub84B and Scer\GAL4Myo31DF-NP0001 results in reduced lipid accumulation in the anterior midgut, but it does not lead to the disruption of epithelial morphology. There is a strong decrease (nearly 50%) in stored lipid levels in the whole organism as a result of the overexpression of foxoScer\UAS.cPa under the control of Scer\GAL80ts.αTub84B and Scer\GAL4Myo31DF-NP0001. Furthermore, these flies are extremely sensitive to starvation. Overexpression of foxoScer\UAS.cPa in enterocytes leads to a small but significant decrease in food intake.
Cells in clones expressing foxoScer\UAS.cPa under the control of Scer\GAL4Act.PU in the fat body have very tiny nuclei and little to no cytoplasmic area. Expression of foxoScer\UAS.cPa under the control of Scer\GAL4GMR.PU results in a significant reduction in the number of ommatidia in the eye.
Subperineurial overexpression of foxoScer\UAS.cPa under the control of Scer\GAL4Gli-rL82 in a wild-type genetic background has only a minor effect on larval peripheral nerves.
Flies expressing foxoScer\UAS.cPa under the control of Scer\GAL4sNPF-R.2.5 show significantly increased food consumption compared to controls.
Expression of foxoScer\UAS.cPa under the control of Scer\GAL4GMR.PF results in degeneration of the eye.
Expression of foxoScer\UAS.cPa under the control of Scer\GAL4Antp-10 has no effect on lamellocyte number in unstressed conditions.
Central nervous system neuroblasts of fed larvae expressing foxoScer\UAS.cPa under the control of Scer\GAL4nab show a reduction in EdU incorporation compared to controls.
Overexpression of foxoScer\UAS.cPa under the control of Scer\GAL4GMR.PF produces a small patch of severe glazing in each eye.
Compared with controls, the age-related dysplasia of the intestinal epithelium is strongly reduced in flies expressing foxoScer\UAS.cPa under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B. Expression of foxoScer\UAS.cPa under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B results in significant lifespan shortening compared with controls. Expression of foxoScer\UAS.cPa under the control of Scer\GAL4esg-NP5130 results in significant lifespan shortening compared with controls.
Expression of foxoScer\UAS.cPa under the control of Scer\GAL4Hml.Δ does not significantly affect hemocyte proliferation in third instar larvae.
foxoScer\UAS.cPa; Scer\GAL4GMR.PF adults have small, deformed eyes. 1 day old foxoScer\UAS.cPa; Scer\GAL4Ilp2.PR adults are significantly smaller (and lighter) than wild-type.
When foxoScer\UAS.cPa is driven by Scer\GAL4ey.PH eyes become significantly smaller than wild-type. This size reduction is caused by a reduction in cell number not cell size. When foxoScer\UAS.cPa is driven by Scer\GAL4GMR.PF a severe eye phenotype is seen. many ommatidia are lost, and the remaining ones lack bristles and appear disorganised, altering the general structure of the eye. When foxoScer\UAS.cPa is driven by Scer\GAL4dpp.blk1 a significant reduction of compartment size (~20%) is seen, also caused by a reduction in cell number. When foxoScer\UAS.cPa is driven by Scer\GAL4Bx-MS1096 produces a striking reduction in wing size (~40%), also caused by a reduction in cell number.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Expression of trblScer\UAS.T:Zzzz\FLAG enhances the curved upward wing phenotype seen when foxoScer\UAS.cPa is expressed under the control of Scer\GAL4ap-md544.
Co-expression of srlEY05931 can rescue normal cell size in approximately 11% of cells in clones expressing foxoScer\UAS.cPa under the control of Scer\GAL4Act.PU in the fat body. Co-expression of srlEY05931 ameliorates the reduction in ommatidial number seen in the eyes of flies expressing foxoScer\UAS.cPa under the control of Scer\GAL4GMR.PU.
Overexpression of foxoScer\UAS.cPa in subperineurial glia under the control of Scer\GAL4Gli-rL82 suppresses the egh62d18 larval nerve phenotype.
Co-expression of foxoScer\UAS.cPa and forP1.Scer\UAS under the control of Scer\GAL4GMR.PF results in degeneration of the eye. The severity of this phenotype is enhanced by co-expression of LrrkI1915T.Scer\UAS but is not suppressed by either co-expression of Lrrk3KD.Scer\UAS or by Lrrke03680/Lrrke03680. The eye defects caused by expression of foxoScer\UAS.cPa under the control of Scer\GAL4GMR.PF are partially suppressed by fork04703/+. Co-expression of foxoScer\UAS.cPa and forP1.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 results in loss of dopaminergic neurons. This phenotype is enhanced by co-expression of LrrkI1915T.Scer\UAS. Co-expression of foxoScer\UAS.cPa and forP1.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 results in flies with reduced climbing ability. This phenotype is worsened by co-expression of LrrkI1915T.Scer\UAS. Flies co-expressing foxoScer\UAS.cPa and forP1.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 have a reduced lifespan compared to controls. Co-expression of NosScer\UAS.cMa enhances the eye defects caused by expression of foxoScer\UAS.cPa under the control of Scer\GAL4GMR.PF. Co-expression of either Gycα99BJF03176 or Gycβ100BJF03214 partially suppresses the eye defects caused by expression of foxoScer\UAS.cPa under the control of Scer\GAL4GMR.PF.
Expression of foxoScer\UAS.cPa suppresses the increase in circulating lamellocytes seen when ND75GD6309 is expressed under the control of Scer\GAL4Dot.PK.
Co-expression of cln3Scer\UAS.cTa under the control of Scer\GAL4GMR.PF enhances the foxoScer\UAS.cPa-overexpression phenotype in the eye by producing a much wider glazing.
The small, deformed eye phenotype of foxoScer\UAS.cPa; Scer\GAL4GMR.PF adults is enhanced by the presence of hepScer\UAS.cBa, but is largely suppressed by bskK53R.Scer\UAS.
Xenogenetic Interactions
Statement
Reference
Co-expression of foxoScer\UAS.cPa reverses the suppression of the grimGMR.PU eye phenotype seen when Hsap\LRRK2Scer\UAS.cCa is expressed under the control of Scer\GAL4GMR.PU.
Co-expression of Hsap\PRKG2Scer\UAS.cDa enhances the eye defects caused by expression of foxoScer\UAS.cPa under the control of Scer\GAL4GMR.PF.
Expression of foxoScer\UAS.cPa strongly suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1Scer\UAS.cSa is expressed under the control of Scer\GAL4Hml.Δ.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
foxoScer\UAS.T:SV5\V5
foxoScer\UAS.cPa
foxoUAS.cPa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Puig
Secondary FlyBase IDs
    References (29)