Allele Dmel\flw^{Scer\UAS.T:Ivir\HA1}

General Information
Symbol	Dmel\flw^{Scer\UAS.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0150498
Feature type	allele	Associated gene	Dmel\flw

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Bennett et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of flw. (Bennett et al., 2003)
Carried in construct	P{UAS-flw.HA} (Kirchner, 2007.7.19, Parker et al., 2002, Vereshchagina et al., 2004, Bennett et al., 2003, Kirchner et al., 2007, Kirchner et al., 2008, Sun et al., 2011, Jiang et al., 2011, Bennett et al., 2006)
Tagged with	T:Ivir\HA1 (Parker et al., 2002, Bennett et al., 2003, Vereshchagina et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference flw^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^arm.PS is a suppressor of lethal phenotype of NiPp1^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^arm.PS (Parker et al., 2002) flw^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Bx-MS1096 is a suppressor of visible phenotype of NiPp1^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^Bx-MS1096 (Parker et al., 2002) flw^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^Act.PU is a suppressor \| somatic clone of visible \| somatic clone phenotype of sds22^e00975 (Jiang et al., 2011) flw^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PS is a suppressor of lethal phenotype of Pp1-87B^87Bg-3/Pp1-87B¹ (Kirchner et al., 2007)
NOT Suppressor of
Statement Reference flw^{Scer\UAS.T:Ivir\HA1}/flw¹, Scer\GAL4^arm.PS is a non-suppressor of viable phenotype of Nop17l^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}, Scer\GAL4^arm.PS (Bennett et al., 2006) flw^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PS is a non-suppressor of lethal phenotype of Pp1α-96A², Pp1-87B^87Bg-3/Pp1-87B¹ (Kirchner et al., 2007)
Other
Statement Reference NiPp1^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^arm.PS, flw^{Scer\UAS.T:Ivir\HA1} has viable phenotype (Parker et al., 2002, Parker et al., 2002)
Phenotype Manifest In
Suppressor of
Statement Reference flw^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^arm.PS is a suppressor of indirect flight muscle phenotype of NiPp1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^arm.PS (Bennett et al., 2003) flw^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of NiPp1^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^Bx-MS1096 (Parker et al., 2002) flw^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^Act.PU is a suppressor \| somatic clone of eye \| somatic clone phenotype of sds22^e00975 (Jiang et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Expression of flw[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[Act.PU] significantly suppresses the eye defects seen in mosaic flies with eyes that are homozygous for sds22[e00975]. (Jiang et al., 2011) The synthetic lethality resulting from the overexpression of Nop17l[Scer\UAS.T:Zzzz\His6,T:Hsap\MYC] under the control of Scer\GAL4[arm.PS] in a hemizygous flw[1] mutant background can be rescued by the co-expression of flw[Scer\UAS.T:Ivir\HA1]. (Bennett et al., 2006) The indirect flight muscles are restored in adults expressing NiPp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS if they are also expressing flw^{Scer\UAS.T:Ivir\HA1}. (Bennett et al., 2003) The wing defects caused by expression of NiPp1^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Bx-MS1096 are almost completely suppressed by co-expression of flw^{Scer\UAS.T:Ivir\HA1}. (Parker et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	flw^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PS rescues flw⁶ (Kirchner et al., 2007) flw^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^e22c rescues flw^FP41 (Sun et al., 2011)
Comments	Expression of flw[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[e22c] rescues the oocyte polarity defects seen when the posterior follicle cells are mutant for flw[FP41]. (Sun et al., 2011)
Stocks ( 2 )
Bloomington	23702 w^; P{UAS-flw.HA}3 23703 w^; P{UAS-flw.HA}2
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	flw^{Scer\UAS.T:Ivir\HA1}
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Jiang et al., 2011, Oncogene 30(29): 3248--3260 Sds22/PP1 links epithelial integrity and tumor suppression via regulation of myosin II and JNK signaling. [FBrf0214488] Sun et al., 2011, Development 138(10): 1991--2001 Regulation of somatic myosin activity by Protein Phosphatase 1{beta} controls Drosophila oocyte polarization. [FBrf0213586] Kirchner et al., 2008, BMC Mol. Biol. 9: 36 Drosophila Uri, a PP1α binding protein, is essential for viability, maintenance of DNA integrity and normal transcriptional activity. [FBrf0204960] Kirchner et al., 2007, Genetics 176(1): 273--281 Essential, overlapping and redundant roles of the drosophila protein phosphatase 1 alpha and 1 beta genes. [FBrf0202083] Bennett et al., 2006, J. Mol. Biol. 364(2): 196--212 Towards a comprehensive analysis of the protein phosphatase 1 interactome in Drosophila. [FBrf0194330] Vereshchagina et al., 2004, Mol. Biol. Cell 15(10): 4395--4405 the essential role of PP1beta in Drosophila is to regulate nonmuscle myosin. [FBrf0180501] Bennett et al., 2003, Genetics 164(1): 235--245 Ectopic expression of inhibitors of Protein phosphatase type 1 (PP1) can be used to analyze roles of PP1 in Drosophila development. [FBrf0158995] Parker et al., 2002, Biochem. J. 368(3): 789--797 Functional interaction between nuclear inhibitor of protein phosphatase type 1 (NIPP1) and protein phosphatase type 1 (PP1) in Drosophila: consequences of over-expression of NIPP1 in flies and suppression by co-expression of PP1. [FBrf0155560]
Personal communication to FlyBase	Kirchner, 2007.7.19, Kirchner constructs and insertions. Kirchner constructs and insertions. [FBrf0200128]

Allele Dmel\flwScer\UAS.T:Ivir\HA1

Allele Dmel\flw^{Scer\UAS.T:Ivir\HA1}