Allele Dmel\baz^{Scer\UAS.P\T.T:Avic\GFP}

General Information
Symbol	Dmel\baz^{Scer\UAS.P\T.T:Avic\GFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0150555
Feature type	allele	Associated gene	Dmel\baz

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Benton and St. Johnston, 2003, Benton and St. Johnston, 2003) in vitro construct - regulatory fusion (Benton and St. Johnston, 2003, Benton and St. Johnston, 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of baz tagged with T:Avic\GFP. (Benton and St. Johnston, 2003) Construct: baz sequences are expressed under the control of Scer\UAS regulatory and P\T promoter sequences. (Benton and St. Johnston, 2003)
Carried in construct	P{UASp-baz.GFP} (Benton and St. Johnston, 2003, Benton and St. Johnston, 2003, Benton and St. Johnston, 2003, McDonald et al., 2008, Ramachandran et al., 2009, Gervais et al., 2008, Morais-de-Sá et al., 2010, Wirtz-Peitz et al., 2008, Doerflinger et al., 2010, Wirtz-Peitz et al., 2008, Becalska and Gavis, 2010, Simões et al., 2010)
Tagged with	T:Avic\GFP (Benton and St. Johnston, 2003, Benton and St. Johnston, 2003, Benton and St. Johnston, 2003)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	border follicle cell, with Scer\GAL4^c306 (McDonald et al., 2008)
Detailed Description
	Statement Reference Expression of baz[Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[mat.αTub67C.T:Hsim\VP16] has no significant effect on oocyte polarity. Maternal expression of baz[Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[mat.αTub67C.T:Hsim\VP16] has no effect on embryogenesis and the resulting larvae have normal cuticles. (Morais-de-Sá et al., 2010) Expression of baz[Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[c306] results in mild border follicle cell detachment defects as well as border cell migration defects. (McDonald et al., 2008)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference baz^{Scer\UAS.P\T.T:Avic\GFP}/Scer\GAL4¹⁴⁰⁷ is an enhancer of neuroanatomy defective phenotype of l(2)gl¹/l(2)gl⁴ (Wirtz-Peitz et al., 2008)
Suppressor of
Statement Reference baz^{Scer\UAS.P\T.T:Avic\GFP}/Scer\GAL4¹⁴⁰⁷ is a suppressor of neuroanatomy defective phenotype of aur^87Ac-3/aur³⁷ (Wirtz-Peitz et al., 2008)
Phenotype Manifest In
Enhancer of
Statement Reference baz^{Scer\UAS.P\T.T:Avic\GFP}/Scer\GAL4¹⁴⁰⁷ is an enhancer of embryonic/larval brain \| larval stage phenotype of l(2)gl¹/l(2)gl⁴ (Wirtz-Peitz et al., 2008) baz^{Scer\UAS.P\T.T:Avic\GFP}/Scer\GAL4¹⁴⁰⁷ is an enhancer of embryonic/larval neuroblast phenotype of l(2)gl¹/l(2)gl⁴ (Wirtz-Peitz et al., 2008)
Suppressor of
Statement Reference baz^{Scer\UAS.P\T.T:Avic\GFP}/Scer\GAL4¹⁴⁰⁷ is a suppressor of embryonic/larval brain \| larval stage phenotype of aur^87Ac-3/aur³⁷ (Wirtz-Peitz et al., 2008) baz^{Scer\UAS.P\T.T:Avic\GFP}/Scer\GAL4¹⁴⁰⁷ is a suppressor of embryonic/larval neuroblast phenotype of aur^87Ac-3/aur³⁷ (Wirtz-Peitz et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference The overproliferation of larval brain neuroblasts seen in l(2)gl[1]/l(2)gl[4] animals is enhanced by expression of baz[Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[1407]. The overproliferation of larval brain neuroblasts seen in aur[37]/aur[87Ac-3] animals is suppressed by expression of baz[Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[1407]. (Wirtz-Peitz et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	baz^{Scer\UAS.P\T.T:Avic\GFP}, Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI rescues baz⁴ (Morais-de-Sá et al., 2010) baz^{Scer\UAS.P\T.T:Avic\GFP}/Scer\GAL4^arm.PS rescues baz^815-8 (Benton and St. Johnston, 2003) baz^{Scer\UAS.P\T.T:Avic\GFP}/Scer\GAL4^e22c rescues baz^815-8 (Benton and St. Johnston, 2003, Benton and St. Johnston, 2003) baz^{Scer\UAS.P\T.T:Avic\GFP}/Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} rescues baz⁴ (Morais-de-Sá et al., 2010)
Comments	Expression of baz[Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[Act5C.PI] in clones in the follicular epithelium rescues the epithelial organisation defects seen in the follicle cells of baz[4] females. The epithelial organisation defects seen in baz[4] zygotic mutant embryos are rescued by zygotic expression of baz[Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[mat.αTub67C.T:Hsim\VP16]. (Morais-de-Sá et al., 2010) The addition of baz^{Scer\UAS.P\T.T:Avic\GFP} to animals with baz^815-8 follicle cell clones, efficiently rescues the epithelial polarity phenotype, the formation of the zonula adherens and cell morphology in the oocyte. (Benton and St. Johnston, 2003)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	baz^{Scer\UAS.P\T.T:Avic\GFP} baz^{Scer\UAS.T:Avic\GFP}
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Becalska and Gavis, 2010, Dev. Biol. 340(2): 528--538 Bazooka regulates microtubule organization and spatial restriction of germ plasm assembly in the Drosophila oocyte. [FBrf0210533] Doerflinger et al., 2010, Development 137(10): 1765--1773 Bazooka is required for polarisation of the Drosophila anterior-posterior axis. [FBrf0210668] Morais-de-Sá et al., 2010, Cell 141(3): 509--523 aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells. [FBrf0210663] Simões et al., 2010, Dev. Cell 19(3): 377--388 Rho-Kinase Directs Bazooka/Par-3 Planar Polarity during Drosophila Axis Elongation. [FBrf0211783] Ramachandran et al., 2009, Dev. Neurobiol. 69(9): 583--602 A critical step for postsynaptic F-actin organization: Regulation of Baz/Par-3 localization by aPKC and PTEN. [FBrf0208224] Gervais et al., 2008, Development 135(23): 3829--3838 PIP5K-dependent production of PIP2 sustains microtubule organization to establish polarized transport in the Drosophila oocyte. [FBrf0206188] McDonald et al., 2008, Curr. Biol. 18(21): 1659--1667 PAR-1 Kinase Regulates Epithelial Detachment and Directional Protrusion of Migrating Border Cells. [FBrf0206227] Wirtz-Peitz et al., 2008, Cell 135(1): 161--173 Linking cell cycle to asymmetric division: Aurora-A phosphorylates the Par complex to regulate Numb localization. [FBrf0206088] Benton and St. Johnston, 2003, Curr. Biol. 13(15): 1330--1334 A conserved oligomerization domain in Drosophila Bazooka/PAR-3 is important for apical localization and epithelial polarity. [FBrf0161479] Benton and St. Johnston, 2003, Cell 115(6): 691--704 Drosophila PAR-1 and 14-3-3 inhibit Bazooka/PAR-3 to establish complementary cortical domains in polarized cells. [FBrf0173208]
Supplementary material	Wirtz-Peitz et al., 2008, Cell 135(1): Supplemental Data. [FBrf0211096] Benton and St. Johnston, 2003, Curr. Biol. 13(15): A conserved oligomerization domain in Drosophila Bazooka/PAR-3 is important for apical localization and epithelial polarity. [FBrf0161480]

Allele Dmel\bazScer\UAS.P\T.T:Avic\GFP

Allele Dmel\baz^{Scer\UAS.P\T.T:Avic\GFP}