Allele Dmel\Tsc1^{Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\Tsc1^{Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0150577
Feature type	allele	Associated gene	Dmel\Tsc1

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Potter et al., 2002) in vitro construct - regulatory fusion (Potter et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of Tsc1 fused at the C-terminus to 6 copies of the epitope tag T:Hsap\MYC. (Potter et al., 2002)
Carried in construct	P{UAS-Tsc1.MYC} (Potter et al., 2002, Mikeladze-Dvali et al., 2005)
Tagged with	T:Hsap\MYC (Potter et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Scer\GAL4^GMR.long>Tsc1^{Scer\UAS.T:Hsap\MYC} mutants show no defects in opsin regulation. (Mikeladze-Dvali et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement Reference Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, Tsc1^{Scer\UAS.T:Hsap\MYC} is an enhancer of wing margin bristle \| somatic clone phenotype of Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} (Potter et al., 2002) Tsc1^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^ey.PH is an enhancer of eye phenotype of Scer\GAL4^ey.PH, gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} (Potter et al., 2002) Tsc1^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^ey.PH is an enhancer of ommatidium phenotype of Scer\GAL4^ey.PH, gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} (Potter et al., 2002)
Suppressor of
Statement Reference gig^{Scer\UAS.T:Zzzz\FLAG}/Tsc1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI is a suppressor \| partially of wing margin bristle \| somatic clone phenotype of Akt1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Potter et al., 2002) gig^{Scer\UAS.T:Zzzz\FLAG}/Tsc1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor \| partially of ommatidium phenotype of Akt1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Potter et al., 2002) gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}/Tsc1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI is a suppressor of wing margin bristle \| somatic clone phenotype of Akt1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Potter et al., 2002) gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}/Tsc1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Akt1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Potter et al., 2002)
NOT Suppressor of
Statement Reference Tsc1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of Akt1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Potter et al., 2002)
Other
Statement Reference Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{Scer\UAS.T:Zzzz\FLAG} has wing margin bristle \| somatic clone phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{S924A.Scer\UAS.T:Zzzz\FLAG} has eye phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{S924A.Scer\UAS.T:Zzzz\FLAG} has ommatidium phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{S924A.T1518A.Scer\UAS.T:Zzzz\FLAG} has eye phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{S924A.T1518A.Scer\UAS.T:Zzzz\FLAG} has ommatidium phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{Scer\UAS.T:Zzzz\FLAG} has eye phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{Scer\UAS.T:Zzzz\FLAG} has ommatidium phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{T437A.T1054A.Scer\UAS.T:Zzzz\FLAG} has eye phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{T437A.T1054A.Scer\UAS.T:Zzzz\FLAG} has ommatidium phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{T1518A.Scer\UAS.T:Zzzz\FLAG} has eye phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{T1518A.Scer\UAS.T:Zzzz\FLAG} has ommatidium phenotype (Potter et al., 2002, Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} has eye phenotype (Potter et al., 2002) Scer\GAL4^ey.PH, Tsc1^{Scer\UAS.T:Hsap\MYC}, gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} has ommatidium phenotype (Potter et al., 2002, Potter et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference gig^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^ey.PH; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ flies have small eyes with a 31% decrease in ommatidia numbers compared to wild-type. gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^ey.PH; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ flies have small eyes with a 75% decrease in ommatidia numbers compared to wild-type. gig^{S924A.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^ey.PH; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ flies have small eyes with an approximately 45% decrease in ommatidia numbers compared to wild-type. gig^{T1518A.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^ey.PH; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ flies have small eyes with a approximately 45% decrease in ommatidia numbers compared to wild-type. gig^{S924A.T1518A.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^ey.PH; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ flies have small eyes with a 75% decrease in ommatidia numbers compared to wild-type. gig^{T437A.T1054A.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^ey.PH; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ flies have small eyes with a 31% decrease in ommatidia numbers compared to wild-type (i.e.- results with this mutated gig are no different to those with wild-type gig.) The increase in ommatidial size seen in Akt1^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^GMR.PF flies is: unaffected by Tsc1^{Scer\UAS.T:Hsap\MYC} alone; partially suppressed by gig^{Scer\UAS.T:Zzzz\FLAG} with Tsc1^{Scer\UAS.T:Hsap\MYC}; and fully suppressed by gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} with Tsc1^{Scer\UAS.T:Hsap\MYC}. In this latter case of full suppression, ommatidial size is actually reduced slightly (by 14%) compared to wild-type. Wing margin bristles in somatic clones of gig^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^Act5C.PI; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ exhibit an average size decrease of 35%. The increase in wing margin bristle size seen in Akt1^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^Act5C.PI somatic clones is partially suppressed by gig^{Scer\UAS.T:Zzzz\FLAG} with Tsc1^{Scer\UAS.T:Hsap\MYC}, but is fully suppressed by gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} with Tsc1^{Scer\UAS.T:Hsap\MYC}. (Potter et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in the plasmid "pRmHa-3-Tsc1-Myc", transfected into S2 cells. (Potter et al., 2002)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Tsc1^{Scer\UAS.T:Hsap\MYC}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Mikeladze-Dvali et al., 2005, Cell 122(5): 775--787 The growth regulators warts/lats and melted interact in a bistable loop to specify opposite fates in Drosophila R8 photoreceptors. [FBrf0187233] Potter et al., 2002, Nat. Cell Biol. 4(9): 658--665 Akt regulates growth by directly phosphorylating Tsc2. [FBrf0152244]

Allele Dmel\Tsc1Scer\UAS.T:Hsap\MYC

Allele Dmel\Tsc1^{Scer\UAS.T:Hsap\MYC}