The disrupted formation of the presumptive posterior crossvein (assessed by p-Mad staining) in the pupal wing observed in animals expressing Gyc76CVDRC.cUa under the control of Scer\GAL4hh.PU is rescued by co-expression of TimpScer\UAS.cPa but it also leads to aberrant accumulations of the extracellular matrix.
The co-expression of TimpScer\UAS.cPa does not suppressed the long distance cell migration and scattering, and the basal delamination of cells resulting from the expression of biScer\UAS.cBa under the control of Scer\GAL4dpp.PU and Scer\GAL4C-765, respectively.
Co-expression of TimpScer\UAS.cPa suppresses the fused egg chamber phenotype seen in females expressing Mmp2Scer\UAS.cPa under the control of Scer\GAL4C587 (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to the adult stage) and restores stalk cell numbers to normal.
The cell invasion phenotype seen in cells expressing sds22GD11788 under the control of Scer\GAL4ptc-559.1 along the anterior-posterior compartment boundary of the wing disc is largely suppressed if the cells are also co-expressing TimpScer\UAS.cPa, but the abnormal apical folding phenotype is not suppressed.
Expression of TimpScer\UAS.cPa under the control of Scer\GAL4da.G32 suppresses the NMJ defects seen in Fmr1Δ50M mutant third instar larvae. The mutant over-branching and supernumerary bouton formation have been eliminated, and the accumulation of developmentally arrested satellite boutons is prevented.
Expression of Mmp2Scer\UAS.cPa in third instar larvae expressing TimpScer\UAS.cPa under the control of Scer\GAL4btl.PS (by inactivating Scer\GAL80ts.alphaTub84B through heat-shock), largely reverts the air sac primordium defects observed when TimpScer\UAS.cPa is expressed alone.
Scer\GAL4Act5C.PI>Ras85DV12.Scer\UAS, TimpScer\UAS.cPa, scrib1 eye/antennal disc clones produce a tumor mass larger than that seen in Scer\GAL4Act5C.PI>Ras85DV12.Scer\UAS, scrib1 disc clones. However, the tumor cell invasiveness observed in Scer\GAL4Act5C.PI>Ras85DV12.Scer\UAS, scrib1 clones is suppressed by expression of TimpScer\UAS.cPa.
The co-expression of TimpScer\UAS.cPa does not significantly suppress the long distance cell migration and scattering, and the basal delamination of cells resulting from the expression of Hsap\TBX2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4dpp.PU and Scer\GAL4C-765, respectively.
The increased number of neuromuscular junction boutons in TimpS1 mutant third instar larvae is somewhat decreased by Scer\GAL4how-24B-driven expression of TimpUAS.cPa, but the expression is not sufficient to rescue the slow and irregular peristaltic contractions characteristic for the mutant larvae.