FB2025_01 , released February 20, 2025
Allele: Dmel\TimpUAS.cPa
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General Information
Symbol
Dmel\TimpUAS.cPa
Species
D. melanogaster
Name
FlyBase ID
FBal0150584
Feature type
allele
Associated gene
Dmel\Timp
Associated Insertion(s)
Carried in Construct
P{UAS-Timp.P}
Also Known As
UAS-Timp
Key Links
Transgenic product class
cDNA
(Page-McCaw et al., 2003)
wild_type
(Page-McCaw et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Page-McCaw et al., 2003)
wild_type
(Page-McCaw et al., 2003)
Mutagen
in vitro construct
(Page-McCaw et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-Timp.P}
Cytology
Description

Expression of Timp cDNA (GH26186) is under the control of UASt sequences.

(Page-McCaw et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Timp
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      adult tracheal air sac | third instar larval stage | heat sensitive, with Scer\GAL4btl.PS, Scer\GAL80ts.αTub84B

      (Wang et al., 2010)

      dendritic arborizing neuron | P-stage, with Scer\GAL4ppk.PG

      (Kuo et al., 2005)

      dorsal air sac, with Scer\GAL4btl.PS

      (Guha et al., 2009)

      dorsal air sac primordium | ectopic | third instar larval stage, with Scer\GAL4ap-md544

      (Guha et al., 2009)

      dorsal air sac primordium | third instar larval stage, with Scer\GAL4btl.PS

      (Guha et al., 2009)

      dorsal air sac primordium tip cell | third instar larval stage | heat sensitive, with Scer\GAL4btl.PS, Scer\GAL80ts.αTub84B

      (Wang et al., 2010)

      egg chamber, with Scer\GAL4Act.PU

      (Deady et al., 2015)

      embryonic/larval dorsal tracheal branch | third instar larval stage, with Scer\GAL4btl.PS

      (Glasheen et al., 2009)

      embryonic/larval tracheal dorsal trunk, with Scer\GAL4btl.PS

      (Page-McCaw et al., 2003)

      embryonic/larval tracheal transverse connective | ectopic | third instar larval stage, with Scer\GAL4ap-md544

      (Guha et al., 2009)

      embryonic/larval tracheal transverse connective | third instar larval stage, with Scer\GAL4btl.PS

      (Guha et al., 2009)

      embryonic heart cardioblast, with Scer\GAL4prd.RG1

      (Raza et al., 2017)

      follicle cell, with Scer\GAL4Act.PU

      (Deady et al., 2015)

      heart primordium, with Scer\GAL4prd.RG1

      (Raza et al., 2017)

      larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4elav.PU

      (Miller et al., 2008)

      larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4repo.PU

      (Miller et al., 2008)

      larval segmental nerve branch SNa of A1-7, with Scer\GAL4elav.PU

      (Miller et al., 2008)

      larval segmental nerve branch SNa of A1-7, with Scer\GAL4repo.PU

      (Miller et al., 2008)

      larval ventral nerve cord | third instar larval stage, with Scer\GAL4repo.PU

      (Meyer et al., 2014)

      ovarian basement membrane | oogenesis stage S14, with Scer\GAL4GMR47A04

      (Deady et al., 2017)

      stalk follicle cell | increased number, with Scer\GAL4bab1-Agal4-5, Scer\GAL4ptc-559.1

      (Wang and Page-McCaw, 2014)

      tracheal section, with Scer\GAL4da.G32

      (Siller and Broadie, 2011)

      wing disc | third instar larval stage, with Scer\GAL4ap-md544

      (Guha et al., 2009)

      wing disc | third instar larval stage, with Scer\GAL4btl.PS

      (Guha et al., 2009)
      Detailed Description
      Statement
      Reference

      Stage 14 egg chambers from females expressing TimpUAS.cPa under the control of Scer\GAL4GMR47A04 show mostly an intact posterior basement membrane, instead of the mostly open posterior basement membrane observed in controls.

      (Deady et al., 2017)

      Expression of TimpScer\UAS.cPa under the control of Scer\GAL4prd.RG1 in embryos leads to heart development defects, with cardioblasts failing to extend apical protrusions and failing to form a lumen; misaligned and rounded cardioblasts are also observed, in contrast to wild type.

      (Raza et al., 2017)

      Expression of TimpScer\UAS.cPa driven by Scer\GAL4Act.PU during adulthood (using Scer\GAL80ts.αTub84B) causes a significant reduction in egg laying, there is a significant increase in mature follicle cells per female, and average time to ovulate significantly increases compared to wild type. The number of eggs laid in 6 hours after mating significantly decreases in Scer\GAL4Act.PU>TimpScer\UAS.cPa (with Scer\GAL80ts.αTub84B) females.

      Expression of TimpScer\UAS.cPa driven by Scer\GAL4GMR47A04 (along with UAS-dcr2) leads to a significant decrease in egg laying and a significant increase in ovulation time in females, compared to controls.

      (Deady et al., 2015)

      Scer\GAL4repo.PU-mediated expression of TimpScer\UAS.cPa results an elongated ventral nerve cord in third instars.

      (Meyer et al., 2014)

      Expression of TimpScer\UAS.cPa under the simultaneous control of both Scer\GAL4bab1-Agal4-5 and Scer\GAL4ptc-559.1 (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to the adult stage) results in an increase in the average number of cells in the interfollicle stalks compared to wild type.

      (Wang and Page-McCaw, 2014)

      Flies expressing TimpScer\UAS.cPa under the control of Scer\GAL4da.G32 exhibit clear tracheal deformation. ~85 of trachea exhibit at least one prominent tracheal break.

      (Siller and Broadie, 2011)

      Expression of TimpScer\UAS.cPa under the control of Scer\GAL4btl.PS (by inactivating Scer\GAL80ts.alphaTub84B through heat-shock), severely impairs air sac extension and results in a deformed structure. Instead of a single well-defined tip, air sacs display multiple tips.

      Randomly generated mutant cells expressing TimpScer\UAS.cPa under the control of Scer\GAL4btl.PS rarely populate the tip-cell territory in the air sac primordium.

      (Wang et al., 2010)

      61% of third instar larvae expressing TimpScer\UAS.cPa under the control of Scer\GAL4btl.PS display broken tracheal dorsal trunks. 63% survive to pupariation.

      (Glasheen et al., 2009)

      Expression of TimpScer\UAS.cPa under the control of Scer\GAL4btl.PS perturbs imaginal disc:tracheal association and dorsal air sac primordium growth associated with the wing disc. Extension and growth of the dorsal air sac primordia is stunted in these mutants, and the developing air sac tubes are filled with polarised epithelial cells. The adult flies that develop from these larvae lack normal dorsal air sacs.

      Expression of TimpScer\UAS.cPa under the control of Scer\GAL4ap-md544 results in ectopic basal lamina formation around the tunneled tracheae associated with the wing disc. The depth at which the disc-associated transverse connective and dorsal air sac position within the imaginal disc is altered in these animals. Tunneled trachea and dorsal air sac primordia also show ectopic extrusions from the plane of the imaginal disc. Extension and growth of the dorsal air sac primordia is stunted in these mutants, and the developing air sac tubes are filled with polarised epithelial cells.

      (Guha et al., 2009)

      Scer\GAL4elav.PU- or Scer\GAL4repo.PU-mediated expression of TimpScer\UAS.cPa results in aberrant ISNb and SNa morphology - defasciculation with loose bundling and ectopic branches.

      (Miller et al., 2008)

      Eye/antennal disc clones that express TimpScer\UAS.cPa under the control of Scer\GAL4Act5C.PI have a normal appearance.

      (Uhlirova and Bohmann, 2006)

      Overexpression of TimpScer\UAS.cPa in C4da neurons under the regulation of Scer\GAL4ppk.1.9 results in larval dendrites remaining in the extracellular space at 20 hours after pupal formation.

      (Kuo et al., 2005)

      Overexpression of TimpScer\UAS.cPa in C4da neurons under the regulation of Scer\GAL4ppk.PG results in larval dendrites remaining in the extracellular space at 20 hours after pupal formation.

      (Kuo et al., 2005)

      Expression of TimpScer\UAS.cPa in border cells, driven by Scer\GAL4slbo.2.6 does not affect the formation of the egg chamber apical cap or the migration of the border cell cluster to the oocyte.

      (Medioni and Noselli, 2005)

      Scer\GAL4btl.PS; TimpScer\UAS.cPa third instar larva have broken dorsal trunks, frequently with black melanization at the anterior tip of the broken trachea. However, 94% (n = 69) of these animals have normal posterior spiracles. 88% of Scer\GAL4btl.PS; TimpScer\UAS.cPa animals survive to third instar, 46% pupariate, 35% undergo head eversion, 33% develop head bristles, but only 7% eclose. 92% of Scer\GAL4dpp.PH; TimpScer\UAS.cPa animals survive to third instar, but only 31% pupariate, and none of these develops any further.

      (Page-McCaw et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Suppressed by
      Suppressor of
      Statement
      Reference

      TimpUAS.cPa, Scer\GAL4hh.PU is a suppressor of presumptive wing vein | pupal stage phenotype of Gyc76CVDRC.cUa, Scer\GAL4hh.PU

      (Schleede and Blair, 2015)

      Scer\GAL4Su(H).GBE/TimpUAS.cPa is a suppressor | partially of adult anterior midgut epithelium phenotype of Sox21a6

      (Zhai et al., 2015)

      TimpUAS.cPa, Scer\GAL4C587 is a suppressor of stalk follicle cell phenotype of Mmp2UAS.cPa, Scer\GAL4C587

      (Wang and Page-McCaw, 2014)

      TimpUAS.cPa, Scer\GAL4byn-Gal4 is a suppressor | partially of adult hindgut phenotype of Ras85DV12.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2012)

      TimpUAS.cPa, Scer\GAL4byn-Gal4 is a suppressor | partially of adult abdomen phenotype of Ras85DV12.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2012)

      Scer\GAL4ptc-559.1/TimpUAS.cPa is a suppressor | partially of wing disc phenotype of Scer\GAL4ptc-559.1, sds22GD11788

      (Jiang et al., 2011)

      Scer\GAL4da.G32/TimpUAS.cPa is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Fmr1Δ50M

      (Siller and Broadie, 2011)

      Scer\GAL4da.G32/TimpUAS.cPa is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Fmr1Δ50M

      (Siller and Broadie, 2011)

      TimpUAS.cPa, Scer\GAL4elav.PU is a suppressor of larval intersegmental nerve branch ISNb of A1-7 phenotype of Fas2UAS.cLa, Scer\GAL4elav.PU

      (Miller et al., 2008)

      TimpUAS.cPa, Scer\GAL4Act5C.PI is a suppressor of larval ventral nerve cord | somatic clone phenotype of Ras85DV12.UAS, Scer\GAL4Act5C.PI, scrib1

      (Uhlirova and Bohmann, 2006)

      TimpUAS.cPa, Scer\GAL4Act5C.PI is a suppressor of larval brain | somatic clone phenotype of Ras85DV12.UAS, Scer\GAL4Act5C.PI, scrib1

      (Uhlirova and Bohmann, 2006)

      TimpUAS.cPa, Scer\GAL4GMR.PF is a suppressor | partially of interommatidial bristle phenotype of Mmp2UAS.cPa, Scer\GAL4GMR.PF

      (Page-McCaw et al., 2003)

      TimpUAS.cPa, Scer\GAL4GMR.PF is a suppressor | partially of ommatidium phenotype of Mmp2UAS.cPa, Scer\GAL4GMR.PF

      (Page-McCaw et al., 2003)

      TimpUAS.cPa, Scer\GAL4dpp.PH is a suppressor of macrochaeta & thorax phenotype of Mmp1f1.UAS, Scer\GAL4dpp.PH

      (Page-McCaw et al., 2003)
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4C-765/TimpUAS.cPa is a non-suppressor of wing disc | third instar larval stage phenotype of Scer\GAL4C-765, biUAS.cPa

      (Shen et al., 2014)

      TimpUAS.cPa, Scer\GAL4dpp.PU is a non-suppressor of epithelial cell | third instar larval stage phenotype of Hsap\TBX2UAS.Tag:FLAG, Scer\GAL4dpp.PU

      (Shen et al., 2014)

      Scer\GAL4C-765/TimpUAS.cPa is a non-suppressor of epithelial cell | third instar larval stage phenotype of Scer\GAL4C-765, biUAS.cPa

      (Shen et al., 2014)

      Scer\GAL4C-765/TimpUAS.cPa is a non-suppressor of extracellular matrix | third instar larval stage phenotype of Scer\GAL4C-765, biUAS.cPa

      (Shen et al., 2014)

      Scer\GAL4dpp.PU/TimpUAS.cPa is a non-suppressor of wing disc | third instar larval stage phenotype of Scer\GAL4dpp.PU, biUAS.cPa

      (Shen et al., 2014)

      Scer\GAL4dpp.PU/TimpUAS.cPa is a non-suppressor of epithelial cell | third instar larval stage phenotype of Scer\GAL4dpp.PU, biUAS.cPa

      (Shen et al., 2014)

      TimpUAS.cPa, Scer\GAL4C-765 is a non-suppressor of wing disc | third instar larval stage phenotype of Hsap\TBX2UAS.Tag:FLAG, Scer\GAL4C-765

      (Shen et al., 2014)

      TimpUAS.cPa, Scer\GAL4C-765 is a non-suppressor of epithelial cell | third instar larval stage phenotype of Hsap\TBX2UAS.Tag:FLAG, Scer\GAL4C-765

      (Shen et al., 2014)

      TimpUAS.cPa, Scer\GAL4C-765 is a non-suppressor of extracellular matrix | third instar larval stage phenotype of Hsap\TBX2UAS.Tag:FLAG, Scer\GAL4C-765

      (Shen et al., 2014)

      TimpUAS.cPa, Scer\GAL4dpp.PU is a non-suppressor of wing disc | third instar larval stage phenotype of Hsap\TBX2UAS.Tag:FLAG, Scer\GAL4dpp.PU

      (Shen et al., 2014)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The disrupted formation of the presumptive posterior crossvein (assessed by p-Mad staining) in the pupal wing observed in animals expressing Gyc76CVDRC.cUa under the control of Scer\GAL4hh.PU is rescued by co-expression of TimpScer\UAS.cPa but it also leads to aberrant accumulations of the extracellular matrix.

      (Schleede and Blair, 2015)

      Expression of TimpScer\UAS.cPa in enteroblasts under the control of Scer\GAL4Su(H).GBE reduces tumor formation in the anterior midgut of Sox21a6 mutant flies.

      (Zhai et al., 2015)

      The co-expression of TimpScer\UAS.cPa does not suppressed the long distance cell migration and scattering, and the basal delamination of cells resulting from the expression of biScer\UAS.cBa under the control of Scer\GAL4dpp.PU and Scer\GAL4C-765, respectively.

      (Shen et al., 2014)

      Co-expression of TimpScer\UAS.cPa suppresses the fused egg chamber phenotype seen in females expressing Mmp2Scer\UAS.cPa under the control of Scer\GAL4C587 (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to the adult stage) and restores stalk cell numbers to normal.

      (Wang and Page-McCaw, 2014)

      Co-expression of TimpScer\UAS.cPa partially suppresses the dissemination of hindgut cells seen in flies expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4byn-Gal4.

      (Bangi et al., 2012)

      The cell invasion phenotype seen in cells expressing sds22GD11788 under the control of Scer\GAL4ptc-559.1 along the anterior-posterior compartment boundary of the wing disc is largely suppressed if the cells are also co-expressing TimpScer\UAS.cPa, but the abnormal apical folding phenotype is not suppressed.

      (Jiang et al., 2011)

      Expression of TimpScer\UAS.cPa under the control of Scer\GAL4da.G32 suppresses the NMJ defects seen in Fmr1Δ50M mutant third instar larvae. The mutant over-branching and supernumerary bouton formation have been eliminated, and the accumulation of developmentally arrested satellite boutons is prevented.

      Flies expressing TimpScer\UAS.cPa under the control of Scer\GAL4da.G32 in a Fmr1Δ50M mutant background are robust, healthy and fully viable.

      Homozygous Fmr1Δ50M fully suppresses the tracheal defects seen when TimpScer\UAS.cPa is expressed under the control of Scer\GAL4da.G32.

      (Siller and Broadie, 2011)

      Expression of Mmp2Scer\UAS.cPa in third instar larvae expressing TimpScer\UAS.cPa under the control of Scer\GAL4btl.PS (by inactivating Scer\GAL80ts.alphaTub84B through heat-shock), largely reverts the air sac primordium defects observed when TimpScer\UAS.cPa is expressed alone.

      (Wang et al., 2010)

      Scer\GAL4Act5C.PI>Ras85DV12.Scer\UAS, TimpScer\UAS.cPa, scrib1 eye/antennal disc clones produce a tumor mass larger than that seen in Scer\GAL4Act5C.PI>Ras85DV12.Scer\UAS, scrib1 disc clones. However, the tumor cell invasiveness observed in Scer\GAL4Act5C.PI>Ras85DV12.Scer\UAS, scrib1 clones is suppressed by expression of TimpScer\UAS.cPa.

      (Uhlirova and Bohmann, 2006)

      Loss of cell adhesion observed in induced-NijA1-161.EC1.Mtn.T:Ivir\HA1 cells is partially-suppressed through expression of TimpScer\UAS.cPa under the control of Scer\GAL4MtnA.PK.

      Co-expression of TimpScer\UAS.cPa, under the control of Scer\GAL4MtnA.PK, with NijAA.MtnA.T:Ivir\HA1 suppresses the loss of cellular adherence observed after NijAA.MtnA.T:Ivir\HA1 inducement.

      (Zhang et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference

      The co-expression of TimpScer\UAS.cPa does not significantly suppress the long distance cell migration and scattering, and the basal delamination of cells resulting from the expression of Hsap\TBX2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4dpp.PU and Scer\GAL4C-765, respectively.

      (Shen et al., 2014)
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4how-24B/TimpUAS.cPa partially rescues TimpS1

      (Shilts and Broadie, 2017)
      Comments

      The increased number of neuromuscular junction boutons in TimpS1 mutant third instar larvae is somewhat decreased by Scer\GAL4how-24B-driven expression of TimpUAS.cPa, but the expression is not sufficient to rescue the slow and irregular peristaltic contractions characteristic for the mutant larvae.

      (Shilts and Broadie, 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TimpScer\UAS.cPa
      TimpUAS.cPa
      Name Synonyms
      Secondary FlyBase IDs
        References (37)