Allele Dmel\Arpc1^Q25st

General Information
Symbol	Dmel\Arpc1^Q25st	Species	D. melanogaster
Name		FlyBase ID	FBal0150605
Feature type	allele	Associated gene	Dmel\Arpc1
Also Known As	Arpc1^Q25st, sop2^Q25st

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Hudson and Cooley, 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2L:13,829,346..13,829,346 na_change=C13829346T evidence=experimental pr_change=Q25\|Sop2-PB,Q25\|Sop2-PA reported_na_change=C?T reported_pr_change=Q25@ (Hudson and Cooley, 2002)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q25@. (Hudson and Cooley, 2002)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (with Arpc1¹) (Hudson and Cooley, 2002) lethal (with Df(2L)b84a9) (Hudson and Cooley, 2002) lethal \| recessive (Hudson and Cooley, 2002)
Phenotype Manifest In
	anterior dorsocentral bristle \| somatic clone (Hudson and Cooley, 2002) external sensory organ precursor cell IIa \| somatic clone (Rajan et al., 2009) external sensory organ precursor cell IIb \| somatic clone (Rajan et al., 2009) macrochaeta \| somatic clone (Hudson and Cooley, 2002) mechanosensory chaeta \| somatic clone (Rajan et al., 2009) myoblast \| embryonic stage (Massarwa et al., 2007) nurse cell & actin filament \| germ-line clone (Hudson and Cooley, 2002) nurse cell \| germline clone (Hudson and Cooley, 2002) nurse cell ring canal \| germline clone (Hudson and Cooley, 2002) oocyte \| germline clone (Hudson and Cooley, 2002)
Detailed Description
	Statement Reference Arpc1[Q25st] clones in the adult thorax show bristle loss. Within Arpc1[Q25st] pIIa-pIIb cells, an apical actin-rich structure (ARS) is formed as in wild type, but its apical area is markedly reduced and, in about 50% of cases, the stalk of the normal 'umbrella shape' is not formed properly. (Rajan et al., 2009) Embryos homozygous for Sop2[Q25st] commonly display unfused myoblasts. (Massarwa et al., 2007) Single cell γ neuron mutant clones in the mushroom body do not show axon growth defects. (Ng and Luo, 2004) Germ-line clones of Sop2^Q25st lead to defects in nurse cell dumping: late stage oocytes are abnormally small, while late stage nurse cells are abnormally large. By stage 10B the arrangement of actin filament bundles in the cytoplasm of mutant nurse cells is abnormally uneven, and the diameter and shape of ring canals between these cells is severely compromised. There are approximately twice as many longitudinal ridges on anterior dorso-central bristles in Sop2^Q25st mutant clones as in wild-type. A similar phenotype is seen in all other mutant macrochaete. (Hudson and Cooley, 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Statement Reference Sop2[+]/Arpc1^Q25st is a non-suppressor of mitotic cell cycle defective \| maternal effect phenotype of Abl⁴ (Grevengoed et al., 2003)
Phenotype Manifest In
NOT Suppressor of
Statement Reference Sop2[+]/Arpc1^Q25st is a non-suppressor of actin cytoskeleton \| maternal effect \| embryonic stage phenotype of Abl⁴ (Grevengoed et al., 2003) Sop2[+]/Arpc1^Q25st is a non-suppressor of spindle \| maternal effect \| embryonic stage phenotype of Abl⁴ (Grevengoed et al., 2003)
Other
Statement Reference Arpc1^Q25st, wash^Δ185 has nurse cell phenotype (Liu et al., 2009) RpII140[+]/RpII140^wimp, Arpc1^Q25st, wash^Δ185 has nurse cell phenotype (Liu et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference wash[Δ185]/+ ; Sop2[Q25st]/+ egg chambers show a loss of nurse cell integrity at stage 10a and incomplete stress fiber formation during during nurse cell dumping (stages 10b-11) compared to wild type. The actin network around the ring canals is abnormal by stage 10b. wash[Δ185]/+ ; RpII140[wimp]/+ Sop2[Q25st] egg chambers show a loss of nurse cell integrity at stage 10a and incomplete stress fiber formation during during nurse cell dumping (stages 10b-11) compared to wild type. The actin network around the ring canals is abnormal by stage 10b. (Liu et al., 2009) Heterozygosity for Sop2^Q25st does not suppress the Abl⁴ phenotype. (Grevengoed et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Arpc1^Q25st is rescued by Arpc1^+tPa (Hudson and Cooley, 2002)
Comments
Stocks ( 1 )
Bloomington	9135 P{hsFLP}12, y¹ w^*; nub¹ Arpc1^Q25st P{neoFRT}40A/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Arpc1^Q25st (Hudson and Cooley, 2002, Rajan et al., 2009, Massarwa et al., 2007, ) sop2^Q25st (Grevengoed et al., 2003) Sop2^Q25st (Liu et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Liu et al., 2009, Development 136(16): 2849--2860 Wash functions downstream of Rho and links linear and branched actin nucleation factors. [FBrf0208460] Rajan et al., 2009, Nat. Cell Biol. 11(7): 815--824 The Arp2/3 complex and WASp are required for apical trafficking of Delta into microvilli during cell fate specification of sensory organ precursors. [FBrf0208249] Massarwa et al., 2007, Dev. Cell 12(4): 557--569 WIP/WASp-based actin-polymerization machinery is essential for myoblast fusion in Drosophila. [FBrf0201865] Ng and Luo, 2004, Neuron 44(5): 779--793 Rho GTPases Regulate Axon Growth through Convergent and Divergent Signaling Pathways. [FBrf0180582] Grevengoed et al., 2003, J. Cell Biol. 163(6): 1267--1279 Balancing different types of actin polymerization at distinct sites: roles for Abelson kinase and Enabled. [FBrf0167770] Hudson and Cooley, 2002, J. Cell Biol. 156(4): 677--687 A subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complex. [FBrf0144947]

Allele Dmel\Arpc1Q25st

Allele Dmel\Arpc1^Q25st