FlyBase Allele Report: Scer\GAL4[Aug21]

General Information

Symbol

Scer\GAL4^Aug21

Species

S. cerevisiae

Name

FlyBase ID

FBal0150630

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}Aug21

Carried in Construct

Also Known As

Aug21-Gal4, Aug21, GAL4-Aug21, Aug21Gal4

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}Aug21

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval corpus allatum

(Thummel, 2010.2.24, Huang et al., 2005)

apterous-expressing neuron of the lateral cluster of the thorax Ap4

(Thummel, 2010.2.24)

second instar larval stage -- third instar larval stage

ring gland | medial

(Gaziova et al., 2004)

third instar larval stage

ring gland

(Walker et al., 2006)

wandering third instar larval stage

embryonic/larval corpus allatum

(Liu et al., 2009, Mirth et al., 2005)

embryonic/larval salivary gland

(Mirth et al., 2005)

Additional Information

Statement

Reference

Drives strong expression in the embryonic/larval corpus allatum, but not in the prothoracic gland.

(FlyBase Curators, 2017)

ScerGAL4^Aug21 drives strong expression in the embryonic/larval corpus allatum, and in Tv neurons in the thoracic ganglion.

(Thummel, 2010.2.24)

In the ring gland, ScerGAL4^Aug21 is expressed in the corpus allatum but not in the prothoracic gland.

(Mirth et al., 2005)

ScerGAL4^Aug21 drives expression in the medial ring gland, salivary gland, malpighian tubules, tracheae, and in some midgut cells.

(Gaziova et al., 2004)

Marker for

embryonic/larval corpus allatum

(Mirth et al., 2005)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell growth, with Myc^UAS.cZa

(Colombani et al., 2005)

abnormal courtship behavior, with InR^CA.UAS.cUa

(Chen et al., 2022)

abnormal developmental rate | larval stage, with grim^UAS.cNa

(Riddiford et al., 2010)

abnormal immune response | adult stage, with jhamt^GD8794

(Shianiou et al., 2023)

abnormal immune response | adult stage, with jhamt^KK103237

(Shianiou et al., 2023)

cell lethal | wandering third instar larval stage, with grim^UAS.cNa

(Liu et al., 2009)

decreased fecundity, with jhamt^GD8794

(Shianiou et al., 2023)

decreased fecundity, with jhamt^KK103237

(Shianiou et al., 2023)

decreased occurrence of cell division | prepupal stage, with grim^UAS.cNa

(Riddiford et al., 2010)

decreased occurrence of cell division | pupal stage, with grim^UAS.cNa

(Riddiford et al., 2010)

increased cell death, with grim^UAS.cNa

(Abdou et al., 2011)

lethal - all die before end of prepupal stage, with grim^UAS.cNa

(Riddiford et al., 2010, Liu et al., 2009)

long lived, with InR^K1409A.UAS

(Chen et al., 2024)

partially lethal - majority die, with Hmgcr^KK101807

(Wen et al., 2015)

short lived | female limited | heat sensitive, with InR^NIG.18402R

(Rauschenbach et al., 2013)

some die during embryonic stage, with Hmgcr^KK101807

(Wen et al., 2015)

some die during larval stage, with Hmgcr^KK101807

(Wen et al., 2015)

some die during prepupal stage | recessive, with grim^UAS.cNa

(Riddiford et al., 2010, Liu et al., 2009)

some die during P-stage, with Hmgcr^KK101807

(Wen et al., 2015)

viable, with Cyp303a1^KK105532

(Wu et al., 2019)

viable, with nvd^RNAi.1.UAS

(Yoshiyama et al., 2006)

viable, with Sumo^RNAi.UAS

(Talamillo et al., 2008)

wild-type, with jhamt^UAS.cNa

(Niwa et al., 2008)

Phenotype Manifest In

adult optic lobe | prepupal stage, with grim^UAS.cNa

(Riddiford et al., 2010)

embryonic/larval corpus allatum, with grim^UAS.cNa

(Riddiford et al., 2010, Liu et al., 2009)

embryonic/larval fat body | prepupal stage, with grim^UAS.cNa

(Liu et al., 2009)

embryonic/larval salivary gland, with grim^UAS.cNa

(Riddiford et al., 2010)

fat body, with grim^UAS.cNa

(Abdou et al., 2011)

photoreceptor cell R7 | prepupal stage, with grim^UAS.cNa

(Riddiford et al., 2010)

photoreceptor cell R8 | prepupal stage, with grim^UAS.cNa

(Riddiford et al., 2010)

ring canal, with Myc^UAS.cZa

(Colombani et al., 2005)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hmgcr^KK101807, Scer\GAL4^Aug21 has partially lethal - majority die phenotype, enhanceable by jhamt²/jhamt²

(Wen et al., 2015)

Hmgcr^KK101807, Scer\GAL4^Aug21 has some die during P-stage phenotype, enhanceable by jhamt²/jhamt²

(Wen et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^Aug21, grim^UAS.cNa has lethal | prepupal stage phenotype, suppressible by EcR^RNAi.hs.PL, Scer\GAL4^Aug21

(Liu et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^Aug21/Hmgcr^KK101807 is a suppressor | partially of abnormal courtship behavior phenotype of Ecol\lexA^VP16.Ilp2.PL, wake^RNAi.lexAop

(Chen et al., 2022)

Scer\GAL4^Aug21/InR^CA.UAS.cUa is a suppressor | partially of abnormal courtship behavior phenotype of Ecol\lexA^VP16.Ilp2.PL, wake^RNAi.lexAop

(Chen et al., 2022)

Scer\GAL4^Aug21/chico^KK107581 is a suppressor | partially of abnormal courtship behavior phenotype of Ecol\lexA^VP16.Ilp2.PL, wake^RNAi.lexAop

(Chen et al., 2022)

Scer\GAL4^Aug21/jhamt^KK103237 is a suppressor | partially of abnormal courtship behavior phenotype of Ecol\lexA^VP16.Ilp2.PL, wake^RNAi.lexAop

(Chen et al., 2022)

Scer\GAL4^Aug21/Pde4^KK108541 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Aug21/Mmus\Prkaca^act.2xUAS is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Aug21/Mmus\Prkaca^act.3xUAS is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Aug21/Mmus\Prkaca^act.4xUAS is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

jhamt^UAS.cUa/Scer\GAL4^Aug21 is a suppressor | partially of increased mortality during development phenotype of dpp^s11

(Huang et al., 2011)

EcR^RNAi.hs.PL, Scer\GAL4^Aug21 is a suppressor of lethal | prepupal stage phenotype of Scer\GAL4^Aug21, grim^UAS.cNa

(Liu et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

jhamt^UAS.cBa/Scer\GAL4^Aug21 rescues jhamt²

(Wen et al., 2015)

Fails to rescue

Scer\GAL4^Aug21/Kdm5^UASp.cSa fails to rescue Kdm5¹⁴⁰

(Drelon et al., 2019)

Scer\GAL4^Aug21/Nf1^UAS.cWa fails to rescue Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Aug21/Nf1^UAS.cWa fails to rescue Nf1^unspecified

(Walker et al., 2006)

Scer\GAL4^Aug21/Npc1a^UAS.EYFP fails to rescue Npc1a¹

(Huang et al., 2005)

Scer\GAL4^Aug21/Npc1a^UAS.EYFP fails to rescue Npc1a²

(Huang et al., 2005)

Scer\GAL4^Aug21/ecd^UAS.cGa fails to rescue ecd²

(Gaziova et al., 2004)

Scer\GAL4^Aug21/Fas2^UAS.cLa fails to rescue Fas2^spinR5

(Adam et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30137

w^*; P{GawB}Aug21/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^Aug21

Name Synonyms

Secondary FlyBase IDs

References (51)

Report Sections

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