mutants show hemocyte distribution defects: in contrast to wild-type, hemocytes are completely absent from the germband and ventral midline of stage 12 embryos, posterior hemocytes are also missing in stage 13 embryos while abnormal aggregations of hemocytes appear in the head.
mutant MARCM clones appear significantly smaller than their wild-type counterparts. The intestinal stem cell developmental programme in these mutants appears to be completely disrupted as no large polyploid epithelial enteroblasts are found within the clones. All Pvr5363
clones are comprised entirely of Dl
Infection of Pvr5363
mutant MARCM clones with P. entomophila results in the wild-type response of an increase in size and cellular architecture in intestinal stem cells, indicating a normal innate immunity response.
homozygous clones in the dorsal air sac primordium grow normally and populate the tip of the air sac primordium to the same degree as wild-type clones.
The longitudinal tracts are closer together than in the wild type in mutant embryos, giving the axon scaffold a rounded appearance in each segment. In stage 16 mutant embryos, repo
-positive glia accumulate at the midline and there are fewer repo
-positive glia in the longitudinal tract region than in wild-type embryos. No inappropriate axon crossing of the midline is detected.