Open Close
General Information
Symbol
Dmel\Pvf1UAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of McDonald
FlyBase ID
FBal0150686
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Pvf1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a full length Pvf1 cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of two copies of Pvf1Scer\UAS.cMa in the border cell cluster, driven by Scer\GAL4slbo.2.6, results in the inhibition of border cell migration in over 80% of egg chambers.

Expression of two copies of Pvf1Scer\UAS.cMa in follicle cell clones, driven by Scer\GAL4cb41, results in border cell migration defects in 93% of egg chambers.

Expression of Pvf1Scer\UAS.cMa under the control of Scer\GAL4slbo.2.6 results in border cell migration defects; in 77% of stage 10 egg chambers they have migrated 76-100% the normal distance, in 7% they have migrated 51-75% the normal distance, in 8% they have migrated 26-50% the normal distance and in 8% they have migrated 0-25% the normal distance. 87% of border cell clusters migrate normally to the oocyte in egg chambers expressing one of copy of Pvf1Scer\UAS.cMa under the control of Scer\GAL4Act5C.PI in anterior follicle cell clones. In 5% of mosaic egg chambers with this type of clone, the border cells fail to migrate away from the anterior pole and are adjacent to a clone of follicle cells misexpressing Pvf1. In 8% of mosaic egg chambers with this type of clone, the border cells localise to the side of the egg chamber less than one nurse cell away from the anterior pole and adjacent to follicle cells misexpressing Pvf1. Pvf1EP1624 has little effect on the border cell migration phenotype seen in egg chambers expressing one of copy of Pvf1Scer\UAS.cMa under the control of Scer\GAL4Act5C.PI in anterior follicle cell clones. If the egg chambers are also heterozygous or homozygous for Pvf1EP1624, in 80% of cases the border cell clusters migrate normally to the oocyte. 57% of border cell clusters migrate normally to the oocyte in egg chambers expressing one of copy of Pvf1Scer\UAS.cMa under the control of Scer\GAL4Act5C.PI in anterior follicle cell clones. In 11% of mosaic egg chambers with this type of clone, the border cells fail to migrate away from the anterior pole and are adjacent to a clone of follicle cells misexpressing Pvf1. In 19% of mosaic egg chambers with this type of clone, the border cells localise to the side of the egg chamber less than one nurse cell away from the anterior pole and adjacent to follicle cells misexpressing Pvf1. In 13% of mosaic egg chambers with this type of clone, the border cell cluster localises to the side of the egg chamber at least two nurse cells away from the anterior pole and adjacent to follicle cells misexpressing Pvf1.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The border cells migration defects caused by expression of Pvf1Scer\UAS.cMa under the control of Scer\GAL4slbo.2.6 are enhanced by tai61G1/+; 90% of double mutant stage 10 egg chambers show border cell migration defects. The border cells migration defects caused by expression of Pvf1Scer\UAS.cMa under the control of Scer\GAL4slbo.2.6 are enhanced by tai61G1/taik05809; all the double mutant egg chambers show border cell migration defects.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Pvf1Scer\UAS.cMa
Pvf1UAS.cMa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of McDonald
Secondary FlyBase IDs
    References (2)