FB2022_02, released March 29, 2022

Allele: Dmel\Pvf1UAS.cMa

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General Information
Symbol
Dmel\Pvf1UAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of McDonald
FlyBase ID
FBal0150686
Feature type
allele
Associated gene
Dmel\Pvf1
Associated Insertion(s)
Carried in Construct
P{UAS-Pvf1.M}
Also Known As
UAS-Pvf1
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(McDonald et al., 2003)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-Pvf1.M}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt regulatory sequences drive expression of a full length Pvf1 cDNA.

      (McDonald et al., 2003)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      Pvf1
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of two copies of Pvf1Scer\UAS.cMa in the border cell cluster, driven by Scer\GAL4slbo.2.6, results in the inhibition of border cell migration in over 80% of egg chambers.

      Expression of two copies of Pvf1Scer\UAS.cMa in follicle cell clones, driven by Scer\GAL4cb41, results in border cell migration defects in 93% of egg chambers.

      (McDonald et al., 2006)

      Expression of Pvf1Scer\UAS.cMa under the control of Scer\GAL4slbo.2.6 results in border cell migration defects; in 77% of stage 10 egg chambers they have migrated 76-100% the normal distance, in 7% they have migrated 51-75% the normal distance, in 8% they have migrated 26-50% the normal distance and in 8% they have migrated 0-25% the normal distance. 87% of border cell clusters migrate normally to the oocyte in egg chambers expressing one of copy of Pvf1Scer\UAS.cMa under the control of Scer\GAL4Act5C.PI in anterior follicle cell clones. In 5% of mosaic egg chambers with this type of clone, the border cells fail to migrate away from the anterior pole and are adjacent to a clone of follicle cells misexpressing Pvf1. In 8% of mosaic egg chambers with this type of clone, the border cells localise to the side of the egg chamber less than one nurse cell away from the anterior pole and adjacent to follicle cells misexpressing Pvf1. Pvf1EP1624 has little effect on the border cell migration phenotype seen in egg chambers expressing one of copy of Pvf1Scer\UAS.cMa under the control of Scer\GAL4Act5C.PI in anterior follicle cell clones. If the egg chambers are also heterozygous or homozygous for Pvf1EP1624, in 80% of cases the border cell clusters migrate normally to the oocyte. 57% of border cell clusters migrate normally to the oocyte in egg chambers expressing one of copy of Pvf1Scer\UAS.cMa under the control of Scer\GAL4Act5C.PI in anterior follicle cell clones. In 11% of mosaic egg chambers with this type of clone, the border cells fail to migrate away from the anterior pole and are adjacent to a clone of follicle cells misexpressing Pvf1. In 19% of mosaic egg chambers with this type of clone, the border cells localise to the side of the egg chamber less than one nurse cell away from the anterior pole and adjacent to follicle cells misexpressing Pvf1. In 13% of mosaic egg chambers with this type of clone, the border cell cluster localises to the side of the egg chamber at least two nurse cells away from the anterior pole and adjacent to follicle cells misexpressing Pvf1.

      (McDonald et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Statement
      Reference

      Pvf1UAS.cMa, Scer\GAL4slbo.2.6 has border follicle cell phenotype, non-enhanceable by chic[+]/chick13321

      (McDonald et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The border cells migration defects caused by expression of Pvf1Scer\UAS.cMa under the control of Scer\GAL4slbo.2.6 are enhanced by tai61G1/+; 90% of double mutant stage 10 egg chambers show border cell migration defects. The border cells migration defects caused by expression of Pvf1Scer\UAS.cMa under the control of Scer\GAL4slbo.2.6 are enhanced by tai61G1/taik05809; all the double mutant egg chambers show border cell migration defects.

      (McDonald et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Pvf1Scer\UAS.cMa
      Pvf1UAS.cMa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of McDonald
      (McDonald et al., 2003)
      Secondary FlyBase IDs
        References (2)