Expression of two copies of Pvf1Scer\UAS.cMa in the border cell cluster, driven by Scer\GAL4slbo.2.6, results in the inhibition of border cell migration in over 80% of egg chambers.
Expression of two copies of Pvf1Scer\UAS.cMa in follicle cell clones, driven by Scer\GAL4cb41, results in border cell migration defects in 93% of egg chambers.
Expression of Pvf1Scer\UAS.cMa under the control of Scer\GAL4slbo.2.6 results in border cell migration defects; in 77% of stage 10 egg chambers they have migrated 76-100% the normal distance, in 7% they have migrated 51-75% the normal distance, in 8% they have migrated 26-50% the normal distance and in 8% they have migrated 0-25% the normal distance. 87% of border cell clusters migrate normally to the oocyte in egg chambers expressing one of copy of Pvf1Scer\UAS.cMa under the control of Scer\GAL4Act5C.PI in anterior follicle cell clones. In 5% of mosaic egg chambers with this type of clone, the border cells fail to migrate away from the anterior pole and are adjacent to a clone of follicle cells misexpressing Pvf1. In 8% of mosaic egg chambers with this type of clone, the border cells localise to the side of the egg chamber less than one nurse cell away from the anterior pole and adjacent to follicle cells misexpressing Pvf1. Pvf1EP1624 has little effect on the border cell migration phenotype seen in egg chambers expressing one of copy of Pvf1Scer\UAS.cMa under the control of Scer\GAL4Act5C.PI in anterior follicle cell clones. If the egg chambers are also heterozygous or homozygous for Pvf1EP1624, in 80% of cases the border cell clusters migrate normally to the oocyte. 57% of border cell clusters migrate normally to the oocyte in egg chambers expressing one of copy of Pvf1Scer\UAS.cMa under the control of Scer\GAL4Act5C.PI in anterior follicle cell clones. In 11% of mosaic egg chambers with this type of clone, the border cells fail to migrate away from the anterior pole and are adjacent to a clone of follicle cells misexpressing Pvf1. In 19% of mosaic egg chambers with this type of clone, the border cells localise to the side of the egg chamber less than one nurse cell away from the anterior pole and adjacent to follicle cells misexpressing Pvf1. In 13% of mosaic egg chambers with this type of clone, the border cell cluster localises to the side of the egg chamber at least two nurse cells away from the anterior pole and adjacent to follicle cells misexpressing Pvf1.
Pvf1UAS.cMa, Scer\GAL4slbo.2.6 has border follicle cell phenotype, enhanceable by taik05809/tai61G1
Pvf1UAS.cMa, Scer\GAL4slbo.2.6 has border follicle cell phenotype, enhanceable by tai[+]/tai61G1
Pvf1UAS.cMa, Scer\GAL4slbo.2.6 has border follicle cell phenotype, non-enhanceable by chic[+]/chick13321
The border cells migration defects caused by expression of Pvf1Scer\UAS.cMa under the control of Scer\GAL4slbo.2.6 are enhanced by tai61G1/+; 90% of double mutant stage 10 egg chambers show border cell migration defects. The border cells migration defects caused by expression of Pvf1Scer\UAS.cMa under the control of Scer\GAL4slbo.2.6 are enhanced by tai61G1/taik05809; all the double mutant egg chambers show border cell migration defects.