|Feature type||allele||Associated gene||Dmel\Mmp2|
|Also Known As||Mmp2W307*, Mmp2W307|
|Map ( GBrowse )|
|Allele class||loss of function allele|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: W307@.
|Phenotype Manifest In|
Homozygous mutant Mmp2[W307stop] embryos exhibit defasciculation defects in 81% of hemisegments.
No Mmp1[W439stop]/Df(2R)Uba1-Mmp2 mutant third instar larvae display broken tracheal dorsal trunks. 94% survive to pupariation. Over 50% of dissected pupae show defects in head eversion.
Mmp2[W307stop] mutants show ectopic basal lamina formation around tunneled tracheae and dorsal air sac primordia of the wing disc. The depth at which the disc-associated transverse connective and dorsal air sac position within the wing disc is altered in these animals. Tunneled trachea and dorsal air sac primordium also show ectopic extrusions from the plane of the imaginal disc. Extension and growth of the dorsal air sac primordia is stunted in these mutants, and the developing air sac tubes are filled with polarised epithelial cells.
Mosaic animals in which the eyes are homozygous for Mmp2W307stop show normal location of photoreceptor nuclei in the retina.
Mmp2W307stop mutants exhibit abundant C4da neuron larval dendrites after head eversion, with approximately 8% retained after head eversion. Whereas in wild-type pupae at 20hrs after pupal formation all larval dendrites from C4da neurons are cleared from the extracellular space, in Mmp2W307stop mutants, larval dendrites that are severed from the soma remain. These larval dendrites persist to much later stages at 35 hours after pupal formation, just before the lethal phase of Mmp2W307stop at midpupariation.
83% of Mmp2W307stop mutant animals survive to 3rd instar, and 72% pupariate, but only 15% undergo head eversion, 2% develop head bristles and none eclose. 96% of Mmp2W307stop/Mmp2Df animals survive to third instar, and 89% pupariate, but only 18% undergo head eversion, 7% develop head bristles and none eclose. Mmp2W307stop mutant pupae retain their gastric caeca and proventriculus, even 22 hr after puparium formation.
|Phenotype Manifest In|
Mmp2[W307stop]/+ ; frac[Δ1]/+ double heterozygotes exhibit elevated levels of ISNb and SNa guidance defects. The incidence of ISNb mis-projection rises from 1% and 2% in frac[Δ1] and Mmp2[W307stop] single heterozygotes, respectively, to 18% in the double heterozygote.
Mmp1[Q112stop],Mmp2[W307stop] double mutant embryos show normal haemocyte migration into the tail region of the embryo.
Mmp1Q273stop Mmp2W307stop double mutant C4da clones not only exhibit dendritic branching patterns similar to wild-type clones during early pupariation, but also exhibit complete larval dendrite removal after head eversion at 20 hours after pupal formation as in wild-type controls.
The larval lethality due to Mmp1Q112stop/Mmp12 is enhanced by Mmp2218/Mmp2W307stop from 84% survival to 3rd instar and 7% pupariating to 54% survival to 3rd instar and 4% pupariating. The larval lethality of Mmp1Q112stop; Mmp2W307stop double homozygotes (73% survive to 2nd instar, 35% to 3rd instar, and none pupariate) is much greater than for either single homozygote.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 5 )|
|Secondary FlyBase IDs|
|References ( 7 )|