Allele Dmel\Mmp2^W307stop

General Information
Symbol	Dmel\Mmp2^W307stop	Species	D. melanogaster
Name		FlyBase ID	FBal0150747
Feature type	allele	Associated gene	Dmel\Mmp2
Also Known As	Mmp2^W307*, Mmp2^W307

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Page-McCaw et al., 2003)
Mutagen	ethyl methanesulfonate (Page-McCaw et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:5,501,087..5,501,087 comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=W307\|Mmp2-PB,W685\|Mmp2-PA reported_pr_change=W307@ na_change=G5501087A (Page-McCaw et al., 2003)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: W307@. (Page-McCaw et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage (Page-McCaw et al., 2003, Kuo et al., 2005) lethal \| pupal stage (with Df(2R)Uba1-Mmp2) (Glasheen et al., 2009) lethal \| pupal stage (with Mmp2^Df) (Page-McCaw et al., 2003) neuroanatomy defective \| embryonic stage (Miller et al., 2011) neuroanatomy defective \| pupal stage (Kuo et al., 2005) partially lethal - majority die \| larval stage (Page-McCaw et al., 2003)
Phenotype Manifest In
	dendritic arborizing neuron \| P-stage (Kuo et al., 2005) dorsal air sac primordium \| third instar larval stage (Guha et al., 2009) embryonic/larval proventriculus \| P-stage (Page-McCaw et al., 2003) embryonic/larval transverse connective \| third instar larval stage (Guha et al., 2009) gastric caecum \| P-stage (Page-McCaw et al., 2003) head \| pupal stage (with Df(2R)Uba1-Mmp2) (Glasheen et al., 2009) intersegmental nerve branch ISNb of A1-7 \| embryonic stage (Miller et al., 2011) wing disc \| third instar larval stage (Guha et al., 2009)
Detailed Description
	Statement Reference Homozygous mutant Mmp2[W307stop] embryos exhibit defasciculation defects in 81% of hemisegments. (Miller et al., 2011) No Mmp1[W439stop]/Df(2R)Uba1-Mmp2 mutant third instar larvae display broken tracheal dorsal trunks. 94% survive to pupariation. Over 50% of dissected pupae show defects in head eversion. (Glasheen et al., 2009) Mmp2[W307stop] mutants show ectopic basal lamina formation around tunneled tracheae and dorsal air sac primordia of the wing disc. The depth at which the disc-associated transverse connective and dorsal air sac position within the wing disc is altered in these animals. Tunneled trachea and dorsal air sac primordium also show ectopic extrusions from the plane of the imaginal disc. Extension and growth of the dorsal air sac primordia is stunted in these mutants, and the developing air sac tubes are filled with polarised epithelial cells. (Guha et al., 2009) Mosaic animals in which the eyes are homozygous for Mmp2^W307stop show normal location of photoreceptor nuclei in the retina. (Curtin et al., 2005) Mmp2^W307stop mutants exhibit abundant C4da neuron larval dendrites after head eversion, with approximately 8% retained after head eversion. Whereas in wild-type pupae at 20hrs after pupal formation all larval dendrites from C4da neurons are cleared from the extracellular space, in Mmp2^W307stop mutants, larval dendrites that are severed from the soma remain. These larval dendrites persist to much later stages at 35 hours after pupal formation, just before the lethal phase of Mmp2^W307stop at midpupariation. (Kuo et al., 2005) 83% of Mmp2^W307stop mutant animals survive to 3rd instar, and 72% pupariate, but only 15% undergo head eversion, 2% develop head bristles and none eclose. 96% of Mmp2^W307stop/Mmp2^Df animals survive to third instar, and 89% pupariate, but only 18% undergo head eversion, 7% develop head bristles and none eclose. Mmp2^W307stop mutant pupae retain their gastric caeca and proventriculus, even 22 hr after puparium formation. (Page-McCaw et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Mmp2^W307stop has neuroanatomy defective \| embryonic stage phenotype, enhanceable by frac^Δ1/frac[+] (Miller et al., 2011) Mmp2^W307stop has partially lethal - majority die \| larval stage phenotype, enhanceable by Mmp1^Q112stop/Mmp1^Q112stop (Page-McCaw et al., 2003)
Enhancer of
Statement Reference Mmp2[+]/Mmp2^W307stop is an enhancer of neuroanatomy defective \| embryonic stage phenotype of frac^Δ1 (Miller et al., 2011) Mmp2^W307stop/Mmp2²¹⁸ is an enhancer of partially lethal - majority die \| larval stage phenotype of Mmp1^Q112stop/Mmp1² (Page-McCaw et al., 2003)
Other
Statement Reference Mmp1^Q273stop, Mmp2^W307stop has neuroanatomy defective \| pupal stage phenotype (Kuo et al., 2005, Kuo et al., 2005)
Phenotype Manifest In
Enhanced by
Statement Reference Mmp2^W307stop has intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype, enhanceable by frac^Δ1/frac[+] (Miller et al., 2011)
Enhancer of
Statement Reference Mmp2[+]/Mmp2^W307stop is an enhancer of intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype of frac^Δ1 (Miller et al., 2011)
Other
Statement Reference Mmp1^Q273stop, Mmp2^W307stop has dendritic arborizing neuron \| P-stage phenotype (Kuo et al., 2005, Kuo et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference Mmp2[W307stop]/+ ; frac[Δ1]/+ double heterozygotes exhibit elevated levels of ISNb and SNa guidance defects. The incidence of ISNb mis-projection rises from 1% and 2% in frac[Δ1] and Mmp2[W307stop] single heterozygotes, respectively, to 18% in the double heterozygote. (Miller et al., 2011) Mmp1[Q112stop],Mmp2[W307stop] double mutant embryos show normal haemocyte migration into the tail region of the embryo. (Siekhaus et al., 2010) Mmp1^Q273stop Mmp2^W307stop double mutant C4da clones not only exhibit dendritic branching patterns similar to wild-type clones during early pupariation, but also exhibit complete larval dendrite removal after head eversion at 20 hours after pupal formation as in wild-type controls. (Kuo et al., 2005) The larval lethality due to Mmp1^Q112stop/Mmp1² is enhanced by Mmp2²¹⁸/Mmp2^W307stop from 84% survival to 3rd instar and 7% pupariating to 54% survival to 3rd instar and 4% pupariating. The larval lethality of Mmp1^Q112stop; Mmp2^W307stop double homozygotes (73% survive to 2nd instar, 35% to 3rd instar, and none pupariate) is much greater than for either single homozygote. (Page-McCaw et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	frac is genetically downstream of Mmp2 in axon guidance. (Miller et al., 2011)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	Mmp2^W307* (Kuo et al., 2005, Page-McCaw et al., 2003, Miller et al., 2011, Glasheen et al., 2009) Mmp2^w307* (Curtin et al., 2005) Mmp2^W307 (Guha et al., 2009) mmp2^W307 (Siekhaus et al., 2010) Mmp2^W307stop
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Miller et al., 2011, J. Neurosci. 31(14): 5335--5347 Drosophila mmp2 regulates the matrix molecule faulty attraction (frac) to promote motor axon targeting in Drosophila. [FBrf0213409] Siekhaus et al., 2010, Nat. Cell Biol. 12(6): 605--610 RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila. [FBrf0210888] Glasheen et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(8): 2659--2664 Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase. [FBrf0206699] Guha et al., 2009, Dev. Biol. 335(2): 317--326 Regulation of Drosophila matrix metalloprotease Mmp2 is essential for wing imaginal disc:trachea association and air sac tubulogenesis. [FBrf0209232] Curtin et al., 2005, J. Cell Sci. 118(12): 2649--2660 Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. [FBrf0187827] Kuo et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(42): 15230--15235 Dendrite-specific remodeling of Drosophila sensory neurons requires matrix metalloproteases, ubiquitin-proteasome, and ecdysone signaling. [FBrf0191447] Page-McCaw et al., 2003, Dev. Cell 4(1): 95--106 Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development. [FBrf0155689]

Allele Dmel\Mmp2W307stop

Allele Dmel\Mmp2^W307stop