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General Information
Symbol
Dmel\Mmp1W439stop
Species
D. melanogaster
Name
FlyBase ID
FBal0150756
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G24675316A

Amino acid change:

W439term | Mmp1-PC; W439term | Mmp1-PD; W439term | Mmp1-PE; W439term | Mmp1-PF; W439term | Mmp1-PG; W439term | Mmp1-PH; W439term | Mmp1-PI; W439term | Mmp1-PJ; W439term | Mmp1-PK; W439term | Mmp1-PL

Reported amino acid change:

W439term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change. The current annotation uses an upstream AUG 29aa upstream relative to previous reports. This accounts for the discrepency in the reported vs. annotated amino acid coordinate of the mutation.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nonsense mutation which causes a premature truncation of the Mmp1 hemopexin domain in the third of the 4 hemopexin loops.

Amino acid replacement: W439term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

7% of Mmp1W439stop mutant third instar larvae display broken tracheal dorsal trunks. 23% survive to pupariation. Dissected pupae display wing and head eversion defects.

6% of Mmp1Q273stop/Mmp1W439stop transheterozygous mutant third instar larvae display broken tracheal dorsal trunks. 78% survive to pupariation. A small number of dissected pupae show defects in head eversion.

Very few Mmp1Q273stop/Mmp1W439stop transheterozygous mutant third instar larvae expressing Mmp1Pro-pex.f1.Scer\UAS under the control of Scer\GAL4αTub84B.PL display broken tracheal dorsal trunks. The majority of dissected pupae show defects in head eversion, and some animals show additional defects in wing eversion.

Expression of Mmp1E225A.f1.Scer\UAS under the control of Scer\GAL4αTub84B.PL strongly enhances the phenotype of Mmp1Q273stop/Mmp1W439stop transheterozygous mutant third instar larvae. 15% display broken tracheal dorsal trunks and appear sluggish, and the majority of dissected pupae show defects in wing and head eversion. These phenotypes are stronger than either Mmp1Q273stop/Mmp1W439stop or Scer\GAL4αTub84B.PL driving Mmp1E225A.f1.Scer\UAS alone.

73% of Mmp1W439stop mutant animals survive to 3rd instar, 32% pupariate, but only 2% undergo head eversion, and none eclose. 90% of Mmp1W439stop/Mmp12 animals survive to third instar, but only 10% pupariate, 2% undergo head eversion, and none eclose.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Mmp1W439stop
Name Synonyms
Secondary FlyBase IDs
    References (2)