Allele Dmel\Mmp1^Q273stop

General Information
Symbol	Dmel\Mmp1^Q273stop	Species	D. melanogaster
Name		FlyBase ID	FBal0150758
Feature type	allele	Associated gene	Dmel\Mmp1
Also Known As	Mmp1^Q273*

Map ( GBrowse )
Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Page-McCaw et al., 2003)
Mutagen	ethyl methanesulfonate (Page-McCaw et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:20,563,338..20,563,338 evidence=experimental pr_change=Q302\|Mmp1-PA,Q302\|Mmp1-PB reported_pr_change=Q273@ na_change=C20563338T comment=The current annotation uses an upstream AUG 29aa upstream relative to previous reports. This accounts for the discrepency in the reported vs. annotated amino acid coordinate of the mutation. comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change. (Page-McCaw et al., 2003)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nonsense mutation leading to premature truncation of Mmp1 in the flexible hinge domain so that the predicted protein lacks the entire hemopexin domain. (Glasheen et al., 2009) Amino acid replacement: Q273@. (Page-McCaw et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	hypoactive \| larval stage (with Mmp1^W439stop), with Mmp1^{E225A.f1.Scer\UAS} (Glasheen et al., 2009) lethal \| pharate adult stage (Page-McCaw et al., 2003) lethal \| pupal stage (Kuo et al., 2005, Glasheen et al., 2009) lethal \| pupal stage (with Mmp1²) (Page-McCaw et al., 2003) lethal \| pupal stage (with Mmp1^W439stop) (Glasheen et al., 2009) lethal \| pupal stage (with Mmp1^W439stop), with Mmp1^{E225A.f1.Scer\UAS}, Scer\GAL4^αTub84B.PL (Glasheen et al., 2009) lethal \| pupal stage (with Mmp1^W439stop), with Mmp1^{Pro-pex.f1.Scer\UAS}, Scer\GAL4^αTub84B.PL (Glasheen et al., 2009) neuroanatomy defective \| pupal stage (Kuo et al., 2005) partially lethal - majority die \| larval stage (Page-McCaw et al., 2003) partially lethal - majority die \| larval stage (with Mmp1²) (Page-McCaw et al., 2003) partially lethal - majority die \| pupal stage (Page-McCaw et al., 2003) size defective \| late third instar larval stage (Glasheen et al., 2010)
Phenotype Manifest In
	dendritic arborizing neuron \| P-stage (Kuo et al., 2005) embryonic/larval dorsal branch \| third instar larval stage (Glasheen et al., 2009) embryonic/larval dorsal branch \| third instar larval stage (with Mmp1^W439stop) (Glasheen et al., 2009) embryonic/larval dorsal branch \| third instar larval stage (with Mmp1^W439stop), with Mmp1^{E225A.f1.Scer\UAS}, Scer\GAL4^αTub84B.PL (Glasheen et al., 2009) head \| pupal stage (Glasheen et al., 2009) head \| pupal stage (with Mmp1^W439stop) (Glasheen et al., 2009) head \| pupal stage (with Mmp1^W439stop), with Mmp1^{E225A.f1.Scer\UAS}, Scer\GAL4^αTub84B.PL (Glasheen et al., 2009) head \| pupal stage (with Mmp1^W439stop), with Mmp1^{Pro-pex.f1.Scer\UAS}, Scer\GAL4^αTub84B.PL (Glasheen et al., 2009) mesothoracic tergum (Page-McCaw et al., 2003) taenidium \| larval stage (Glasheen et al., 2010) wing \| pupal stage (with Mmp1^W439stop) (Glasheen et al., 2009) wing \| pupal stage (with Mmp1^W439stop), with Mmp1^{E225A.f1.Scer\UAS}, Scer\GAL4^αTub84B.PL (Glasheen et al., 2009) wing \| pupal stage (with Mmp1^W439stop), with Mmp1^{Pro-pex.f1.Scer\UAS}, Scer\GAL4^αTub84B.PL (Glasheen et al., 2009)
Detailed Description
	Statement Reference The intertaenidial distance in Mmp1[Q273stop] late third instar larvae increases slightly from the beginning of the instar, but does not elongate fully compared to wild type, indicating that the hemopexin domain of Mmp1, missing in these mutants, is not necessary for taenidial expansion. (Glasheen et al., 2010) 6% of Mmp1[Q273stop] third instar larvae display broken tracheal dorsal trunks. 59% survive to pupariation. Dissected pupae show defects in head eversion. 6% of Mmp1[Q273stop]/Mmp1[W439stop] transheterozygous mutant third instar larvae display broken tracheal dorsal trunks. 78% survive to pupariation. A small number of dissected pupae show defects in head eversion. Very few Mmp1[Q273stop]/Mmp1[W439stop] transheterozygous mutant third instar larvae expressing Mmp1[Pro-pex.f1.Scer\UAS] under the control of Scer\GAL4[αTub84B.PL] display broken tracheal dorsal trunks. The majority of dissected pupae show defects in head eversion, and some animals show additional defects in wing eversion. Expression of Mmp1[E225A.f1.Scer\UAS] under the control of Scer\GAL4[αTub84B.PL] strongly enhances the phenotype of Mmp1[Q273stop]/Mmp1[W439stop] transheterozygous mutant third instar larvae. 15% display broken tracheal dorsal trunks and appear sluggish, and the majority of dissected pupae show defects in wing and head eversion. These phenotypes are stronger than either Mmp1[Q273stop]/Mmp1[W439stop] or Scer\GAL4[αTub84B.PL] driving Mmp1[E225A.f1.Scer\UAS] alone. (Glasheen et al., 2009) Mmp1^Q273stop mutants exhibit abundant C4da neuron larval dendrites after head eversion, with approximately 3% retained after head eversion. Whereas in wild-type pupae at 20hrs after pupal formation all larval dendrites from C4da neurons are cleared from the extracellular space, in Mmp1^Q273stop mutants, larval dendrites that are severed from the soma remain. These larval dendrites persist to much later stages at 50 hours after pupal formation, just before the lethal phase of Mmp1^Q273stop at midpupariation. (Kuo et al., 2005) 94% of Mmp1^Q273stop mutant animals survive to 3rd instar, and 61% pupariate, but only 32% undergo head eversion, 26% form visible head bristles, and none eclose. Of the Mmp1^Q273stop mutant pupae that do not evert their heads, a small proportion (3/17) continue to develop adult body structures, including bristles, articulated legs, and abdominal cuticle stripes, appearing upon dissection from the pupal case as headless flies, often with cleft nota Dissection of the body cavities of these pharate adults reveals developing heads trapped inside. 87% of Mmp1^Q273stop/Mmp1² animals survive to third instar, but only 22% pupariate, 6% undergo head eversion, and none eclose. (Page-McCaw et al., 2003)
External Data
Linkouts
Interactions
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Phenotypic Class
Other
Statement Reference Mmp1^Q273stop, Mmp2^W307stop has neuroanatomy defective \| pupal stage phenotype (Kuo et al., 2005, Kuo et al., 2005)
Phenotype Manifest In
Other
Statement Reference Mmp1^Q273stop, Mmp2^W307stop has dendritic arborizing neuron \| P-stage phenotype (Kuo et al., 2005, Kuo et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference Mmp1^Q273stop Mmp2^W307stop double mutant C4da clones not only exhibit dendritic branching patterns similar to wild-type clones during early pupariation, but also exhibit complete larval dendrite removal after head eversion at 20 hours after pupal formation as in wild-type controls. (Kuo et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Mmp1^Q273* (Kuo et al., 2005, Page-McCaw et al., 2003, Glasheen et al., 2009, Glasheen et al., 2010) mmp1^Q273* (Wang et al., 2010) Mmp1^Q273stop
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Glasheen et al., 2010, Dev. Biol. 344(2): 772--783 A matrix metalloproteinase mediates airway remodeling in Drosophila. [FBrf0211435] Wang et al., 2010, Dev. Cell 18(1): 157--164 Spatial Restriction of FGF Signaling by a Matrix Metalloprotease Controls Branching Morphogenesis. [FBrf0209956] Glasheen et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(8): 2659--2664 Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase. [FBrf0206699] Kuo et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(42): 15230--15235 Dendrite-specific remodeling of Drosophila sensory neurons requires matrix metalloproteases, ubiquitin-proteasome, and ecdysone signaling. [FBrf0191447] Page-McCaw et al., 2003, Dev. Cell 4(1): 95--106 Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development. [FBrf0155689]

Allele Dmel\Mmp1Q273stop

Allele Dmel\Mmp1^Q273stop