|Feature type||allele||Associated gene||Dmel\Mmp1|
|Also Known As||Mmp1Q273*|
|Map ( GBrowse )|
|Allele class||hypomorphic allele - genetic evidence|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
comment=The current annotation uses an upstream AUG 29aa upstream relative to previous reports. This accounts for the discrepency in the reported vs. annotated amino acid coordinate of the mutation.
comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
|Associated Sequence Data|
|Nature of the lesion|
Nonsense mutation leading to premature truncation of Mmp1 in the flexible hinge domain so that the predicted protein lacks the entire hemopexin domain.
Amino acid replacement: Q273@.
|Phenotype Manifest In|
embryonic/larval dorsal branch | third instar larval stage (with Mmp1W439stop), with Mmp1E225A.f1.Scer\UAS, Scer\GAL4αTub84B.PL
The intertaenidial distance in Mmp1[Q273stop] late third instar larvae increases slightly from the beginning of the instar, but does not elongate fully compared to wild type, indicating that the hemopexin domain of Mmp1, missing in these mutants, is not necessary for taenidial expansion.
6% of Mmp1[Q273stop] third instar larvae display broken tracheal dorsal trunks. 59% survive to pupariation. Dissected pupae show defects in head eversion. 6% of Mmp1[Q273stop]/Mmp1[W439stop] transheterozygous mutant third instar larvae display broken tracheal dorsal trunks. 78% survive to pupariation. A small number of dissected pupae show defects in head eversion. Very few Mmp1[Q273stop]/Mmp1[W439stop] transheterozygous mutant third instar larvae expressing Mmp1[Pro-pex.f1.Scer\UAS] under the control of Scer\GAL4[αTub84B.PL] display broken tracheal dorsal trunks. The majority of dissected pupae show defects in head eversion, and some animals show additional defects in wing eversion. Expression of Mmp1[E225A.f1.Scer\UAS] under the control of Scer\GAL4[αTub84B.PL] strongly enhances the phenotype of Mmp1[Q273stop]/Mmp1[W439stop] transheterozygous mutant third instar larvae. 15% display broken tracheal dorsal trunks and appear sluggish, and the majority of dissected pupae show defects in wing and head eversion. These phenotypes are stronger than either Mmp1[Q273stop]/Mmp1[W439stop] or Scer\GAL4[αTub84B.PL] driving Mmp1[E225A.f1.Scer\UAS] alone.
Mmp1Q273stop mutants exhibit abundant C4da neuron larval dendrites after head eversion, with approximately 3% retained after head eversion. Whereas in wild-type pupae at 20hrs after pupal formation all larval dendrites from C4da neurons are cleared from the extracellular space, in Mmp1Q273stop mutants, larval dendrites that are severed from the soma remain. These larval dendrites persist to much later stages at 50 hours after pupal formation, just before the lethal phase of Mmp1Q273stop at midpupariation.
94% of Mmp1Q273stop mutant animals survive to 3rd instar, and 61% pupariate, but only 32% undergo head eversion, 26% form visible head bristles, and none eclose. Of the Mmp1Q273stop mutant pupae that do not evert their heads, a small proportion (3/17) continue to develop adult body structures, including bristles, articulated legs, and abdominal cuticle stripes, appearing upon dissection from the pupal case as headless flies, often with cleft nota Dissection of the body cavities of these pharate adults reveals developing heads trapped inside. 87% of Mmp1Q273stop/Mmp12 animals survive to third instar, but only 22% pupariate, 6% undergo head eversion, and none eclose.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
|Secondary FlyBase IDs|
|References ( 5 )|