Q112term | Mmp1-PC; Q112term | Mmp1-PD; Q112term | Mmp1-PE; Q112term | Mmp1-PF; Q112term | Mmp1-PG; Q112term | Mmp1-PH; Q112term | Mmp1-PI; Q112term | Mmp1-PJ; Q112term | Mmp1-PK; Q112term | Mmp1-PL
The current annotation uses an upstream AUG 29aa upstream relative to previous reports. This accounts for the discrepency in the reported vs. annotated amino acid coordinate of the mutation.
Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
Homozygous Mmp1Q112stop partially suppresses the NMJ defects seen in Fmr1Δ50M mutant third instar larvae. Synaptic bouton number is strongly rescued and increased number of synaptic branches is suppressed.
The formation of a regeneration blastema that is seen in the proximodistal portion of the leg disc after induction of wgAct5C.PS expression in the early third larval instar still occurs at the normal time if the animals are also carrying Mmp1Q112stop/+. However, the non-blastema cells (ventral leg cells) do not undergo cell-cycle arrest in the wing disc carrying Mmp1Q112stop/+, in contrast to non-blastema cells in animals in which wgAct5C.PS expression has been induced in an otherwise wild-type background.
The frequency of adults that show transdetermination (wing structures in the adult legs) after induction of wgAct5C.PS 72 hours after egg deposition is increased to 42% if the animals are also carrying Mmp1Q112stop/+.
ISNb axon guidance defects in Mmp2W307stop Mmp1Q112stop or Mmp2W307stop/Df(2R)Uba1-Mmp2 Mmp1Q112stop/Mmp12 double mutants qualitatively and quantitatively mirror the phenotypes observed in the Mmp2 single mutants.
The larval lethality due to Mmp1Q112stop/Mmp12 is enhanced by Mmp2218/Mmp2W307stop from 84% survival to 3rd instar and 7% pupariating to 54% survival to 3rd instar and 4% pupariating. The larval lethality of Mmp1Q112stop; Mmp2W307stop double homozygotes (73% survive to 2nd instar, 35% to 3rd instar, and none pupariate) is greater than for either single homozygote.