Allele Dmel\Mmp1^Q112stop

General Information
Symbol	Dmel\Mmp1^Q112stop	Species	D. melanogaster
Name		FlyBase ID	FBal0150759
Feature type	allele	Associated gene	Dmel\Mmp1
Also Known As	Mmp1^Q112*, Mmp1^Q112

Map ( GBrowse )
Allele class	loss of function allele, amorphic allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele (Glasheen et al., 2010) loss of function allele (Page-McCaw et al., 2003)
Mutagen	ethyl methanesulfonate (Page-McCaw et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:20,563,891..20,563,891 evidence=experimental pr_change=Q141\|Mmp1-PA,Q141\|Mmp1-PB reported_pr_change=Q112@ na_change=C20563891T comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change. comment=The current annotation uses an upstream AUG 29aa upstream relative to previous reports. This accounts for the discrepency in the reported vs. annotated amino acid coordinate of the mutation. (Page-McCaw et al., 2003)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q112@. (Page-McCaw et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective \| larval stage (Page-McCaw et al., 2003) lethal \| larval stage (Page-McCaw et al., 2003, Guha et al., 2009, Glasheen et al., 2009) lethal \| larval stage (with Mmp1²) (Glasheen et al., 2009) lethal \| pupal stage (with Mmp1²) (Page-McCaw et al., 2003) partially lethal - majority die \| larval stage (with Mmp1²) (Page-McCaw et al., 2003) size defective (with Mmp1²) (Glasheen et al., 2010) small body \| larval stage (with Mmp1²) (Page-McCaw et al., 2003)
Phenotype Manifest In
	embryonic/larval dorsal branch \| third instar larval stage (Glasheen et al., 2009) embryonic/larval dorsal branch \| third instar larval stage (with Mmp1²) (Glasheen et al., 2009) embryonic/larval dorsal trunk (with Mmp1²) (Page-McCaw et al., 2003) embryonic/larval trachea (with Mmp1²) (Page-McCaw et al., 2003) embryonic/larval transverse connective (with Mmp1²) (Page-McCaw et al., 2003) taenidium \| larval stage (with Mmp1²) (Glasheen et al., 2010) third instar larva (Page-McCaw et al., 2003)
Detailed Description
	Statement Reference Mmp1[2]/Mmp1[Q112stop] mutants display a similar taenidial spacing at the early third instar stage compared to controls but a reduced intertaenidial distance compared to wild type by late third instar. (Glasheen et al., 2010) 43% of Mmp1[Q112stop] mutant third instar larvae display broken tracheal dorsal trunks. None survive to pupariation. 44% of Mmp1[Q112stop]/Mmp1[2] transheterozygous mutant third instar larvae display broken tracheal dorsal trunks. (Glasheen et al., 2009) Most Mmp1[Q112stop] mutants die during early larval stages. Mutants that do develop to pupae show normal development of the imaginal discs, trachea, and dorsal air sac primordium. (Guha et al., 2009) Heterozygous leg discs which are cut and then cultured in vivo for 48 hours form a blastema, as occurs in wild-type discs, but cell division still occurs in non-blastema cells. (McClure et al., 2008) Some Mmp1^Q112stop mutants die during the second larval instar (about 30%), the rest die during the third larval instar, which lasts several days longer than in wild-type. Most Mmp1^Q112stop/Mmp1² animals survive to third instar (84%), but only 7% pupariate. All 7% undergo head eversion, but none eclose. The tracheal system phenotypes of Mmp1^Q112stop/Mmp1² larvae get more severe with age: 1. First instar: in small first instars larval trachea do not have quite as much slack as those of controls. This defect is more apparent in larger first instar larvae, where the tracheal system is visibly stretched and has no slack, especially along the transverse connectives. 20% (n = 25) of have breaks in their dorsal trunks, presumably caused by overstretching. 2. Second instar: shortened dorsal trunks; the posterior spiracles have moved inward; 44% (n = 62) have visible breaks in the dorsal trunks. 3. Third instar: The tracheal system is stretched dramatically toward the anterior of the animal, with prominent tracheal breaks in 42% (n = 52) and posterior spiracles separated from the surface of the cuticle in all. Mmp1^Q112stop/Mmp1² larvae are variable in size, but are always small than their wild-type siblings. (Page-McCaw et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Mmp1^Q112stop/Mmp1² has partially lethal - majority die \| larval stage phenotype, enhanceable by Mmp2^W307stop/Mmp2²¹⁸ (Page-McCaw et al., 2003)
Enhancer of
Statement Reference Mmp1^Q112stop/Mmp1^Q112stop is an enhancer of partially lethal - majority die \| larval stage phenotype of Mmp2^W307stop (Page-McCaw et al., 2003, Page-McCaw et al., 2003)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Mmp1[Q112stop],Mmp2[W307stop] double mutant embryos show normal haemocyte migration into the tail region of the embryo. (Siekhaus et al., 2010) The formation of a regeneration blastema that is seen in the proximodistal portion of the leg disc after induction of wg[Act5C.PS] expression in the early third larval instar still occurs at the normal time if the animals are also carrying Mmp1[Q112stop]/+. However, the non-blastema cells (ventral leg cells) do not undergo cell-cycle arrest in the wing disc carrying Mmp1[Q112stop]/+, in contrast to non-blastema cells in animals in which wg[Act5C.PS] expression has been induced in an otherwise wild-type background. The frequency of adults that show transdetermination (wing structures in the adult legs) after induction of wg[Act5C.PS] 72 hours after egg deposition is increased to 42% if the animals are also carrying Mmp1[Q112stop]/+. (McClure et al., 2008) The larval lethality due to Mmp1^Q112stop/Mmp1² is enhanced by Mmp2²¹⁸/Mmp2^W307stop from 84% survival to 3rd instar and 7% pupariating to 54% survival to 3rd instar and 4% pupariating. The larval lethality of Mmp1^Q112stop; Mmp2^W307stop double homozygotes (73% survive to 2nd instar, 35% to 3rd instar, and none pupariate) is greater than for either single homozygote. (Page-McCaw et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	Mmp1^Q112* (Page-McCaw et al., 2003, Miller et al., 2011, Glasheen et al., 2009, Glasheen et al., 2010) Mmp1^Q112 (McClure et al., 2008) mmp1^Q112 (Siekhaus et al., 2010) Mmp1^Q112stop Mmp2^Q112 (Guha et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Miller et al., 2011, J. Neurosci. 31(14): 5335--5347 Drosophila mmp2 regulates the matrix molecule faulty attraction (frac) to promote motor axon targeting in Drosophila. [FBrf0213409] Glasheen et al., 2010, Dev. Biol. 344(2): 772--783 A matrix metalloproteinase mediates airway remodeling in Drosophila. [FBrf0211435] Siekhaus et al., 2010, Nat. Cell Biol. 12(6): 605--610 RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila. [FBrf0210888] Glasheen et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(8): 2659--2664 Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase. [FBrf0206699] Guha et al., 2009, Dev. Biol. 335(2): 317--326 Regulation of Drosophila matrix metalloprotease Mmp2 is essential for wing imaginal disc:trachea association and air sac tubulogenesis. [FBrf0209232] McClure et al., 2008, Dev. Biol. 319(1): 68--77 Three genes control the timing, the site and the size of blastema formation in Drosophila. [FBrf0205073] Page-McCaw et al., 2003, Dev. Cell 4(1): 95--106 Drosophila matrix metalloproteinases are required for tissue remodeling, but not embryonic development. [FBrf0155689]

Allele Dmel\Mmp1Q112stop

Allele Dmel\Mmp1^Q112stop