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General Information
Symbol
Dmel\Doc2UAS.cRa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Reim
FlyBase ID
FBal0150885
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Doc2
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the Doc2 c6.2 cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Doc2UAS.cRa under the control of Scer\GAL4elav-C155 increases mitotic proliferation of neuroblasts and their daughter cells in the ventral nerve cord during late embryogenesis, compared to controls; expression under the control of Scer\GAL4vg.int2.1 in the larval wing disc has only a limited effect on the number of neuroblasts and their daughter cells, compared to controls.

Expression of Doc2Scer\UAS.cRa in the dorsal mesoderm in stage 11-12 embryos, driven by Scer\GAL4tin.cBa, results in the formation of additional cardioblasts, but a reduction in the number of odd-positive pericardial cells. However, eve-positive pericardial cells and dorsal muscle are less affected. The nuclei of the somatic mesoderm in dorsolateral areas are arranged irregularly. Expression of Doc2Scer\UAS.cRa throughout the mesoderm of the embryo, driven by Scer\GAL4twi.2PE, results in the formation of extra cardioblasts, an effect that is stronger in dorsal areas of the mesoderm.

Expression of Doc2Scer\UAS.cRa under the control of Scer\GAL4dpp.blk1 results in wings which lack distal structures and have broadened wing veins. Antennae lack aristae and legs are shortened.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Enhancer of
Other
Phenotype Manifest In
Enhanced by
Enhancer of
Statement
Reference
Suppressor of
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of tllUAS.cKa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA and Doc2UAS.cRa under the control of Scer\GAL4elav-C155 significantly increases total cell number and the number of neuroblasts in the late embryonic ventral nerve cord, compared to controls; expression under the control of Scer\GAL4wg.PU results in a trend towards increased symmetric (abnormal) neuroblast division and significantly increased asymmetric (normal) daughter (ganglion mother cell) division as well as a significant increase in the number of cells in the NB5-6 lineage, compared to controls; expression under the control of Scer\GAL4pros.PMG significantly increases the number of neuroblasts and daughters in S-phase, with no effect on cell cycle speed, compared to controls; expression under the control of Scer\GAL4da.PU increases the number of mitotic cells, neuroblasts, daughters and neurons, and reduces the number of glia, with a thickening of the CNS, compared to controls.

Expression of Doc2Scer\UAS.cRa, driven by Scer\GAL4tin.cBa, results in efficient rescue of cardioblast specification in a Df(3L)DocA mutant background. When Doc2Scer\UAS.cRa is coexpressed with either tinScer\UAS.cYa or pnrScer\UAS.cHa, or both transgenes together, large numbers of extra cardioblasts are formed. Expression of all three transgenes results in double the amount of cardioblasts compared to wild type. Coexpression of Doc2Scer\UAS.cRa, tinScer\UAS.cYa, and pnrScer\UAS.cHa, under the control of Scer\GAL4twi.2PE, causes widespread ectopic cardioblast formation to a much greater extent than expression of Doc2Scer\UAS.cRa alone or in combination with tinScer\UAS.cYa plus pnrScer\UAS.cHa.

Expression of Doc2Scer\UAS.cRa under the control of Scer\GAL4c381 rescues the germ band retraction defects of Df(3L)DocA embryos.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Doc2Scer\UAS.cRa
Doc2UAS.cRa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Reim
Secondary FlyBase IDs
    References (6)