|Feature type||allele||Associated gene||Dmel\Acp70A|
|Also Known As||SP0|
|Mutagen||I-CreI endonuclease, ends-in gene targeting|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Phenotype Manifest In|
Wild-type female flies mated to Acp70A/Df(3L)Δ130 mutant males exhibit a severe reduction in egg-laying per female 48 hours after mating. Approximately 80% of these females copulate again within 1 hour after initial mating and appear receptive to mating. Acp70A heterozygous males elicit a robust post-mating response from wild-type females.
Females previously mated to Acp70A/Df(3L)Δ130 males remate more frequently and sooner than do females mated to control males. Females mated to Acp70A/Df(3L)Δ130 males lay significantly fewer eggs in the period between their first and second matings than females mated to control males.
Females mated to Acp70A/Df(3L)Δ130 males do not show a reduction in receptivity to courting males and do not increase their egg-laying rate, in contrast to wild-type mated females.
Wild-type virgin females show the same receptivity towards a naive Acp70A0/Df(3L)Δ130 male as control females. However, these females lay very few eggs after mating, in contrast to control females. If the females that were mated to Acp70A0/Df(3L)Δ130 males are then tested for receptivity towards a second naive male after being allowed to lay eggs for 48 hours, they mate again at a high frequency and do not actively reject the second male, in contrast to control females tested under the same conditions.
Acp70A0 males fail to significantly induce feeding in females after mating. They exhibit courtship amd mating rates similar to those of controls and successfully fertilise females.
Females mated to Acp70A0 males exhibit only short term post-mating responses (PMR, e.g. increase in egg laying and reduction in receptivity). After two days the PMR are lacking, while in wild-type controls PMRs last for over 5 days.
Females mated with Acp70A0/Df(3L)Δ130 males show only a slight increase in oviposition on day 1 after mating and on the following days, the oviposition rate does not differ from the rate of unmated females (in contrast to females mated to wild-type males which show a considerable increase in oviposition in the first 4 days after mating). Females mated to Acp70A0/Df(3L)Δ130 males show a strong reduction of receptivity only 4 hours after copulation. 12 and 24 hours after copulation, receptivity is more than 60%. After 48 hours, receptivity has increased to virgin levels. Sperm of Acp70A0/Df(3L)Δ130 males are motile and are transferred and stored correctly.
|Phenotype Manifest In|
Wild-type mated females often excrete fecal deposits which are extremely concentrated, referred to as reproductive oblong deposits (RODs), compared to the fecal deposits of wild-type males and wild-type virgin females. ovo[D1] females which have been mated to Acp70A/Df(3L)Δ130 males do not produce RODs.
Female flies expressing rpr[Scer\UAS.C] under the control of Scer\GAL4[Ilp2.215-3] do not show a significantly reduced re-mating rate 5 hours after mating to Acp70A flies. Female Ilp2 flies show a significantly reduced re-mating rate 5 hours after mating to Acp70A flies. Female Ilp3 flies do not show a significantly reduced re-mating rate 5 hours after mating to Acp70A flies. Female Df(3L)Ilp2-3 flies show a significantly reduced re-mating rate 5 hours after mating to Acp70A flies.
|Complementation & Rescue Data|
|Not rescued by|
Expression of Acp70A[+t1893] rescues egg-laying rate and post-mating behavioral responses and receptivity to approximately wild-type levels. Expression of Acp70A[Mip] fails to rescue egg-laying rate and post-mating behavioral responses and receptivity to approximately wild-type levels in wild-type female flies that have been mated to Acp70A/Df(3L)Δ130 mutant males.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
|Secondary FlyBase IDs|
|References ( 12 )|