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General Information
Symbol
Dmel\SP0
Species
D. melanogaster
Name
FlyBase ID
FBal0150995
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A stop codon has been introduced into the signal peptide of SP.

Insertion components
TI{TI}SP0
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Female flies mated with SP0/Df(3L)Δ130 males display shorter mating refractory periods after copulation than females mated with control males.

The electrophysiological response of sex peptide abdominal ganglion (SAG) neurons following light-activation of sex peptide sensory neurons (SPSN) in SP0 females that have been mated with wild type males is indistinguishable from controls. Many wild type females that have been mated with SP0 males show an impaired response, although some still have a physiology similar to that of females mated to SP[+] males. Females that have been mated to SP0 males show increased receptivity compared to controls, but this is still significantly lower than in virgin flies. SP0-mated females that actively reject males post-mating tend to also exhibit an impaired SAG-neuron response.

Females mated with SP0 mutant males behave very much like virgins.

Females mated to Acp70A0/Df(3L)Δ130 males show high re-mating activity (comparable to those of virgin female controls) at 48 hours after the first mating.

Acp70A0/Df(3L)Δ130 males court females marginally significantly more than Acp70A[+] males.

Wild-type female flies mated to Acp70A0/Df(3L)Δ130 mutant males exhibit a severe reduction in egg-laying per female 48 hours after mating. Approximately 80% of these females copulate again within 1 hour after initial mating and appear receptive to mating.

Acp70A0 heterozygous males elicit a robust post-mating response from wild-type females.

Females previously mated to Acp70A0/Df(3L)Δ130 males remate more frequently and sooner than do females mated to control males.

Females mated to Acp70A0/Df(3L)Δ130 males lay significantly fewer eggs in the period between their first and second matings than females mated to control males.

Females mated to Acp70A0/Df(3L)Δ130 males do not show a reduction in receptivity to courting males and do not increase their egg-laying rate, in contrast to wild-type mated females.

Wild-type virgin females show the same receptivity towards a naive Acp70A0/Df(3L)Δ130 male as control females. However, these females lay very few eggs after mating, in contrast to control females. If the females that were mated to Acp70A0/Df(3L)Δ130 males are then tested for receptivity towards a second naive male after being allowed to lay eggs for 48 hours, they mate again at a high frequency and do not actively reject the second male, in contrast to control females tested under the same conditions.

Acp70A0 males fail to significantly induce feeding in females after mating. They exhibit courtship amd mating rates similar to those of controls and successfully fertilise females.

Females mated to Acp70A0 males exhibit only short term post-mating responses (PMR, e.g. increase in egg laying and reduction in receptivity). After two days the PMR are lacking, while in wild-type controls PMRs last for over 5 days.

Females mated with Acp70A0/Df(3L)Δ130 males show only a slight increase in oviposition on day 1 after mating and on the following days, the oviposition rate does not differ from the rate of unmated females (in contrast to females mated to wild-type males which show a considerable increase in oviposition in the first 4 days after mating). Females mated to Acp70A0/Df(3L)Δ130 males show a strong reduction of receptivity only 4 hours after copulation. 12 and 24 hours after copulation, receptivity is more than 60%. After 48 hours, receptivity has increased to virgin levels. Sperm of Acp70A0/Df(3L)Δ130 males are motile and are transferred and stored correctly.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Females expressing Scer\GAL4repo>moodyGD709 mated with SP0 mutant males behave very much like virgins.

Females expressing Scer\GAL4repo>moodyKK100674 mated with SP0 mutant males behave very much like virgins.

Df(1)JC70/Df(1)Exel6234 females expressing Scer\GAL4repo>moodyGD709 mated with SP0 mutant males behave very much like virgins.

Df(1)JC70/Df(1)Exel6234 females expressing Scer\GAL4repo>moodyKK100674 mated with SP0 mutant males behave very much like virgins.

Wild-type mated females often excrete fecal deposits which are extremely concentrated, referred to as reproductive oblong deposits (RODs), compared to the fecal deposits of wild-type males and wild-type virgin females. ovoD1 females which have been mated to Acp70A0/Df(3L)Δ130 males do not produce RODs.

Female flies expressing rprScer\UAS.C under the control of Scer\GAL4Ilp2.215-3 do not show a significantly reduced re-mating rate 5 hours after mating to Acp70A0 flies.

Female Ilp21 flies show a significantly reduced re-mating rate 5 hours after mating to Acp70A0 flies.

Female Ilp31 flies do not show a significantly reduced re-mating rate 5 hours after mating to Acp70A0 flies.

Female Df(3L)Ilp2-3 flies show a significantly reduced re-mating rate 5 hours after mating to Acp70A0 flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Not rescued by

SP0 is not rescued by SPQQ

SP0 is not rescued by SPΔ2-7

Comments

Expression of Acp70A+t1893 rescues egg-laying rate and post-mating behavioral responses and receptivity to approximately wild-type levels.

Expression of Acp70AMip fails to rescue egg-laying rate and post-mating behavioral responses and receptivity to approximately wild-type levels in wild-type female flies that have been mated to Acp70A0/Df(3L)Δ130 mutant males.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (22)