Allele Dmel\Acp70A⁰

General Information
Symbol	Dmel\Acp70A⁰	Species	D. melanogaster
Name		FlyBase ID	FBal0150995
Feature type	allele	Associated gene	Dmel\Acp70A
Also Known As	SP⁰

Allele class
Mutagen	I-CreI endonuclease, ends-in gene targeting
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ends-in gene targeting (Liu and Kubli, 2003) I-CreI endonuclease (Liu and Kubli, 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Dp(3;3)Acp⁺⁰ (Liu and Kubli, 2003)
Nature of the lesion	Statement Reference Reduction of the duplication of the Acp70A genes present in Dp(3;3)Acp⁺⁰, using Crei\I-CreI recombinase. (Liu and Kubli, 2003)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective \| female (with Df(3L)Δ130) (Yapici et al., 2008) courtship behavior defective (Kim et al., 2010) courtship behavior defective \| female (with Df(3L)Δ130) (Yapici et al., 2008) feeding behavior defective \| female limited (Carvalho et al., 2006) mating behavior defective \| female (with Df(3L)Δ130) (Yang et al., 2009)
Phenotype Manifest In
	spermatozoon (Peng et al., 2005) spermatozoon, with Acp70A^QQ (Peng et al., 2005) spermatozoon, with Acp70A^Δ2-7 (Peng et al., 2005)
Detailed Description
	Statement Reference Acp70A[0]/Df(3L)Δ130 males court females marginally significantly more than Acp70A[+] males. (Fricke et al., 2010) Wild-type female flies mated to Acp70A[0]/Df(3L)Δ130 mutant males exhibit a severe reduction in egg-laying per female 48 hours after mating. Approximately 80% of these females copulate again within 1 hour after initial mating and appear receptive to mating. Acp70A[0] heterozygous males elicit a robust post-mating response from wild-type females. (Kim et al., 2010) Females previously mated to Acp70A[0]/Df(3L)Δ130 males remate more frequently and sooner than do females mated to control males. Females mated to Acp70A[0]/Df(3L)Δ130 males lay significantly fewer eggs in the period between their first and second matings than females mated to control males. (Fricke et al., 2009) Females mated to Acp70A[0]/Df(3L)Δ130 males do not show a reduction in receptivity to courting males and do not increase their egg-laying rate, in contrast to wild-type mated females. (Yang et al., 2009) Wild-type virgin females show the same receptivity towards a naive Acp70A⁰/Df(3L)Δ130 male as control females. However, these females lay very few eggs after mating, in contrast to control females. If the females that were mated to Acp70A⁰/Df(3L)Δ130 males are then tested for receptivity towards a second naive male after being allowed to lay eggs for 48 hours, they mate again at a high frequency and do not actively reject the second male, in contrast to control females tested under the same conditions. (Yapici et al., 2008) Acp70A⁰ males fail to significantly induce feeding in females after mating. They exhibit courtship amd mating rates similar to those of controls and successfully fertilise females. (Carvalho et al., 2006) Females mated to Acp70A⁰ males exhibit only short term post-mating responses (PMR, e.g. increase in egg laying and reduction in receptivity). After two days the PMR are lacking, while in wild-type controls PMRs last for over 5 days. (Peng et al., 2005) Females mated with Acp70A⁰/Df(3L)Δ130 males show only a slight increase in oviposition on day 1 after mating and on the following days, the oviposition rate does not differ from the rate of unmated females (in contrast to females mated to wild-type males which show a considerable increase in oviposition in the first 4 days after mating). Females mated to Acp70A⁰/Df(3L)Δ130 males show a strong reduction of receptivity only 4 hours after copulation. 12 and 24 hours after copulation, receptivity is more than 60%. After 48 hours, receptivity has increased to virgin levels. Sperm of Acp70A⁰/Df(3L)Δ130 males are motile and are transferred and stored correctly. (Liu and Kubli, 2003)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Wild-type mated females often excrete fecal deposits which are extremely concentrated, referred to as reproductive oblong deposits (RODs), compared to the fecal deposits of wild-type males and wild-type virgin females. ovo[D1] females which have been mated to Acp70A[0]/Df(3L)Δ130 males do not produce RODs. (Cognigni et al., 2011) Female flies expressing rpr[Scer\UAS.C] under the control of Scer\GAL4[Ilp2.215-3] do not show a significantly reduced re-mating rate 5 hours after mating to Acp70A[0] flies. Female Ilp2[1] flies show a significantly reduced re-mating rate 5 hours after mating to Acp70A[0] flies. Female Ilp3[1] flies do not show a significantly reduced re-mating rate 5 hours after mating to Acp70A[0] flies. Female Df(3L)Ilp2-3 flies show a significantly reduced re-mating rate 5 hours after mating to Acp70A[0] flies. (Wigby et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Acp70A⁰/Df(3L)Δ130 is rescued by Acp70A^+t1893 (Kim et al., 2010)
Not rescued by	Acp70A⁰/Df(3L)Δ130 is not rescued by Acp70A^Mip (Kim et al., 2010) Acp70A⁰ is not rescued by Acp70A^QQ (Peng et al., 2005) Acp70A⁰ is not rescued by Acp70A^Δ2-7 (Peng et al., 2005)
Comments	Expression of Acp70A[+t1893] rescues egg-laying rate and post-mating behavioral responses and receptivity to approximately wild-type levels. Expression of Acp70A[Mip] fails to rescue egg-laying rate and post-mating behavioral responses and receptivity to approximately wild-type levels in wild-type female flies that have been mated to Acp70A[0]/Df(3L)Δ130 mutant males. (Kim et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Acp70A⁰ SP⁰ (Peng et al., 2005, Peng et al., 2005, Liu and Kubli, 2003, Carvalho et al., 2006, Yang et al., 2009, Yapici et al., 2008, Fricke et al., 2009, Fricke et al., 2010, Wigby et al., 2011, Cognigni et al., 2011, Kim et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Keleman et al., 2012, Nature 489(7414): 145--149 Dopamine neurons modulate pheromone responses in Drosophila courtship learning. [FBrf0219398] Cognigni et al., 2011, Cell Metab. 13(1): 92--104 Enteric neurons and systemic signals couple nutritional and reproductive status with intestinal homeostasis. [FBrf0212594] Wigby et al., 2011, Proc. Biol. Sci. 278(1704): 424--431 Insulin signalling regulates remating in female Drosophila. [FBrf0212666] Fricke et al., 2010, J. Evol. Biol. 23(1): 157--165 Female nutritional status determines the magnitude and sign of responses to a male ejaculate signal in Drosophila melanogaster. [FBrf0209664] Kim et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(14): 6520--6525 MIPs are ancestral ligands for the sex peptide receptor. [FBrf0210504] Fricke et al., 2009, J. Evol. Biol. 22(2): 275--286 The benefits of male ejaculate sex peptide transfer in Drosophila melanogaster. [FBrf0207112] Yang et al., 2009, Neuron 61(4): 519--526 Control of the postmating behavioral switch in Drosophila females by internal sensory neurons. [FBrf0207501] Yapici et al., 2008, Nature 451(7174): 33--37 A receptor that mediates the post-mating switch in Drosophila reproductive behaviour. [FBrf0202765] Carvalho et al., 2006, Curr. Biol. 16(7): 692--696 Allocrine modulation of feeding behavior by the Sex Peptide of Drosophila. [FBrf0190002] Peng et al., 2005, Curr. Biol. 15(18): 1690--1694 Drosophila Sex-Peptide Stimulates Female Innate Immune System after Mating via the Toll and Imd Pathways. [FBrf0187323] Peng et al., 2005, Curr. Biol. 15(3): 207--213 Gradual release of sperm bound sex-peptide controls female postmating behavior in Drosophila. [FBrf0183825] Liu and Kubli, 2003, Proc. Natl. Acad. Sci. U.S.A. 100(17): 9929--9933 Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogaster. [FBrf0161518]

Allele Dmel\Acp70A0

Allele Dmel\Acp70A⁰