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General Information
Symbol
Dmel\wtsUAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0151322
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-wts, UAS-Myc:Wts, UAS-Myc-Wts
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS sequences drive expression of wts tagged with Tag:MYC.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF causes a reduction in eye size.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Phenotype Manifest In
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Flies expressing wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL rescue the lethality and third instar larval wing disc overgrowth phenotypes seen in ft8/ftG-rv mutants, but the wing hair phenotypes are still present. Rather than pointing distally as in wild type, the wing hairs in these flies are misoriented and swirling patterns can be observed. A strong planar cell polarity defect is also seen in the abdominal hairs and the wing anterior-posterior crossvein distance is reduced. Expression of ftT:SV5\V5 fully rescues the planar cell polarity defects seen in ft8/ftG-rv mutant wings and abdomens expressing wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL. The reduced anterior-posterior crossvein distance phenotype is also suppressed. Flies expressing ftΔEGF.T:SV5\V5 (co-expressed with wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL) in a ft8/ftG-rv mutant background do not exhibit any wing phenotypes compared to flies expressing wtsScer\UAS.T:Hsap\MYC alone. Expression of ftΔICD.T:SV5\V5 partially rescues the planar cell polarity defects seen in ft8/ftG-rv mutant wings and abdomens expressing wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL. The reduction in anterior-posterior crossvein distance appears to be partially rescued, although crossveins are often incomplete, making it difficult to calculate. The abdominal and wing hair polarity defects are also partially suppressed.
Expression of wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act.PU does not suppress the head overgrowth seen in mopsfv3 Df(3L)H99 mutant clones (generated in a minute background).
Expression of TaoKK108458 has no effect on the reduction in eye size seen when wtsScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PF.
The lethality and overgrowth phenotypes of ftG-rv/ft8 animals are rescued by Scer\GAL4tub.PU-mediated overexpression of wtsScer\UAS.T:Hsap\MYC. However, these animals still show obvious planar cell polarity phenotypes in multiple tissues. The lethality and overgrowth phenotypes of dco3 animals are rescued by Scer\GAL4tub.PU-mediated overexpression of wtsScer\UAS.T:Hsap\MYC, resulting in animals that, aside from some mild wing vein phenotypes, are indistinguishable from wild type animals over-expressing wtsScer\UAS.T:Hsap\MYC.
Co-expression of ykiS111A.S250A.Scer\UAS.T:SV5\V5, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in flies with very small eyes. Co-expression of ykiS168A.Scer\UAS.T:SV5\V5, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in flies with enlarged eyes. Co-expression of ykiS111A.S168A.Scer\UAS.T:SV5\V5, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in flies with greatly enlarged eyes (but less so than in the absence of wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG). Co-expression of ykiS168A.S250A.Scer\UAS.T:SV5\V5, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in flies with greatly enlarged eyes (but less so than in the absence of wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG). Co-expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in flies with enlarged and highly folded eyes. Clones in the wing disc co-expressing ykiS111A.S250A.Scer\UAS.T:SV5\V5, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act5C.PI do not survive well. Large round clones are still observed in the wing disc when ykiS168A.Scer\UAS.T:SV5\V5 is co-expressed with wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act5C.PI in the clones. Co-expression of exScer\UAS.cBa in clones expressing ykiS168A.Scer\UAS.T:SV5\V5, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act5C.PI decreases the size and number of clones recovered in the wing disc. Clones co-expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 exScer\UAS.cBa, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act5C.PI in the wing disc are large and round.
Clones of cells in the wing or eye disc in which wtsScer\UAS.T:Hsap\MYC, hpoScer\UAS.T:Zzzz\FLAG and exScer\UAS.cBa are over-expressed together with ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 generally overgrow compared to control clones, but can be less overgrown than ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5-expressing clones alone. This is most evident in the wing pouch. The overgrowth and folding of the eye disc induced by Scer\GAL4GMR.PU-mediated expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is largely suppressed by co-expression of wtsScer\UAS.T:Hsap\MYC, hpoScer\UAS.T:Zzzz\FLAG and exScer\UAS.cBa. The overgrowth and folding of the eye disc induced by Scer\GAL4GMR.PU-mediated expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is not suppressed by co-expression of wtsScer\UAS.T:Hsap\MYC alone, nor by co-expression of wtsScer\UAS.T:Hsap\MYC and exScer\UAS.cBa together. The overgrowth and folding of the eye disc induced by Scer\GAL4GMR.PU-mediated expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is partly suppressed by co-expression of wtsScer\UAS.T:Hsap\MYC, hpoScer\UAS.T:Zzzz\FLAG together.
Clones co-expressing hpoScer\UAS.T:Zzzz\FLAG and wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PI are not recovered in the wing disc. Clones co-expressing hpoScer\UAS.T:Zzzz\FLAG, ykiS168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 and wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PI are not recovered in the wing disc.
Xenogenetic Interactions
Statement
Reference
Expression of ft::Hsap\FAT4T:SV5\V5 partially rescues the planar cell polarity defects seen in ft8/ftG-rv mutant wings expressing wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL. The abdominal hair defects are completely rescued and the reduction in anterior-posterior crossvein distance is partially suppressed.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
wtsScer\UAS.T:Hsap\MYC
wtsUAS.Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (15)