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General Information
Symbol
Dmel\vap2
Species
D. melanogaster
Name
FlyBase ID
FBal0151357
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Deletion of 239 base pairs starting from position 999 (by the numbering of cDNA AJ012609). This deletion removes a splice donor site at 1099 bp. The resulting predicted protein, from a transcript using a splice donor site at 907 bp, is lacking amino acids 206-269.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    vap2 homozygous adults show significantly decreased body-mass when raised in standard diet, but not in poor diet, as compared to controls. These adults are hypersensitive to acute food deprivation, but not if fed 15% sucrose only.

    The maximum lifespan of vap2 homozygous flies is 25 days, compared to 90 days for wild-type controls. Vacuolisation is apparent in brains vap2 homozygous flies by 15 days of age.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressed by
    Statement
    Reference

    vap2 has short lived | calorie restriction conditional phenotype, suppressible by spri6G1/spri6G1

    vap2 has short lived | calorie restriction conditional phenotype, suppressible by spri6G1/spri[+]

    vap2 has abnormal neuroanatomy phenotype, suppressible by Rab5[+]/Rab54

    vap2 has abnormal neuroanatomy phenotype, suppressible by Rab52/Rab5[+]

    vap2 has abnormal neuroanatomy phenotype, suppressible by spri6G1/spri[+]

    Other
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference
    Suppressed by
    Statement
    Reference

    vap2 has adult brain phenotype, suppressible by spri6G1

    vap2 has adult brain phenotype, suppressible by Rab5[+]/Rab54

    vap2 has adult brain phenotype, suppressible by Rab52/Rab5[+]

    vap2 has adult optic lobe | adult stage phenotype, suppressible | partially by Egfrf3/Egfr[+]

    vap2 has adult optic lobe | adult stage phenotype, suppressible | partially by drkk02401/drk[+]

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    vap2/vap2, spri6G1/spri6G1 and vap2/+, spri6G1/spri6G1 double mutants show the same increased resistance to acute food deprivation as spri6G1/spri6G1 single mutants, instead of the hypersensitivity observed in vap2/vap2 single mutants.

    A homozygous spri6G1 mutant background significantly suppresses the vap2 neurodegenerative phenotype. Weaker suppression is seen in a spri6G1 heterozygous background.

    A heterozygous Rab54 mutant background suppresses the vap2 neurodegenerative phenotype.

    A heterozygous Rab52 mutant background significantly suppresses the vap2 neurodegenerative phenotype.

    The neurodegenerative phenotype of 18 day old vap2 homozygous flies is dominantly enhanced by styS73 and by rhohs.PSt or EgfrWT.hs under heat shock conditions or Ras85DScer\UAS.cKa; Scer\GAL4Appl.G1a. (Assayed by the percentage of vacuolisation in the optic lobes in 18 day old adults). The neurodegenerative phenotype of 18 day old vap2 homozygous flies is largely suppressed by Egfrf3/+ or drkk02401/+, or by reduction of Egfr levels in adulthood using the temperature sensitive allele Egfrtsla/+. (Assayed by the percentage of vacuolisation in the optic lobes in 18 day old adults). The genetic combination: vap2; Ras85DV12.C40.Scer\UAS; Scer\GAL4Appl.G1a is lethal.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments

    Expression of vapSH2mut32.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav.PU fails to rescue the neurodegeneration phenotype seen in vap2 mutant flies.

    Expression of vapScer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav.PU rescues the neurodegeneration phenotype seen in vap2 mutant flies.

    Expression of vapSH23mut2.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav.PU rescues the neurodegeneration phenotype seen in vap2 mutant flies.

    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (3)