Allele Dmel\tlk^Scer\UAS.cCa

General Information
Symbol	Dmel\tlk^Scer\UAS.cCa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Carrera	FlyBase ID	FBal0151380
Feature type	allele	Associated gene	Dmel\tlk

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Carrera et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of a full length cDNA of tlk. (Carrera et al., 2003)
Carried in construct	P{UAS-tlk.C} (Carrera et al., 2003, Li et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death, with Scer\GAL4^arm.PS (Carrera et al., 2003) increased cell death, with Scer\GAL4^Bx-MS1096 (Carrera et al., 2003) increased cell death, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Carrera et al., 2003) lethal \| larval stage \| heat sensitive, with Scer\GAL4^hs.2sev (Carrera et al., 2003) mitotic cell cycle defective, with Scer\GAL4^{bcd3.1.T:Scer\GCN4} (Li et al., 2009) mitotic cell cycle defective, with Scer\GAL4^GMR.PF (Li et al., 2009)
Phenotype Manifest In
	embryonic/larval salivary gland, with Scer\GAL4^hs.2sev (Carrera et al., 2003) eye, with Scer\GAL4^ey.PH (Carrera et al., 2003) larval salivary gland & nuclear chromosome, with Scer\GAL4^hs.2sev (Carrera et al., 2003) macrochaeta, with Scer\GAL4^hs.2sev (Carrera et al., 2003)
Detailed Description
	Statement Reference Overexpression of tlk[Scer\UAS.cCa] under the control of Scer\GAL4[bcd3.1.T:Scer\GCN4] includes a delayed progression of mitotic events, which is manifest as several observable features. The fraction of embryos at prophase is increased compared to wild-type, while the percentage in interphase is reduced. Patches of nuclei with delayed chromosome congression are seen in 30% of the metaphase embryos. Some nuclei exhibit delayed chromosome segregation with chromosome bridges in half of anaphase embryos. Neither the length nor the density of most mitotic spindles at metaphase are substantially affected by tlk[Scer\UAS.cCa] overexpression. tlk[Scer\UAS.cCa] overexpression in the eye disc under the control of Scer\GAL4[GMR.PF] results in cells in domain I being retained at interphase and many cells in domains II and III being retained at M-phase. This should be compared to the fact that most M-phase cells appear in domain I in control eye discs. (Li et al., 2009) Expression of tlk^Scer\UAS.cCa driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, Scer\GAL4^arm.PS or Scer\GAL4^Bx-MS1096 in wing imaginal discs results in fewer and enlarged cells. Expression of tlk^Scer\UAS.cCa when driven by Scer\GAL4^ey.PH leads to a mild rough eye phenotype. Expression of tlk^Scer\UAS.cCa driven by a 2 hour heatshock (driven by Scer\GAL4^hs.2sev) at any time during larval development is lethal. When expressed at low enough levels for the animals to survive to adulthood, the only phenotype seen is greatly reduced larval salivary glands, though the cell number is not significantly reduced. Severe defects are seen in the morphology of the salivary gland nuclei, chromosomes are thinner than wild-type suggesting a defect in endoreplication. Chromosomes are grossly disrupted, losing their typical banding pattern. If these animals develop to adulthood a number of macrochaetae are seen to be missing, and the sex ratio of those adults is skewed towards females. (Carrera et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference tlk^Scer\UAS.cCa, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF is a suppressor of mitotic cell cycle defective phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, mars^EP2477 (Li et al., 2009)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^ey.PH, tlk^Scer\UAS.cCa has eye phenotype, enhanceable by asf1^Scer\UAS.cMa (Carrera et al., 2003) Scer\GAL4^hs.2sev, tlk^Scer\UAS.cCa has embryonic/larval salivary gland phenotype, enhanceable by asf1^Scer\UAS.cMa (Carrera et al., 2003) Scer\GAL4^hs.2sev, tlk^Scer\UAS.cCa has larval salivary gland & nuclear chromosome phenotype, enhanceable by asf1^Scer\UAS.cMa (Carrera et al., 2003)
Enhancer of
Statement Reference tlk^Scer\UAS.cCa is an enhancer of embryonic/larval salivary gland phenotype of Scer\GAL4^hs.2sev, asf1^Scer\UAS.cMa (Carrera et al., 2003) tlk^Scer\UAS.cCa is an enhancer of eye phenotype of Scer\GAL4^ey.PH, asf1^Scer\UAS.cMa (Carrera et al., 2003) tlk^Scer\UAS.cCa is an enhancer of salivary gland & nucleus & larva phenotype of Scer\GAL4^hs.2sev, asf1^Scer\UAS.cMa (Carrera et al., 2003)
Other
Statement Reference Scer\GAL4^ey.PH, asf1^Scer\UAS.cMa, tlk^Scer\UAS.cCa has head phenotype (Carrera et al., 2003, Carrera et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of mars[EP2477] and tlk[Scer\UAS.cCa] in the eye disc under the control of Scer\GAL4[GMR.PF] results in the number of M-phase cells in domain III being reduced to a level close to that of wild-type, showing that metaphase arrest induced by mars[EP2477] overexpression is suppressed by tlk[Scer\UAS.cCa] overexpression. (Li et al., 2009) Co-expression of tlk^Scer\UAS.cCa and asf1^Scer\UAS.cMa driven by Scer\GAL4^ey.PH leads to a massive reduction in head and eye structures, creating a 'pin-head' phenotype. When tlk^Scer\UAS.cCa and asf1^Scer\UAS.cMa are driven by Scer\GAL4^hs.2sev in conditions that allow animals to survive to adulthood, the salivary gland defects are enhanced. (Carrera et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	tlk^Scer\UAS.cCa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Carrera (Carrera et al., 2003)
Secondary FlyBase IDs

References ( 2 )
Research paper	Li et al., 2009, J. Biomed. Sci. 16: 51 mars and tousled-like kinase act in parallel to ensure chromosome fidelity in Drosophila. [FBrf0208159] Carrera et al., 2003, Genes Dev. 17(20): 2578--2590 Tousled-like kinase functions with the chromatin assembly pathway regulating nuclear divisions. [FBrf0167605]

Allele Dmel\tlkScer\UAS.cCa

Allele Dmel\tlk^Scer\UAS.cCa