FlyBase Allele Report: Dmel\Tlk[UAS.cCa]

General Information

Symbol

Dmel\Tlk^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Carrera

FlyBase ID

FBal0151380

Feature type

allele

Associated gene

Dmel\Tlk

Associated Insertion(s)

Carried in Construct

P{UAS-Tlk.C}

Key Links

Transgenic product class

cDNA

(Carrera et al., 2003)

wild_type

(Carrera et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Carrera et al., 2003)

wild_type

(Carrera et al., 2003)

Mutagen

in vitro construct

(Carrera et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Tlk.C}

Cytology

Description

UASt sequences drive expression of a full length cDNA of Tlk.

(Carrera et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tlk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^GCN4.bcd3.1

(Li et al., 2009)

abnormal mitotic cell cycle, with Scer\GAL4^GMR.PF

(Li et al., 2009)

abnormal size | adult stage, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

decreased cell number | larval stage, with Scer\GAL4^arm.PS

(Carrera et al., 2003)

decreased cell number | larval stage, with Scer\GAL4^Bx-MS1096

(Carrera et al., 2003)

decreased cell number | larval stage, with Scer\GAL4^hs.PH

(Carrera et al., 2003)

increased cell death, with Scer\GAL4^arm.PS

(Carrera et al., 2003)

increased cell death, with Scer\GAL4^Bx-MS1096

(Carrera et al., 2003)

increased cell death, with Scer\GAL4^{VP16.mat.αTub67C}

(Carrera et al., 2003)

increased cell size | larval stage, with Scer\GAL4^arm.PS

(Carrera et al., 2003)

increased cell size | larval stage, with Scer\GAL4^Bx-MS1096

(Carrera et al., 2003)

increased cell size | larval stage, with Scer\GAL4^hs.PH

(Carrera et al., 2003)

lethal - all die before end of larval stage | heat sensitive, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

some die during larval stage | heat sensitive, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

Phenotype Manifest In

embryonic/larval salivary gland, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

eye, with Scer\GAL4^ey.PH

(Carrera et al., 2003)

larval salivary gland & nuclear chromosome, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

macrochaeta, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

Detailed Description

Statement

Reference

Overexpression of tlk^Scer\UAS.cCa under the control of Scer\GAL4^{bcd3.1.T:Scer\GCN4} includes a delayed progression of mitotic events, which is manifest as several observable features. The fraction of embryos at prophase is increased compared to wild-type, while the percentage in interphase is reduced. Patches of nuclei with delayed chromosome congression are seen in 30% of the metaphase embryos. Some nuclei exhibit delayed chromosome segregation with chromosome bridges in half of anaphase embryos. Neither the length nor the density of most mitotic spindles at metaphase are substantially affected by tlk^Scer\UAS.cCa overexpression.

tlk^Scer\UAS.cCa overexpression in the eye disc under the control of Scer\GAL4^GMR.PF results in cells in domain I being retained at interphase and many cells in domains II and III being retained at M-phase. This should be compared to the fact that most M-phase cells appear in domain I in control eye discs.

(Li et al., 2009)

Expression of tlk^Scer\UAS.cCa driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, Scer\GAL4^arm.PS or Scer\GAL4^Bx-MS1096 in wing imaginal discs results in fewer and enlarged cells. Expression of tlk^Scer\UAS.cCa when driven by Scer\GAL4^ey.PH leads to a mild rough eye phenotype. Expression of tlk^Scer\UAS.cCa driven by a 2 hour heatshock (driven by Scer\GAL4^hs.2sev) at any time during larval development is lethal. When expressed at low enough levels for the animals to survive to adulthood, the only phenotype seen is greatly reduced larval salivary glands, though the cell number is not significantly reduced. Severe defects are seen in the morphology of the salivary gland nuclei, chromosomes are thinner than wild-type suggesting a defect in endoreplication. Chromosomes are grossly disrupted, losing their typical banding pattern. If these animals develop to adulthood a number of macrochaetae are seen to be missing, and the sex ratio of those adults is skewed towards females.

(Carrera et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, Tlk^UAS.cCa has abnormal size | adult stage phenotype, enhanceable by asf1^UAS.cMa/Scer\GAL4^ey.PH

(Carrera et al., 2003)

Suppressor of

Statement

Reference

Tlk^UAS.cCa/Scer\GAL4^GMR.PF is a suppressor of abnormal mitotic cell cycle phenotype of Scer\GAL4^GMR.PF, mars^EP2477

(Li et al., 2009)

Other

Statement

Reference

Scer\GAL4^ey.PH, Tlk^UAS.cCa, asf1^UAS.cMa has abnormal size | adult stage phenotype

(Carrera et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, Tlk^UAS.cCa has larval salivary gland & nuclear chromosome phenotype, enhanceable by asf1^UAS.cMa

(Carrera et al., 2003)

Scer\GAL4^hs.2sev, Tlk^UAS.cCa has embryonic/larval salivary gland phenotype, enhanceable by asf1^UAS.cMa

(Carrera et al., 2003)

Scer\GAL4^ey.PH, Tlk^UAS.cCa has eye phenotype, enhanceable by asf1^UAS.cMa

(Carrera et al., 2003)

Enhancer of

Statement

Reference

Tlk^UAS.cCa is an enhancer of salivary gland & nucleus & larva phenotype of Scer\GAL4^hs.2sev, asf1^UAS.cMa

(Carrera et al., 2003)

Tlk^UAS.cCa is an enhancer of embryonic/larval salivary gland phenotype of Scer\GAL4^hs.2sev, asf1^UAS.cMa

(Carrera et al., 2003)

Tlk^UAS.cCa is an enhancer of eye phenotype of Scer\GAL4^ey.PH, asf1^UAS.cMa

(Carrera et al., 2003)

Other

Statement

Reference

Scer\GAL4^ey.PH, Tlk^UAS.cCa, asf1^UAS.cMa has head phenotype

(Carrera et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of mars^EP2477 and tlk^Scer\UAS.cCa in the eye disc under the control of Scer\GAL4^GMR.PF results in the number of M-phase cells in domain III being reduced to a level close to that of wild-type, showing that metaphase arrest induced by mars^EP2477 overexpression is suppressed by tlk^Scer\UAS.cCa overexpression.

(Li et al., 2009)

Co-expression of tlk^Scer\UAS.cCa and asf1^Scer\UAS.cMa driven by Scer\GAL4^ey.PH leads to a massive reduction in head and eye structures, creating a 'pin-head' phenotype. When tlk^Scer\UAS.cCa and asf1^Scer\UAS.cMa are driven by Scer\GAL4^hs.2sev in conditions that allow animals to survive to adulthood, the salivary gland defects are enhanced.

(Carrera et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Tlk^Scer\UAS.cCa

Tlk^UAS.cCa

tlk^Scer\UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Carrera

(Carrera et al., 2003)

Secondary FlyBase IDs

References (3)

Report Sections

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