|Name||Saccharomyces cerevisiae UAS construct a of Carrera||FlyBase ID||FBal0151380|
|Feature type||allele||Associated gene||Dmel\tlk|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Overexpression of tlk[Scer\UAS.cCa] under the control of Scer\GAL4[bcd3.1.T:Scer\GCN4] includes a delayed progression of mitotic events, which is manifest as several observable features. The fraction of embryos at prophase is increased compared to wild-type, while the percentage in interphase is reduced. Patches of nuclei with delayed chromosome congression are seen in 30% of the metaphase embryos. Some nuclei exhibit delayed chromosome segregation with chromosome bridges in half of anaphase embryos. Neither the length nor the density of most mitotic spindles at metaphase are substantially affected by tlk[Scer\UAS.cCa] overexpression. tlk[Scer\UAS.cCa] overexpression in the eye disc under the control of Scer\GAL4[GMR.PF] results in cells in domain I being retained at interphase and many cells in domains II and III being retained at M-phase. This should be compared to the fact that most M-phase cells appear in domain I in control eye discs.
Expression of tlkScer\UAS.cCa driven by Scer\GAL4mat.αTub67C.T:Hsim\VP16, Scer\GAL4arm.PS or Scer\GAL4Bx-MS1096 in wing imaginal discs results in fewer and enlarged cells. Expression of tlkScer\UAS.cCa when driven by Scer\GAL4ey.PH leads to a mild rough eye phenotype. Expression of tlkScer\UAS.cCa driven by a 2 hour heatshock (driven by Scer\GAL4hs.2sev) at any time during larval development is lethal. When expressed at low enough levels for the animals to survive to adulthood, the only phenotype seen is greatly reduced larval salivary glands, though the cell number is not significantly reduced. Severe defects are seen in the morphology of the salivary gland nuclei, chromosomes are thinner than wild-type suggesting a defect in endoreplication. Chromosomes are grossly disrupted, losing their typical banding pattern. If these animals develop to adulthood a number of macrochaetae are seen to be missing, and the sex ratio of those adults is skewed towards females.
|Phenotype Manifest In|
Scer\GAL4hs.2sev, tlkScer\UAS.cCa has embryonic/larval salivary gland phenotype, enhanceable by asf1Scer\UAS.cMa
tlkScer\UAS.cCa is an enhancer of embryonic/larval salivary gland phenotype of Scer\GAL4hs.2sev, asf1Scer\UAS.cMa
Co-expression of mars[EP2477] and tlk[Scer\UAS.cCa] in the eye disc under the control of Scer\GAL4[GMR.PF] results in the number of M-phase cells in domain III being reduced to a level close to that of wild-type, showing that metaphase arrest induced by mars[EP2477] overexpression is suppressed by tlk[Scer\UAS.cCa] overexpression.
Co-expression of tlkScer\UAS.cCa and asf1Scer\UAS.cMa driven by Scer\GAL4ey.PH leads to a massive reduction in head and eye structures, creating a 'pin-head' phenotype. When tlkScer\UAS.cCa and asf1Scer\UAS.cMa are driven by Scer\GAL4hs.2sev in conditions that allow animals to survive to adulthood, the salivary gland defects are enhanced.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
Saccharomyces cerevisiae UAS construct a of Carrera
|Secondary FlyBase IDs|
|References ( 2 )|