Allele Dmel\stnA^AB.Scer\UAS

General Information
Symbol	Dmel\stnA^AB.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0151406
Feature type	allele	Associated gene	Dmel\stnA

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Estes et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a dicistronic "stn" cDNA which encodes both stnA and stnB proteins. The cDNA contains only 211bp of the 3' untranslated sequence. (Estes et al., 2003)
Carried in construct	P{UAS-stnAB.E} (Estes et al., 2003, Pinto and Krishnan, 2009.2.19)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Scer\GAL4^elav-C155, stnA¹, stnA^AB.Scer\UAS, stnB¹, stnB^AB.Scer\UAS has viable phenotype (Estes et al., 2003, Estes et al., 2003) Scer\GAL4^elav-C155, stnA¹², stnA^AB.Scer\UAS, stnB¹², stnB^AB.Scer\UAS has viable phenotype (Estes et al., 2003, Estes et al., 2003) Scer\GAL4^elav-C155, stnA¹⁵, stnA^AB.Scer\UAS, stnB¹⁵, stnB^AB.Scer\UAS has viable phenotype (Estes et al., 2003) Scer\GAL4^Mhc.PW, stnA¹², stnA^AB.Scer\UAS, stnB¹², stnB^AB.Scer\UAS has lethal phenotype (Estes et al., 2003, Estes et al., 2003) Scer\GAL4^shi.PS, stnA¹, stnA^AB.Scer\UAS, stnB¹, stnB^AB.Scer\UAS has viable phenotype (Estes et al., 2003, Estes et al., 2003) Scer\GAL4^shi.PS, stnA¹², stnA^AB.Scer\UAS, stnB¹², stnB^AB.Scer\UAS has viable phenotype (Estes et al., 2003, Estes et al., 2003)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments	Expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^shi.PS rescues the lethality of stnA¹ stnB¹/Y ("stn^8P1") animals. Expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^shi.PS rescues the lethality of stnA¹² stnB¹²/Y ("stn^13-120") animals. Expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of Scer\GAL4^Mhc.PW does not rescue the lethality of stnA¹² stnB¹²/Y ("stn^13-120") animals. Expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of Scer\GAL4^elav-C155 rescues the mean excitatory junction potential (EJP) amplitude at the neuromuscular junction to wild-type in stnA¹ stnB¹ ("stn^8P1") larvae. Expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of Scer\GAL4^elav-C155 rescues the mean excitatory junction potential (EJP) amplitude at the neuromuscular junction to wild-type in stnA¹⁵ stnB¹⁵ ("stn^C") larvae. The extra "satellite" bouton phenotype seen at the neuromuscular junction of stnA¹ stnB¹ ("stn^8P1") larvae is rescued by expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of Scer\GAL4^elav-C155. (Estes et al., 2003)
Stocks ( 2 )
Bloomington	26790 y¹ w^*; P{UAS-stnAB.E}2 9655 y¹ w^67c23; P{UAS-stnAB.E}1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	stnA^AB.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Estes et al., 2003, Genetics 165(1): 185--196 Functional dissection of a eukaryotic dicistronic gene. Transgenic stonedB, but not stonedA, restores normal synaptic properties to Drosophila stoned mutants. [FBrf0162152]
Personal communication to FlyBase	Pinto and Krishnan, 2009.2.19, P{UAS-stnAB.E}2. P{UAS-stnAB.E}2. [FBrf0206664]

Allele Dmel\stnAAB.Scer\UAS

Allele Dmel\stnA^AB.Scer\UAS