Adult females expressing slifUY681 under the control of Scer\GAL4fit.PS show significantly weaker suppression of feeding (on standard food) after pre-feeding with tryptone compared to controls.
Expression of slifUY681, throughout development, under the control of Scer\GAL4ple.PF, but not Scer\GAL4NPF.PU, Scer\GAL4Trh.PB, Scer\GAL4Tdc2.PU, Scer\GAL4ChAT.PU, Scer\GAL4Gad1.3.098, Scer\GAL4Hug.PU or Scer\GAL4386Y, results in a significant reduction in larval food intake, compared to controls; this effect also occurs when a Gal80[ts] transgene is used to restrict expression under the control of Scer\GAL4ple.PF to the 16 hours prior to analysis.
Expression of slifUY681 under the control of Scer\GAL4ple.TH-D', but not Scer\GAL4ple.TH-C, in combination with a Gal80[ts] transgene to restrict expression to the 16 hours prior to analysis, reduces larval food intake and body size, compared to controls.
Central nervous system neuroblasts of fed larvae expressing slifUY681 under the control of Scer\GAL4Cg.PA show a reduction in EdU incorporation compared to controls.
Larvae expressing slifUY681 under the control of Scer\GAL4ppl.PP and raised to the third instar on a standard diet resemble starved wild-type larvae in that lipid droplets aggregate in the fat body. Oenocytes in third instar larvae expressing slifUY681 under the control of Scer\GAL4ppl.PP contain numerous lipid droplets, both in larvae that have been fed on a standard diet or those that have been food-deprived for 14 hours (this contrasts with wild-type larvae, where oenocytes accumulate lipid droplets specifically under starvation conditions).
Expression of slifUY681 under the control of Scer\GAL4Lsp2.PH results in an accumulation of lipid droplets in the oenocytes of fed larvae.
Colored food is present in the gut of Scer\GAL4ppl.PP>slifUY681 animals, showing that these mutants are able to swallow and process food.
When slifUY681 is driven by Scer\GAL4Bx-MS1096 a reduction in size is seen. The animals have phenotypes mimicking amino acid deprivation, exhibiting storage vesicle aggregation in the fat body. When slifUY681 is driven by Scer\GAL4ppl.PP in the fat bodies, despite normal feeding behaviour and processing of food along the digestive tract, larval development is delayed and accompanied by significant growth defects. Growth rate is strongly reduced throughout larval life. When induced at 18oC emerging adults are reduced in size and body weight (54% of wild-type). Stronger induction (at 25oC) provokes a longer developmental delay associated with pupal lethality. Unlike for wild-type flies, a 90% reduction of arginine concentration in the food leads to a dramatic aggravation of the growth defect, with most animals dying as small L2 larvae. This aggravation is not seen after starvation for valine. growth in the salivary glands (a larval endoreduplicating tissue) are less affected than growth in imaginal discs. These larvae also displayed remobilisation of lipid vesicles in fat body cells, as well as a reduction in cell size and endoreduplication levels comparable to the effect of a diet lacking protein.
Flies expressing slifUY681 under the control of Scer\GAL4en-e16E show a significant difference in growth between the posterior compartment of the wing (which expresses Scer\GAL4en-e16E) and the anterior compartment of the wing (internal control).