Allele Dmel\rl^{hs.T:SV40\nls2,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD}

General Information
Symbol	Dmel\rl^{hs.T:SV40\nls2,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD}	Species	D. melanogaster
Name		FlyBase ID	FBal0151602
Feature type	allele	Associated gene	Dmel\rl

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Kumar et al., 2003) in vitro construct - regulatory fusion (Kumar et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: An Hsp70 promoter drives expression of the complete rl coding sequence, followed by amino acids 2-147 of Scer\GAL4 (includes the DNA-binding domain), followed by residues 413-490 of T:Hsim\VP16 (activation domain), followed by a T:Hsim\gD epitope tag. The fusion protein is tagged at the N-terminal end with a nuclear localisation signal (T:SV40\nls2). (Kumar et al., 2003)
Carried in construct	P{hs-rl.NMG} (Kumar et al., 2003)
Tagged with	T:Hsim\gD (Kumar et al., 2003) T:Hsim\VP16 (Kumar et al., 2003) T:Scer\GAL4 (Kumar et al., 2003) T:SV40\nls2 (Kumar et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	rl^{hs.T:SV40\nls2,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD} partially rescues rl¹ (Kumar et al., 2003)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	rl^{hs.T:SV40\nls2,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Kumar et al., 2003, Development 130(16): 3703--3714 Nuclear translocation of activated MAP kinase is developmentally regulated in the developing Drosophila eye. [FBrf0160704]

Allele Dmel\rlhs.T:SV40\nls2,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD

Allele Dmel\rl^{hs.T:SV40\nls2,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD}