FlyBase Allele Report: Dmel\rl[Sem.S.UAS]

General Information

Symbol

Dmel\rl^Sem.S.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0151605

Feature type

allele

Associated gene

Dmel\rl

Associated Insertion(s)

Carried in Construct

P{UAS-rl^Sem.S}

Also Known As

UAS-rl^Sem

Key Links

Transgenic product class

missense_variant

(Rintelen et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Rintelen et al., 2003)

Mutagen

in vitro construct

(Rintelen et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-rl^Sem.S}

Cytology

Description

UAS regulatory sequences drive expression of an activated form of rl.

(Rintelen et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut | somatic clone, with Scer\GAL4^Tub.PU

(Mattila et al., 2018)

eye, with Scer\GAL4^da.G32

(Tipping et al., 2010)

myoblast | increased number | larval stage, with Scer\GAL4^GMR32D05

(Moucaud et al., 2024)

spermatocyte, with Scer\GAL4^VP16.bam

(Azuma et al., 2021)

wing, with Scer\GAL4^da.G32

(Tipping et al., 2010)

wing blade, with Scer\GAL4^salm.EPv

(Ashton-Beaucage et al., 2016)

wing vein, with Scer\GAL4^da.G32

(Tipping et al., 2010)

wing vein | ectopic, with Scer\GAL4^salm.EPv

(Ashton-Beaucage et al., 2016)

wing vein | increased number

(Rintelen et al., 2003)

Detailed Description

Statement

Reference

Expressing rl^Sem.S.UAS under the control of Scer\GAL4^C587 does not induce any obvious L3 spermatogenesis defects.

(La Marca et al., 2019)

Expression of rl^Sem.S.UAS under the control of Scer\GAL4^Tub.PU in MARCM clones leads to a significant increase in midgut cell number when compared to control adults.

(Mattila et al., 2018)

Expression of rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^salm.EPv leads to formation of extra vein material in adult wings.

(Ashton-Beaucage et al., 2016)

Expression of rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 results in the formation of additional vein material, and a smaller overall sized wing is observed.

Expression of rl^{Sem.S.Scer\UAS} in the eye under the control of Scer\GAL4^da.G32 results in mild irregularities in ommatidial pattern.

Flies expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 have a survival rate of 42%.

(Tipping et al., 2010)

Flies carrying rl^{Sem.S.Scer\UAS} have some additional wing veins, even in the absence of an Scer\GAL4 driver.

(Rintelen et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has visible | adult stage phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^KK101471

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has visible | adult stage phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^GD8339

(Ashton-Beaucage et al., 2016)

Scer\GAL4^da.G32, rl^Sem.S.UAS has visible phenotype, enhanceable by krz^S047819/krz[+]

(Tipping et al., 2010)

Scer\GAL4^da.G32, rl^Sem.S.UAS has partially lethal - majority die phenotype, enhanceable by krz^S047819/krz[+]

(Tipping et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, rl^Sem.S.UAS has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^salm.EPv/Usp47^NIG.5486R

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, rl^Sem.S.UAS has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^salm.EPv/Usp47^GD10642

(Ashton-Beaucage et al., 2016)

Scer\GAL4^da.G32, krz^S047819/krz[+], rl^Sem.S.UAS has visible phenotype, suppressible by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Tipping et al., 2010)

Scer\GAL4^da.G32, krz^S047819/krz[+], rl^Sem.S.UAS has partially lethal - majority die phenotype, suppressible by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Tipping et al., 2010)

Enhancer of

Statement

Reference

rl^Sem.S.UAS, Scer\GAL4^vg.int2.1 is an enhancer of visible phenotype of Mad^UAS.cNa, Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

NOT Suppressor of

Statement

Reference

rl^Sem.S.UAS, Scer\GAL4^GMR32D05 is a non-suppressor of decreased cell number | larval stage phenotype of Ama^HMS00297, Scer\GAL4^GMR32D05

(Moucaud et al., 2024)

Scer\GAL4^C587/rl^Sem.S.UAS is a non-suppressor of abnormal size | recessive | male | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has wing vein phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^GD8339

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has wing blade phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^KK101471

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has wing vein phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^KK101471

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has wing blade phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^GD8339

(Ashton-Beaucage et al., 2016)

Scer\GAL4^da.G32, rl^Sem.S.UAS has wing phenotype, enhanceable by krz^S047819/krz[+]

(Tipping et al., 2010)

Scer\GAL4^da.G32, rl^Sem.S.UAS has wing vein phenotype, enhanceable by krz^S047819/krz[+]

(Tipping et al., 2010)

Scer\GAL4^da.G32, rl^Sem.S.UAS has eye phenotype, enhanceable by krz^S047819/krz[+]

(Tipping et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, rl^Sem.S.UAS has wing blade phenotype, suppressible | partially by Scer\GAL4^salm.EPv/Usp47^GD10642

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, rl^Sem.S.UAS has wing vein | ectopic phenotype, suppressible | partially by Scer\GAL4^salm.EPv/Usp47^GD10642

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, rl^Sem.S.UAS has wing blade phenotype, suppressible | partially by Scer\GAL4^salm.EPv/Usp47^NIG.5486R

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, rl^Sem.S.UAS has wing vein | ectopic phenotype, suppressible | partially by Scer\GAL4^salm.EPv/Usp47^NIG.5486R

(Ashton-Beaucage et al., 2016)

Scer\GAL4^da.G32, krz^S047819/krz[+], rl^Sem.S.UAS has wing vein phenotype, suppressible by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Tipping et al., 2010)

Scer\GAL4^da.G32, krz^S047819/krz[+], rl^Sem.S.UAS has eye phenotype, suppressible by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Tipping et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^da.G32, krz^S047819/krz[+], rl^Sem.S.UAS has wing phenotype, non-suppressible by krz^{UAS.Tag:GS-TAP}/Scer\GAL4^da.G32

(Tipping et al., 2010)

Enhancer of

Statement

Reference

rl^Sem.S.UAS, Scer\GAL4^vg.int2.1 is an enhancer of wing phenotype of Mad^UAS.cNa, Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

rl^Sem.S.UAS, Scer\GAL4^vg.int2.1 is an enhancer of wing vein | ectopic phenotype of Mad^UAS.cNa, Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

NOT Suppressor of

Statement

Reference

rl^Sem.S.UAS, Scer\GAL4^GMR32D05 is a non-suppressor of myoblast | larval stage | decreased number phenotype of Ama^HMS00297, Scer\GAL4^GMR32D05

(Moucaud et al., 2024)

Scer\GAL4^C587/rl^Sem.S.UAS is a non-suppressor of gonad | male | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wein material in the adult wing characteristic for flies expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^salm.EPv is partially suppressed by co-expression of either Usp47^GD10642 or Usp47^NIG.5486R. The ameliorated phenotype of Scer\GAL4^salm.EPv,rl^{Sem.S.Scer\UAS};Usp47^GD10642 flies can be worsened again by co-expression of either poe^GD8339 or poe^KK101471 : the severity of their ectopic venation phenotype is comparable to that of flies expressing rl^{Sem.S.Scer\UAS} only.

(Ashton-Beaucage et al., 2016)

Expression of rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 in a krz^S047819/+ mutant background results in the development of even more additional veins in the wing and a further reduction in wing size.

Expression of rl^{Sem.S.Scer\UAS} and krz^{Scer\UAS.T:Zzzz\GS-TAP} under the control of Scer\GAL4^da.G32 in a krz^S047819/+ mutant background restores the wing vein phenotype to that seen in animals expressing just rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 in a wild-type background. Wing size is larger in these flies compared to animals expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 in a wild-type background.

The mild irregularities in ommatidial pattern observed in animals expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 is strongly enhanced in a krz^S047819/+ mutant background, and smaller rougher eyes are observed.

Expression of krz^{Scer\UAS.T:Zzzz\GS-TAP} restores the regular ommatidial pattern and size of the eye in animals expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 in a krz^S047819/+ mutant background.

The survival rate of animals expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 drops to 8% in a krz^S047819/+ mutant background.

The survival rate of animals expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^da.G32 in a krz^S047819/+ mutant background increases to 38% when krz^{Scer\UAS.T:Zzzz\GS-TAP} is co-expressed.

(Tipping et al., 2010)

Co-expression of rl^{Sem.S.Scer\UAS} enhances the mutant wing phenotype caused by expression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^vg.int2.1.

(Zeng et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59006

y¹ w^*; P{UAS-rl^Sem.S}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

ERK^CA

(Mattila et al., 2018)

MAPK^ACT

(Fernandes et al., 2017)

rl^{Sem.S.Scer\UAS}

rl^Sem.S.UAS

rl^Sem

(Azuma et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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