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General Information
Symbol
Dmel\parkdsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0151644
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of park sequences which are arranged in an inverted repeat. This construct should produce park dsRNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of parkdsRNA.UAS under the control of Scer\GAL4Ddc.PL results in decreased lifespan and severely impaired adult locomotion, Scer\GAL4GMR.PF-driven expression leads to reduced number of ommatidia in adult eyes and disrupted ommatidial lattice.

Whole-eye knockdown of park, through expression of parkdsRNA.Scer\UAS under the control of Scer\GAL4ey.PH leads to lipid droplet accumulation. Aconitase activity is reduced to less than 50% of wild-type in these mutants and there is a strong correlation between decreased aconitase enzymatic activity and the number of lipid droplets per ommatidium, suggesting reactive oxygen species may play a role in lipid droplet accumulation.

Expression of parkdsRNA.Scer\UAS under the control of Scer\GAL4Ddc.PL does not significantly affect the number of dopaminergic neurons in the dorsomedial clusters of the brain in 30 day old flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The reduced adult lifespan along with the impaired climbing ability characteristic for flies expressing parkdsRNA.UAS under the control of Scer\GAL4Ddc.PL is significantly enhanced by co-expression of BuffyUAS.cQa. In contrast, the disrupted ommatidial lattice and loss of ommatidia of flies expressing parkdsRNA.UAS driven by Scer\GAL4GMR.PF is ameliorated by BuffyUAS.cQa.

Xenogenetic Interactions
Statement
Reference

Coexpression of parkdsRNA.Scer\UAS results in a dosage-dependent acceleration of the dopaminergic (DA) neuron degeneration that is seen in flies expressing Hsap\GPR37Scer\UAS.cYa under the control of Scer\GAL4Ddc.PL; flies co-expressing Hsap\GPR37Scer\UAS.cYa and two copies of parkdsRNA.Scer\UAS show the degeneration phenotype at 14 days of age, whereas flies expressing Hsap\GPR37Scer\UAS.cYa alone or Hsap\GPR37Scer\UAS.cYa and one copy of parkdsRNA.Scer\UAS have a relatively normal number of DA neurons in the dorsomedial clusters. The severity of the DA neuron degeneration phenotype is also enhanced.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
parkdsRNA.Scer\UAS
parkdsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (4)