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General Information
Symbol
Dmel\lolaORC4
Species
D. melanogaster
Name
FlyBase ID
FBal0151749
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C10508949T

Reported nucleotide change:

C34341T

Amino acid change:

Q771term | lola-PAA; Q771term | lola-PI

Reported amino acid change:

Q771V|lola-PI

Comment:

Q771 is replaced with a stop codon in the C5-8/V21-22 isoform.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: E771term.

Nucleotide substitution: T34341C.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

lolaORC4/lola22.05 transheterozygous embryos display a significant increase in defects in the fusion together and compaction of primordial germ cells and somatic gonadal precursor cells, as compared to controls.

Females containing homozygous germline clones show defects in stage 14 egg chambers, including dumpless egg chambers (egg chambers in which the nurse cells have failed to transfer their cytoplasmic contents to the oocyte, have intact nuclei and do not appear to be undergoing programmed cell death) and egg chambers with persisting nurse cell nuclei (egg chambers in which nurse cells have transferred their cytoplasmic contents to the oocyte, but their nuclei have failed to undergo programmed cell death including nuclear condensation). Most of the dumpless egg chambers do not contain any TUNEL-positive nurse cells.

Mid-stage degenerating egg chambers from nutrient deprived females containing homozygous germline clones are not TUNEL-positive (in contrast to egg chambers in nutrient deprived control females).

18% of mid-stage egg chambers containing homozygous germline clones show a protrusion of the nurse cell lamina through the ring canal.

lolaORC4 MARCM clones do not show obvious projection neuron dendritic targeting defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhancer of
Statement
Reference

lola[+]/lolaORC4 is a non-enhancer of wing margin phenotype of ct53d

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)