FlyBase Allele Report: Dmel\Smurf[UAS.Tag:FLAG]

General Information

Symbol

Dmel\Smurf^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0151755

Feature type

allele

Associated gene

Dmel\Smurf

Associated Insertion(s)

Carried in Construct

P{UAS-Smurf.FLAG}

Also Known As

UAS-dSmurf

Key Links

Transgenic product class

wild_type

(Liang et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Liang et al., 2003)

Mutagen

in vitro construct

(Liang et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Smurf.FLAG}

Cytology

Description

UASt regulatory sequences drive expression of Smurf tagged with Tag:FLAG.

(Liang et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Smurf

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape, with Scer\GAL4^{VP16.nanos.UTR}

(Deshpande et al., 2014)

lethal - all die before end of P-stage, with Scer\GAL4^ptc-559.1

(Liang et al., 2003)

some die during pharate adult stage, with Scer\GAL4^ptc-559.1

(Liang et al., 2003)

Phenotype Manifest In

germline cell, with Scer\GAL4^{VP16.nanos.UTR}

(Deshpande et al., 2014)

leg, with Scer\GAL4^ptc-559.1

(Liang et al., 2003)

spectrosome, with Scer\GAL4^{VP16.nanos.UTR}

(Deshpande et al., 2014)

wing, with Scer\GAL4^ptc-559.1

(Liang et al., 2003)

Detailed Description

Statement

Reference

Expression of Smurf^{Scer\UAS.T:Zzzz\FLAG} ectopically in the germ cells, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, results in defects in primordial germ cells, with only 37% displaying wild-type spectrosomes, with the remaining germ cells showing fragmented, faint or missing spectrosomes.

(Deshpande et al., 2014)

Expression of lack^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ptc-559.1 results in pharate adults with rudimentary wings and truncated legs.

(Liang et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Smurf^{Scer\UAS.T:Zzzz\FLAG}

Smurf^UAS.Tag:FLAG

lack^{Scer\UAS.T:Zzzz\FLAG}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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