FlyBase Allele Report: Dmel\hpo[UAS.Tag:FLAG]

General Information

Symbol

Dmel\hpo^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0151842

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{UAS-dMST.FLAG}

Also Known As

UAS-hpo, UAS-FLAG-hpo

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Jia et al., 2003)

in vitro construct - coding region fusion

(Jia et al., 2003)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-dMST.FLAG}

Cytology

Nature of the lesion

Statement

Reference

UASt sequences drive expression of hpo tagged with Tag:FLAG.

(Jia et al., 2003)

Allele components

Product class / Tool use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Tagged with

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing disc | somatic clone, with Scer\GAL4^Act5C.PI

(Oh and Irvine, 2008)

Detailed Description

Statement

Reference

Clones expressing hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PI are not recovered in the wing disc.

(Oh and Irvine, 2008)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

hpo^UAS.Tag:FLAG, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF is a suppressor of lethal phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh and Irvine, 2009)

hpo^UAS.Tag:FLAG, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF, wts^UAS.Tag:MYC is a suppressor of lethal phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh and Irvine, 2009)

hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, Scer\GAL4^GMR.PU is a suppressor | partially of neoplasia | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, Scer\GAL4^GMR.PU is a suppressor | partially of neoplasia | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, Scer\GAL4^Act5C.PI, wts^UAS.Tag:MYC is a suppressor of neoplasia | somatic clone | larval stage phenotype of Scer\GAL4^Act5C.PI, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, wts^UAS.Tag:MYC, Scer\GAL4^GMR.PU is a suppressor of neoplasia | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

NOT Suppressor of

Statement

Reference

hpo^UAS.Tag:FLAG/Scer\GAL4^GMR.PU is a non-suppressor of neoplasia | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

Other

Statement

Reference

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S250A.UAS.Tag:V5} has lethal phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S168A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S168A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{UAS.cOa.Tag:V5} has lethal - all die during P-stage phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.S168A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S168A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S168A.S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S168A.S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.S168A.S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{UAS.cOa.Tag:V5} has lethal - all die during P-stage phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S168A.S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.UAS.Tag:V5} has lethal phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S169A.S172A.UAS.Tag:V5} has lethal phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, hpo^UAS.Tag:FLAG has wing disc | somatic clone phenotype, suppressible by Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI/yki^{S168A.UAS.EGFP,Tag:HA}

(Oh and Irvine, 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, hpo^UAS.Tag:FLAG has wing disc | somatic clone phenotype, non-suppressible by Scer\GAL4^Act5C.PI/yki^{UAS.EGFP,Tag:HA}/Scer\GAL4^Act5C.PI

(Oh and Irvine, 2008)

Suppressor of

Statement

Reference

hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, Scer\GAL4^GMR.PU is a suppressor | partially of eye disc | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, Scer\GAL4^GMR.PU is a suppressor | partially of eye disc | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, Scer\GAL4^Act5C.PI, wts^UAS.Tag:MYC is a suppressor of wing pouch | somatic clone | larval stage phenotype of Scer\GAL4^Act5C.PI, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, wts^UAS.Tag:MYC, Scer\GAL4^GMR.PU is a suppressor of eye disc | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

NOT Suppressor of

Statement

Reference

hpo^UAS.Tag:FLAG/Scer\GAL4^GMR.PU is a non-suppressor of eye disc | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

Other

Statement

Reference

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S250A.UAS.Tag:V5} has wing disc | somatic clone phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S168A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S168A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.S168A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S168A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S168A.S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S168A.S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC has wing disc | somatic clone phenotype

(Oh and Irvine, 2008)

Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S168A.UAS.EGFP,Tag:HA} has wing disc | somatic clone phenotype

(Oh and Irvine, 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of yki^{S111A.Scer\UAS.T:SV5\V5} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with very small eyes.

Co-expression of yki^{S250A.Scer\UAS.T:SV5\V5} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with small eyes.

Co-expression of yki^{S111A.S250A.Scer\UAS.T:SV5\V5} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with small eyes.

Co-expression of yki^{S111A.S250A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with very small eyes.

Co-expression of yki^{S168A.Scer\UAS.T:SV5\V5} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with greatly enlarged eyes.

Co-expression of yki^{S168A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with enlarged eyes.

Co-expression of yki^{S111A.S168A.Scer\UAS.T:SV5\V5} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with greatly enlarged eyes (but less so than in the absence of hpo^{Scer\UAS.T:Zzzz\FLAG}).

Co-expression of yki^{S111A.S168A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with greatly enlarged eyes (but less so than in the absence of wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG}).

Co-expression of yki^{S168A.S250A.Scer\UAS.T:SV5\V5} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with greatly enlarged eyes (but less so than in the absence of hpo^{Scer\UAS.T:Zzzz\FLAG}).

Co-expression of yki^{S168A.S250A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with greatly enlarged eyes (but less so than in the absence of wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG}).

Co-expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with enlarged and highly folded eyes.

Co-expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with enlarged and highly folded eyes.

Clones in the wing disc co-expressing yki^{S111A.S250A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PI do not survive well.

Large round clones are still observed in the wing disc when yki^{S168A.Scer\UAS.T:SV5\V5} is co-expressed with wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PI in the clones.

Co-expression of ex^Scer\UAS.cBa in clones expressing yki^{S168A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PI decreases the size and number of clones recovered in the wing disc.

Clones co-expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} ex^Scer\UAS.cBa, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PI in the wing disc are large and round.

(Oh and Irvine, 2009)

Clones of cells in the wing or eye disc in which wts^{Scer\UAS.T:Hsap\MYC}, hpo^{Scer\UAS.T:Zzzz\FLAG} and ex^Scer\UAS.cBa are over-expressed together with yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} generally overgrow compared to control clones, but can be less overgrown than yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5}-expressing clones alone. This is most evident in the wing pouch.

The overgrowth and folding of the eye disc induced by Scer\GAL4^GMR.PU-mediated expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is largely suppressed by co-expression of wts^{Scer\UAS.T:Hsap\MYC}, hpo^{Scer\UAS.T:Zzzz\FLAG} and ex^Scer\UAS.cBa.

The overgrowth and folding of the eye disc induced by Scer\GAL4^GMR.PU-mediated expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is not suppressed by co-expression of hpo^{Scer\UAS.T:Zzzz\FLAG} alone.

The overgrowth and folding of the eye disc induced by Scer\GAL4^GMR.PU-mediated expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is partly suppressed by co-expression of wts^{Scer\UAS.T:Hsap\MYC}, hpo^{Scer\UAS.T:Zzzz\FLAG} together, or hpo^{Scer\UAS.T:Zzzz\FLAG} and ex^Scer\UAS.cBa together.

(Oh et al., 2009)

Clones co-expressing hpo^{Scer\UAS.T:Zzzz\FLAG} and yki^{Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PI in the wing disc are tiny and rarely detected.

Clones co-expressing hpo^{Scer\UAS.T:Zzzz\FLAG} and yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PI are recovered in the wing disc.

Clones co-expressing hpo^{Scer\UAS.T:Zzzz\FLAG} and wts^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PI are not recovered in the wing disc.

Clones co-expressing hpo^{Scer\UAS.T:Zzzz\FLAG}, yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} and wts^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PI are not recovered in the wing disc.

(Oh and Irvine, 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

44254

y¹ w^*; wg^Sp-1/CyO; P{UAS-dMST.FLAG}3/TM2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hpo^{Scer\UAS.T:Zzzz\FLAG}

hpo^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (9)

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